The Pepper Mitogen-Activated Protein Kinase CaMAPK7 Acts as a Positive Regulator in Response to Ralstonia solanacearum Infection
Mitogen-activated protein kinase (MAPK) pathways play a vital role in multiple plant processes, including growth, development, and stress signaling, but their involvement in response to Ralstonia solanacearum is poorly understood, particularly in pepper plants. Herein, CaMAPK7 was identified from th...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-07-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.664926/full |
| _version_ | 1818669301799845888 |
|---|---|
| author | Lanping Shi Lanping Shi Kan Zhang Kan Zhang Linjing Xie Linjing Xie Mingxing Yang Mingxing Yang Baixue Xie Baixue Xie Shuilin He Shuilin He Zhiqin Liu Zhiqin Liu |
| author_facet | Lanping Shi Lanping Shi Kan Zhang Kan Zhang Linjing Xie Linjing Xie Mingxing Yang Mingxing Yang Baixue Xie Baixue Xie Shuilin He Shuilin He Zhiqin Liu Zhiqin Liu |
| author_sort | Lanping Shi |
| collection | DOAJ |
| description | Mitogen-activated protein kinase (MAPK) pathways play a vital role in multiple plant processes, including growth, development, and stress signaling, but their involvement in response to Ralstonia solanacearum is poorly understood, particularly in pepper plants. Herein, CaMAPK7 was identified from the pepper genome and functionally analyzed. The accumulations of CaMAPK7 transcripts and promoter activities were both significantly induced in response to R. solanacearum strain FJC100301 infection, and exogenously applied phytohormones, including methyl jasmonate (MeJA), brassinolide (BR), salicylic acid (SA), and ethephon (ETN), were decreased by abscisic acid (ABA) treatment. Virus-induced gene silencing (VIGS) of CaMAPK7 significantly enhanced the susceptibility of pepper plants to infection by R. solanacearum and downregulated the defense-related marker genes, including CaDEF1, CaPO2, CaSAR82A, and CaWRKY40. In contrast, the ectopic overexpression of CaMAPK7 in transgenic tobacco enhanced resistance to R. solanacearum and upregulated the defense-associated marker genes, including NtHSR201, NtHSR203, NtPR4, PR1a/c, NtPR1b, NtCAT1, and NtACC. Furthermore, transient overexpression of CaMAPK7 in pepper leaves triggered intensive hypersensitive response (HR)-like cell death, H2O2 accumulation, and enriched CaWRKY40 at the promoters of its target genes and drove their transcript accumulations, including CaDEF1, CaPO2, and CaSAR82A. Taken together, these data indicate that R. solanacearum infection induced the expression of CaMAPK7, which indirectly modifies the binding of CaWRKY40 to its downstream targets, including CaDEF1, CaPO2, and CaSAR82A, ultimately leading to the activation of pepper immunity against R. solanacearum. The protein that responds to CaMAPK7 in pepper plants should be isolated in the future to build a signaling bridge between CaMAPK7 and CaWRKY40. |
| first_indexed | 2024-12-17T06:50:03Z |
| format | Article |
| id | doaj.art-aed0298b0f5c4894a42ade292cfa0bda |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-17T06:50:03Z |
| publishDate | 2021-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-aed0298b0f5c4894a42ade292cfa0bda2022-12-21T21:59:38ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-07-011210.3389/fmicb.2021.664926664926The Pepper Mitogen-Activated Protein Kinase CaMAPK7 Acts as a Positive Regulator in Response to Ralstonia solanacearum InfectionLanping Shi0Lanping Shi1Kan Zhang2Kan Zhang3Linjing Xie4Linjing Xie5Mingxing Yang6Mingxing Yang7Baixue Xie8Baixue Xie9Shuilin He10Shuilin He11Zhiqin Liu12Zhiqin Liu13Fujian Provincial Key Laboratory of Applied Genetics, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, ChinaFujian Provincial Key Laboratory of Applied Genetics, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, ChinaFujian Provincial Key Laboratory of Applied Genetics, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, ChinaFujian Provincial Key Laboratory of Applied Genetics, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, ChinaFujian Provincial Key Laboratory of Applied Genetics, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, ChinaFujian Provincial Key Laboratory of Applied Genetics, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, ChinaFujian Provincial Key Laboratory of Applied Genetics, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, ChinaMitogen-activated protein kinase (MAPK) pathways play a vital role in multiple plant processes, including growth, development, and stress signaling, but their involvement in response to Ralstonia solanacearum is poorly understood, particularly in pepper plants. Herein, CaMAPK7 was identified from the pepper genome and functionally analyzed. The accumulations of CaMAPK7 transcripts and promoter activities were both significantly induced in response to R. solanacearum strain FJC100301 infection, and exogenously applied phytohormones, including methyl jasmonate (MeJA), brassinolide (BR), salicylic acid (SA), and ethephon (ETN), were decreased by abscisic acid (ABA) treatment. Virus-induced gene silencing (VIGS) of CaMAPK7 significantly enhanced the susceptibility of pepper plants to infection by R. solanacearum and downregulated the defense-related marker genes, including CaDEF1, CaPO2, CaSAR82A, and CaWRKY40. In contrast, the ectopic overexpression of CaMAPK7 in transgenic tobacco enhanced resistance to R. solanacearum and upregulated the defense-associated marker genes, including NtHSR201, NtHSR203, NtPR4, PR1a/c, NtPR1b, NtCAT1, and NtACC. Furthermore, transient overexpression of CaMAPK7 in pepper leaves triggered intensive hypersensitive response (HR)-like cell death, H2O2 accumulation, and enriched CaWRKY40 at the promoters of its target genes and drove their transcript accumulations, including CaDEF1, CaPO2, and CaSAR82A. Taken together, these data indicate that R. solanacearum infection induced the expression of CaMAPK7, which indirectly modifies the binding of CaWRKY40 to its downstream targets, including CaDEF1, CaPO2, and CaSAR82A, ultimately leading to the activation of pepper immunity against R. solanacearum. The protein that responds to CaMAPK7 in pepper plants should be isolated in the future to build a signaling bridge between CaMAPK7 and CaWRKY40.https://www.frontiersin.org/articles/10.3389/fmicb.2021.664926/fullCapsicum annuummitogen-activated protein kinaseRalstonia solanacearumWRKY transcription factorpathogenicity |
| spellingShingle | Lanping Shi Lanping Shi Kan Zhang Kan Zhang Linjing Xie Linjing Xie Mingxing Yang Mingxing Yang Baixue Xie Baixue Xie Shuilin He Shuilin He Zhiqin Liu Zhiqin Liu The Pepper Mitogen-Activated Protein Kinase CaMAPK7 Acts as a Positive Regulator in Response to Ralstonia solanacearum Infection Frontiers in Microbiology Capsicum annuum mitogen-activated protein kinase Ralstonia solanacearum WRKY transcription factor pathogenicity |
| title | The Pepper Mitogen-Activated Protein Kinase CaMAPK7 Acts as a Positive Regulator in Response to Ralstonia solanacearum Infection |
| title_full | The Pepper Mitogen-Activated Protein Kinase CaMAPK7 Acts as a Positive Regulator in Response to Ralstonia solanacearum Infection |
| title_fullStr | The Pepper Mitogen-Activated Protein Kinase CaMAPK7 Acts as a Positive Regulator in Response to Ralstonia solanacearum Infection |
| title_full_unstemmed | The Pepper Mitogen-Activated Protein Kinase CaMAPK7 Acts as a Positive Regulator in Response to Ralstonia solanacearum Infection |
| title_short | The Pepper Mitogen-Activated Protein Kinase CaMAPK7 Acts as a Positive Regulator in Response to Ralstonia solanacearum Infection |
| title_sort | pepper mitogen activated protein kinase camapk7 acts as a positive regulator in response to ralstonia solanacearum infection |
| topic | Capsicum annuum mitogen-activated protein kinase Ralstonia solanacearum WRKY transcription factor pathogenicity |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.664926/full |
| work_keys_str_mv | AT lanpingshi thepeppermitogenactivatedproteinkinasecamapk7actsasapositiveregulatorinresponsetoralstoniasolanacearuminfection AT lanpingshi thepeppermitogenactivatedproteinkinasecamapk7actsasapositiveregulatorinresponsetoralstoniasolanacearuminfection AT kanzhang thepeppermitogenactivatedproteinkinasecamapk7actsasapositiveregulatorinresponsetoralstoniasolanacearuminfection AT kanzhang thepeppermitogenactivatedproteinkinasecamapk7actsasapositiveregulatorinresponsetoralstoniasolanacearuminfection AT linjingxie thepeppermitogenactivatedproteinkinasecamapk7actsasapositiveregulatorinresponsetoralstoniasolanacearuminfection AT linjingxie thepeppermitogenactivatedproteinkinasecamapk7actsasapositiveregulatorinresponsetoralstoniasolanacearuminfection AT mingxingyang thepeppermitogenactivatedproteinkinasecamapk7actsasapositiveregulatorinresponsetoralstoniasolanacearuminfection AT mingxingyang thepeppermitogenactivatedproteinkinasecamapk7actsasapositiveregulatorinresponsetoralstoniasolanacearuminfection AT baixuexie thepeppermitogenactivatedproteinkinasecamapk7actsasapositiveregulatorinresponsetoralstoniasolanacearuminfection AT baixuexie thepeppermitogenactivatedproteinkinasecamapk7actsasapositiveregulatorinresponsetoralstoniasolanacearuminfection AT shuilinhe thepeppermitogenactivatedproteinkinasecamapk7actsasapositiveregulatorinresponsetoralstoniasolanacearuminfection AT shuilinhe thepeppermitogenactivatedproteinkinasecamapk7actsasapositiveregulatorinresponsetoralstoniasolanacearuminfection AT zhiqinliu thepeppermitogenactivatedproteinkinasecamapk7actsasapositiveregulatorinresponsetoralstoniasolanacearuminfection AT zhiqinliu thepeppermitogenactivatedproteinkinasecamapk7actsasapositiveregulatorinresponsetoralstoniasolanacearuminfection AT lanpingshi peppermitogenactivatedproteinkinasecamapk7actsasapositiveregulatorinresponsetoralstoniasolanacearuminfection AT lanpingshi peppermitogenactivatedproteinkinasecamapk7actsasapositiveregulatorinresponsetoralstoniasolanacearuminfection AT kanzhang peppermitogenactivatedproteinkinasecamapk7actsasapositiveregulatorinresponsetoralstoniasolanacearuminfection AT kanzhang peppermitogenactivatedproteinkinasecamapk7actsasapositiveregulatorinresponsetoralstoniasolanacearuminfection AT linjingxie peppermitogenactivatedproteinkinasecamapk7actsasapositiveregulatorinresponsetoralstoniasolanacearuminfection AT linjingxie peppermitogenactivatedproteinkinasecamapk7actsasapositiveregulatorinresponsetoralstoniasolanacearuminfection AT mingxingyang peppermitogenactivatedproteinkinasecamapk7actsasapositiveregulatorinresponsetoralstoniasolanacearuminfection AT mingxingyang peppermitogenactivatedproteinkinasecamapk7actsasapositiveregulatorinresponsetoralstoniasolanacearuminfection AT baixuexie peppermitogenactivatedproteinkinasecamapk7actsasapositiveregulatorinresponsetoralstoniasolanacearuminfection AT baixuexie peppermitogenactivatedproteinkinasecamapk7actsasapositiveregulatorinresponsetoralstoniasolanacearuminfection AT shuilinhe peppermitogenactivatedproteinkinasecamapk7actsasapositiveregulatorinresponsetoralstoniasolanacearuminfection AT shuilinhe peppermitogenactivatedproteinkinasecamapk7actsasapositiveregulatorinresponsetoralstoniasolanacearuminfection AT zhiqinliu peppermitogenactivatedproteinkinasecamapk7actsasapositiveregulatorinresponsetoralstoniasolanacearuminfection AT zhiqinliu peppermitogenactivatedproteinkinasecamapk7actsasapositiveregulatorinresponsetoralstoniasolanacearuminfection |