Dietary <i>Weizmannia coagulans</i> Strain SANK70258 Ameliorates Coccidial Symptoms and Improves Intestinal Barrier Functions of Broilers by Modulating the Intestinal Immunity and the Gut Microbiota
To determine the mechanisms by which <i>Weizmannia coagulans</i> SANK70258 (WC) supplementation improved growth performance and coccidial symptoms, we assessed the gene expressions and the microbiota compositions in the small intestinal tissues and digestas of coccidium-infected broilers...
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MDPI AG
2023-01-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/12/1/96 |
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| author | Masanori Aida Ryouichi Yamada Toshiki Matsuo Itaru Taniguchi Shin-ichi Nakamura Takamitsu Tsukahara |
| author_facet | Masanori Aida Ryouichi Yamada Toshiki Matsuo Itaru Taniguchi Shin-ichi Nakamura Takamitsu Tsukahara |
| author_sort | Masanori Aida |
| collection | DOAJ |
| description | To determine the mechanisms by which <i>Weizmannia coagulans</i> SANK70258 (WC) supplementation improved growth performance and coccidial symptoms, we assessed the gene expressions and the microbiota compositions in the small intestinal tissues and digestas of coccidium-infected broilers previously given WC or lasalocid-A sodium (AM). WC supplementation significantly upregulated the gene expressions related to intestinal immunity and barrier functions, such as <i>IL17A</i>, <i>IL17F</i>, <i>IL10</i>, <i>cathelicidin-2</i> and <i>pIgR</i>. Body weights, and <i>Claudin-1</i> and <i>IL10</i> expressions were positively correlated (r = 0.41, <i>p</i> < 0.05 and r = 0.37, <i>p</i> = 0.06, respectively), whereas lesion scores of the small intestine and <i>IL17A</i> expression were negatively correlated (r = −0.33, <i>p</i> = 0.09). The microbiota analysis detected that genus <i>Alistipes</i> was more abundant in WC-supplemented broilers than in control, and positively correlated with body weights and <i>Claudin-1</i> expression (r = 0.61, <i>p</i> < 0.05 and r = 0.51, <i>p</i> < 0.05, respectively). Intriguingly, genus <i>Enterococcus</i> was most abundant in WC-supplemented broilers and positively correlated with <i>IL17A</i> expression (r = 0.49, <i>p</i> < 0.05). Interestingly, <i>Escherichia-Shigella</i> was significantly more abundant in the small intestinal digestas of AM-administered broilers than in those of control. To summarize, WC supplementation modulated and immunostimulated the microbiotas of broilers, specifically genera <i>Alistipes</i> and <i>Enterococcus</i>, which led to the improvement of weight gain and coccidial symptoms, without disrupting the intestinal microbiota compositions, as AM did. |
| first_indexed | 2024-03-09T11:29:47Z |
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| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T11:29:47Z |
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| spelling | doaj.art-aed2f274333e473184521bac076ec7bb2023-11-30T23:53:40ZengMDPI AGPathogens2076-08172023-01-011219610.3390/pathogens12010096Dietary <i>Weizmannia coagulans</i> Strain SANK70258 Ameliorates Coccidial Symptoms and Improves Intestinal Barrier Functions of Broilers by Modulating the Intestinal Immunity and the Gut MicrobiotaMasanori Aida0Ryouichi Yamada1Toshiki Matsuo2Itaru Taniguchi3Shin-ichi Nakamura4Takamitsu Tsukahara5Science & Innovation Center, Mitsubishi Chemical Corporation, Yokohama 227-8502, Kanagawa, JapanScience & Innovation Center, Mitsubishi Chemical Corporation, Yokohama 227-8502, Kanagawa, JapanMitsubishi Chemical Corporation, Tokyo 100-8251, JapanMitsubishi Chemical Corporation, Tokyo 100-8251, JapanKyoto Institute of Nutrition & Pathology, Ujitawara 610-0231, Kyoto, JapanKyoto Institute of Nutrition & Pathology, Ujitawara 610-0231, Kyoto, JapanTo determine the mechanisms by which <i>Weizmannia coagulans</i> SANK70258 (WC) supplementation improved growth performance and coccidial symptoms, we assessed the gene expressions and the microbiota compositions in the small intestinal tissues and digestas of coccidium-infected broilers previously given WC or lasalocid-A sodium (AM). WC supplementation significantly upregulated the gene expressions related to intestinal immunity and barrier functions, such as <i>IL17A</i>, <i>IL17F</i>, <i>IL10</i>, <i>cathelicidin-2</i> and <i>pIgR</i>. Body weights, and <i>Claudin-1</i> and <i>IL10</i> expressions were positively correlated (r = 0.41, <i>p</i> < 0.05 and r = 0.37, <i>p</i> = 0.06, respectively), whereas lesion scores of the small intestine and <i>IL17A</i> expression were negatively correlated (r = −0.33, <i>p</i> = 0.09). The microbiota analysis detected that genus <i>Alistipes</i> was more abundant in WC-supplemented broilers than in control, and positively correlated with body weights and <i>Claudin-1</i> expression (r = 0.61, <i>p</i> < 0.05 and r = 0.51, <i>p</i> < 0.05, respectively). Intriguingly, genus <i>Enterococcus</i> was most abundant in WC-supplemented broilers and positively correlated with <i>IL17A</i> expression (r = 0.49, <i>p</i> < 0.05). Interestingly, <i>Escherichia-Shigella</i> was significantly more abundant in the small intestinal digestas of AM-administered broilers than in those of control. To summarize, WC supplementation modulated and immunostimulated the microbiotas of broilers, specifically genera <i>Alistipes</i> and <i>Enterococcus</i>, which led to the improvement of weight gain and coccidial symptoms, without disrupting the intestinal microbiota compositions, as AM did.https://www.mdpi.com/2076-0817/12/1/96<i>Weizmannia coagulans</i><i>Eimeria</i>broilersanti-inflammatory cytokinesintestinal barrier function |
| spellingShingle | Masanori Aida Ryouichi Yamada Toshiki Matsuo Itaru Taniguchi Shin-ichi Nakamura Takamitsu Tsukahara Dietary <i>Weizmannia coagulans</i> Strain SANK70258 Ameliorates Coccidial Symptoms and Improves Intestinal Barrier Functions of Broilers by Modulating the Intestinal Immunity and the Gut Microbiota Pathogens <i>Weizmannia coagulans</i> <i>Eimeria</i> broilers anti-inflammatory cytokines intestinal barrier function |
| title | Dietary <i>Weizmannia coagulans</i> Strain SANK70258 Ameliorates Coccidial Symptoms and Improves Intestinal Barrier Functions of Broilers by Modulating the Intestinal Immunity and the Gut Microbiota |
| title_full | Dietary <i>Weizmannia coagulans</i> Strain SANK70258 Ameliorates Coccidial Symptoms and Improves Intestinal Barrier Functions of Broilers by Modulating the Intestinal Immunity and the Gut Microbiota |
| title_fullStr | Dietary <i>Weizmannia coagulans</i> Strain SANK70258 Ameliorates Coccidial Symptoms and Improves Intestinal Barrier Functions of Broilers by Modulating the Intestinal Immunity and the Gut Microbiota |
| title_full_unstemmed | Dietary <i>Weizmannia coagulans</i> Strain SANK70258 Ameliorates Coccidial Symptoms and Improves Intestinal Barrier Functions of Broilers by Modulating the Intestinal Immunity and the Gut Microbiota |
| title_short | Dietary <i>Weizmannia coagulans</i> Strain SANK70258 Ameliorates Coccidial Symptoms and Improves Intestinal Barrier Functions of Broilers by Modulating the Intestinal Immunity and the Gut Microbiota |
| title_sort | dietary i weizmannia coagulans i strain sank70258 ameliorates coccidial symptoms and improves intestinal barrier functions of broilers by modulating the intestinal immunity and the gut microbiota |
| topic | <i>Weizmannia coagulans</i> <i>Eimeria</i> broilers anti-inflammatory cytokines intestinal barrier function |
| url | https://www.mdpi.com/2076-0817/12/1/96 |
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