Enzyme-Free Signal Amplification Strategy via Chaperone Copolymer-Accelerated Hybridization for Highly Sensitive Detection of Adenosine
Adenosine is a vital biological small molecule that regulates various physiological processes in the human body. A high expression of adenosine in cells can facilitate tumor growth. Therefore, detecting adenosine is crucial for early disease diagnosis. In this paper, we designed a fluorescent biosen...
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MDPI AG
2023-10-01
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| Series: | Chemosensors |
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| Online Access: | https://www.mdpi.com/2227-9040/11/10/522 |
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| author | Yazhen Liao Yuxing Yang Yang Qing Jie Du |
| author_facet | Yazhen Liao Yuxing Yang Yang Qing Jie Du |
| author_sort | Yazhen Liao |
| collection | DOAJ |
| description | Adenosine is a vital biological small molecule that regulates various physiological processes in the human body. A high expression of adenosine in cells can facilitate tumor growth. Therefore, detecting adenosine is crucial for early disease diagnosis. In this paper, we designed a fluorescent biosensor for the sensitive detection of adenosine based on the cationic comb-type copolymer PLL-<i>g</i>-Dex for assisted rapid hybridization of nucleic acids at room temperature. In this strategy, adenosine preferentially binds to the aptamer immobilized on the surface of magnetic nanobeads, releasing free aDNA in solution as the primer strand, which rapidly forms DNA nanowires with auxiliary probes of bDNA with the assistance of PLL-<i>g</i>-Dex. SYBR Green I is embedded in DNA duplexes to generate strong fluorescence. The experimental results showed that PLL-<i>g</i>-Dex promotes DNA hybridization reactions at room temperature to form ultra-long DNA nanowires, thus achieving signal amplification and shortening the detection time. In addition, magnetic nanobeads can reduce the background signal during the reaction. Compared with several previous studies on the fluorescence detection of adenosine, this strategy has a lower detection limit of 2.32 nM. Furthermore, this novel system exhibited a good detection performance even under complex environments, such as serum, providing some reference for the quantitative detection of adenosine in early disease diagnosis. |
| first_indexed | 2024-03-10T21:21:50Z |
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| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-03-10T21:21:50Z |
| publishDate | 2023-10-01 |
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| series | Chemosensors |
| spelling | doaj.art-aed653be83954dee9ace2b9924b850fa2023-11-19T16:03:45ZengMDPI AGChemosensors2227-90402023-10-01111052210.3390/chemosensors11100522Enzyme-Free Signal Amplification Strategy via Chaperone Copolymer-Accelerated Hybridization for Highly Sensitive Detection of AdenosineYazhen Liao0Yuxing Yang1Yang Qing2Jie Du3State Key Laboratory of Marine Resource Utilization in South China Sea, College of Materials Science and Engineering, Hainan University, Haikou 570228, ChinaState Key Laboratory of Marine Resource Utilization in South China Sea, College of Materials Science and Engineering, Hainan University, Haikou 570228, ChinaState Key Laboratory of Marine Resource Utilization in South China Sea, College of Materials Science and Engineering, Hainan University, Haikou 570228, ChinaState Key Laboratory of Marine Resource Utilization in South China Sea, College of Materials Science and Engineering, Hainan University, Haikou 570228, ChinaAdenosine is a vital biological small molecule that regulates various physiological processes in the human body. A high expression of adenosine in cells can facilitate tumor growth. Therefore, detecting adenosine is crucial for early disease diagnosis. In this paper, we designed a fluorescent biosensor for the sensitive detection of adenosine based on the cationic comb-type copolymer PLL-<i>g</i>-Dex for assisted rapid hybridization of nucleic acids at room temperature. In this strategy, adenosine preferentially binds to the aptamer immobilized on the surface of magnetic nanobeads, releasing free aDNA in solution as the primer strand, which rapidly forms DNA nanowires with auxiliary probes of bDNA with the assistance of PLL-<i>g</i>-Dex. SYBR Green I is embedded in DNA duplexes to generate strong fluorescence. The experimental results showed that PLL-<i>g</i>-Dex promotes DNA hybridization reactions at room temperature to form ultra-long DNA nanowires, thus achieving signal amplification and shortening the detection time. In addition, magnetic nanobeads can reduce the background signal during the reaction. Compared with several previous studies on the fluorescence detection of adenosine, this strategy has a lower detection limit of 2.32 nM. Furthermore, this novel system exhibited a good detection performance even under complex environments, such as serum, providing some reference for the quantitative detection of adenosine in early disease diagnosis.https://www.mdpi.com/2227-9040/11/10/522adenosinechaperone copolymerfluorescence detectionsignal amplification |
| spellingShingle | Yazhen Liao Yuxing Yang Yang Qing Jie Du Enzyme-Free Signal Amplification Strategy via Chaperone Copolymer-Accelerated Hybridization for Highly Sensitive Detection of Adenosine Chemosensors adenosine chaperone copolymer fluorescence detection signal amplification |
| title | Enzyme-Free Signal Amplification Strategy via Chaperone Copolymer-Accelerated Hybridization for Highly Sensitive Detection of Adenosine |
| title_full | Enzyme-Free Signal Amplification Strategy via Chaperone Copolymer-Accelerated Hybridization for Highly Sensitive Detection of Adenosine |
| title_fullStr | Enzyme-Free Signal Amplification Strategy via Chaperone Copolymer-Accelerated Hybridization for Highly Sensitive Detection of Adenosine |
| title_full_unstemmed | Enzyme-Free Signal Amplification Strategy via Chaperone Copolymer-Accelerated Hybridization for Highly Sensitive Detection of Adenosine |
| title_short | Enzyme-Free Signal Amplification Strategy via Chaperone Copolymer-Accelerated Hybridization for Highly Sensitive Detection of Adenosine |
| title_sort | enzyme free signal amplification strategy via chaperone copolymer accelerated hybridization for highly sensitive detection of adenosine |
| topic | adenosine chaperone copolymer fluorescence detection signal amplification |
| url | https://www.mdpi.com/2227-9040/11/10/522 |
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