lncRNA TUG1 regulates hyperuricemia-induced renal <?A3B2 pi6?>fibrosis in a rat model
Renal fibrosis is most common among chronic kidney diseases. Molecular studies have shown that long noncoding RNAs (lncRNAs) and microRNAs (miRNAs) participate in renal fibrosis, while the roles of lncRNA taurine upregulated gene 1 (TUG1) and miR-140-3p in hyperuricemia-induced renal fibrosis remain...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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China Science Publishing & Media Ltd.
2022-09-01
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| Series: | Acta Biochimica et Biophysica Sinica |
| Subjects: | |
| Online Access: | https://www.sciengine.com/doi/10.3724/abbs.2022128 |
| _version_ | 1797635365076467712 |
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| author | Zhang Ying Zhang Haizhen Hu Langtao Wei Jiali Ma Chunyang |
| author_facet | Zhang Ying Zhang Haizhen Hu Langtao Wei Jiali Ma Chunyang |
| author_sort | Zhang Ying |
| collection | DOAJ |
| description | Renal fibrosis is most common among chronic kidney diseases. Molecular studies have shown that long noncoding RNAs (lncRNAs) and microRNAs (miRNAs) participate in renal fibrosis, while the roles of lncRNA taurine upregulated gene 1 (TUG1) and miR-140-3p in hyperuricemia-induced renal fibrosis remain less investigated. In this study, a rat hyperuricemia model is constructed by oral administration of adenine. TUG1, miR-140-3p, and cathepsin D (CtsD) expression levels in rat models are measured. After altering TUG1, miR-140-3p, or CtsD expression in modelled rats, biochemical indices, including uric acid (UA), serum creatine (SCr), blood urea nitrogen (BUN), and 24-h urine protein are detected, pathological changes in the renal tissues, and renal fibrosis are examined. In renal tissues from hyperuricemic rats, TUG1 and CtsD are upregulated, while miR-140-3p is downregulated. Inhibiting TUG1 or CtsD or upregulating miR-140-3p relieves renal fibrosis in hyperuricemic rats. Downregulated miR-140-3p reverses the therapeutic effect of TUG1 reduction, while overexpression of CtsD abolishes the role of miR-140-3p upregulation in renal fibrosis. Collectively, this study highlights that TUG1 inhibition upregulates miR-140-3p to ameliorate renal fibrosis in hyperuricemic rats by inhibiting CtsD. |
| first_indexed | 2024-03-11T12:21:02Z |
| format | Article |
| id | doaj.art-aedb42c9fcda497db3c6f78572504294 |
| institution | Directory Open Access Journal |
| issn | 1672-9145 |
| language | English |
| last_indexed | 2024-03-11T12:21:02Z |
| publishDate | 2022-09-01 |
| publisher | China Science Publishing & Media Ltd. |
| record_format | Article |
| series | Acta Biochimica et Biophysica Sinica |
| spelling | doaj.art-aedb42c9fcda497db3c6f785725042942023-11-07T01:01:41ZengChina Science Publishing & Media Ltd.Acta Biochimica et Biophysica Sinica1672-91452022-09-01541365137510.3724/abbs.202212820d259cclncRNA TUG1 regulates hyperuricemia-induced renal <?A3B2 pi6?>fibrosis in a rat modelZhang Ying0Zhang Haizhen1Hu Langtao2Wei Jiali3Ma Chunyang4["Department of Nephrology, Hainan General Hospital (Hainan Affiliated Hospital of Hainan Medical University), Haikou 570311, China"]["Department of Nephrology, Hainan General Hospital (Hainan Affiliated Hospital of Hainan Medical University), Haikou 570311, China"]["Department of Nephrology, Hainan General Hospital (Hainan Affiliated Hospital of Hainan Medical University), Haikou 570311, China"]["Department of Nephrology, Hainan General Hospital (Hainan Affiliated Hospital of Hainan Medical University), Haikou 570311, China"]["of Neurosurgery, First Affiliated Hospital of Hainan Medical University, Haikou 570100, China"]Renal fibrosis is most common among chronic kidney diseases. Molecular studies have shown that long noncoding RNAs (lncRNAs) and microRNAs (miRNAs) participate in renal fibrosis, while the roles of lncRNA taurine upregulated gene 1 (TUG1) and miR-140-3p in hyperuricemia-induced renal fibrosis remain less investigated. In this study, a rat hyperuricemia model is constructed by oral administration of adenine. TUG1, miR-140-3p, and cathepsin D (CtsD) expression levels in rat models are measured. After altering TUG1, miR-140-3p, or CtsD expression in modelled rats, biochemical indices, including uric acid (UA), serum creatine (SCr), blood urea nitrogen (BUN), and 24-h urine protein are detected, pathological changes in the renal tissues, and renal fibrosis are examined. In renal tissues from hyperuricemic rats, TUG1 and CtsD are upregulated, while miR-140-3p is downregulated. Inhibiting TUG1 or CtsD or upregulating miR-140-3p relieves renal fibrosis in hyperuricemic rats. Downregulated miR-140-3p reverses the therapeutic effect of TUG1 reduction, while overexpression of CtsD abolishes the role of miR-140-3p upregulation in renal fibrosis. Collectively, this study highlights that TUG1 inhibition upregulates miR-140-3p to ameliorate renal fibrosis in hyperuricemic rats by inhibiting CtsD.https://www.sciengine.com/doi/10.3724/abbs.2022128hyperuricemiarenal fibrosistaurine upregulated gene 1miR-140-3pcathepsin D |
| spellingShingle | Zhang Ying Zhang Haizhen Hu Langtao Wei Jiali Ma Chunyang lncRNA TUG1 regulates hyperuricemia-induced renal <?A3B2 pi6?>fibrosis in a rat model Acta Biochimica et Biophysica Sinica hyperuricemia renal fibrosis taurine upregulated gene 1 miR-140-3p cathepsin D |
| title | lncRNA TUG1 regulates hyperuricemia-induced renal <?A3B2 pi6?>fibrosis in a rat model |
| title_full | lncRNA TUG1 regulates hyperuricemia-induced renal <?A3B2 pi6?>fibrosis in a rat model |
| title_fullStr | lncRNA TUG1 regulates hyperuricemia-induced renal <?A3B2 pi6?>fibrosis in a rat model |
| title_full_unstemmed | lncRNA TUG1 regulates hyperuricemia-induced renal <?A3B2 pi6?>fibrosis in a rat model |
| title_short | lncRNA TUG1 regulates hyperuricemia-induced renal <?A3B2 pi6?>fibrosis in a rat model |
| title_sort | lncrna tug1 regulates hyperuricemia induced renal a3b2 pi6 fibrosis in a rat model |
| topic | hyperuricemia renal fibrosis taurine upregulated gene 1 miR-140-3p cathepsin D |
| url | https://www.sciengine.com/doi/10.3724/abbs.2022128 |
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