The accessory gene regulator (agr) controls Staphylococcus aureus virulence in a murine intracranial abscesses model
Background: Intracranial abscesses are associated with high mortality. Staphylococcus aureus is one of the main pathogens that cause intracranial infection. Until now, there is no report to identify the key effectors of S. aureus during the intracranial infection. Methods: The murine intracranial ab...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2014-09-01
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| Series: | Brazilian Journal of Infectious Diseases |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1413867014000932 |
| _version_ | 1818398999398318080 |
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| author | Jian Gong Dongzhi Li Jun Yan Yu Liu Di Li Jie Dong Yaping Gao Tao Sun Guang Yang |
| author_facet | Jian Gong Dongzhi Li Jun Yan Yu Liu Di Li Jie Dong Yaping Gao Tao Sun Guang Yang |
| author_sort | Jian Gong |
| collection | DOAJ |
| description | Background: Intracranial abscesses are associated with high mortality. Staphylococcus aureus is one of the main pathogens that cause intracranial infection. Until now, there is no report to identify the key effectors of S. aureus during the intracranial infection. Methods: The murine intracranial abscesses model induced by S. aureus was constructed. The vital sign and survival rate of mice were observed to evaluate the infection. Histological examination was used to diagnose the pathological alterations of mouse tissues. The sensitivity of S. aureus to whole blood was evaluated by whole-blood killing assay. Results: In murine intracranial abscesses model, it was shown that the mortality caused by the accessory gene regulator (agr) locus deficient strain was significant decreased compared with its parent strain. Moreover, we found that RNAIII, the effector of agr system, was essential for the intracranial infection caused by S. aureus. In the further investigation, it was shown that restoration the expression of α-toxin in agr deficient strain could partially recover the mortality in the murine intracranial abscesses model. Conclusion: Our data suggested that the agr system of S. aureus is an important virulence determinant in the induction and mortality of intracranial abscesses in mice. Keywords: S. aureus, Agr, Intracranial abscesses model |
| first_indexed | 2024-12-14T07:13:42Z |
| format | Article |
| id | doaj.art-aee0b385766e403fa4bb626335a0c311 |
| institution | Directory Open Access Journal |
| issn | 1413-8670 |
| language | English |
| last_indexed | 2024-12-14T07:13:42Z |
| publishDate | 2014-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Infectious Diseases |
| spelling | doaj.art-aee0b385766e403fa4bb626335a0c3112022-12-21T23:11:45ZengElsevierBrazilian Journal of Infectious Diseases1413-86702014-09-01185501506S1413-86702014000500501The accessory gene regulator (agr) controls Staphylococcus aureus virulence in a murine intracranial abscesses modelJian Gong0Dongzhi Li1Jun Yan2Yu Liu3Di Li4Jie Dong5Yaping Gao6Tao Sun7Guang Yang8Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing Neurosurgical Institute, Beijing, ChinaNingxia Medical University, Incubation Base of National Key Laboratory for Cerebrocranial Diseases, Yinchuan, ChinaBeijing Institute of Basic Medical Sciences, Beijing, ChinaBeijing Institute of Basic Medical Sciences, Beijing, ChinaBeijing Institute of Basic Medical Sciences, Beijing, ChinaBeijing Institute of Basic Medical Sciences, Beijing, ChinaBeijing Institute of Basic Medical Sciences, Beijing, ChinaNingxia Medical University, Incubation Base of National Key Laboratory for Cerebrocranial Diseases, Yinchuan, China; Co-corresponding author.Beijing Institute of Basic Medical Sciences, Beijing, China; Corresponding author at: Institute of Basic Medical Sciences, 27 Taiping Road, Beijing 100850, China.; Co-corresponding author.Background: Intracranial abscesses are associated with high mortality. Staphylococcus aureus is one of the main pathogens that cause intracranial infection. Until now, there is no report to identify the key effectors of S. aureus during the intracranial infection. Methods: The murine intracranial abscesses model induced by S. aureus was constructed. The vital sign and survival rate of mice were observed to evaluate the infection. Histological examination was used to diagnose the pathological alterations of mouse tissues. The sensitivity of S. aureus to whole blood was evaluated by whole-blood killing assay. Results: In murine intracranial abscesses model, it was shown that the mortality caused by the accessory gene regulator (agr) locus deficient strain was significant decreased compared with its parent strain. Moreover, we found that RNAIII, the effector of agr system, was essential for the intracranial infection caused by S. aureus. In the further investigation, it was shown that restoration the expression of α-toxin in agr deficient strain could partially recover the mortality in the murine intracranial abscesses model. Conclusion: Our data suggested that the agr system of S. aureus is an important virulence determinant in the induction and mortality of intracranial abscesses in mice. Keywords: S. aureus, Agr, Intracranial abscesses modelhttp://www.sciencedirect.com/science/article/pii/S1413867014000932 |
| spellingShingle | Jian Gong Dongzhi Li Jun Yan Yu Liu Di Li Jie Dong Yaping Gao Tao Sun Guang Yang The accessory gene regulator (agr) controls Staphylococcus aureus virulence in a murine intracranial abscesses model Brazilian Journal of Infectious Diseases |
| title | The accessory gene regulator (agr) controls Staphylococcus aureus virulence in a murine intracranial abscesses model |
| title_full | The accessory gene regulator (agr) controls Staphylococcus aureus virulence in a murine intracranial abscesses model |
| title_fullStr | The accessory gene regulator (agr) controls Staphylococcus aureus virulence in a murine intracranial abscesses model |
| title_full_unstemmed | The accessory gene regulator (agr) controls Staphylococcus aureus virulence in a murine intracranial abscesses model |
| title_short | The accessory gene regulator (agr) controls Staphylococcus aureus virulence in a murine intracranial abscesses model |
| title_sort | accessory gene regulator agr controls staphylococcus aureus virulence in a murine intracranial abscesses model |
| url | http://www.sciencedirect.com/science/article/pii/S1413867014000932 |
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