Genome-wide CRISPR-KO Screen Uncovers mTORC1-Mediated Gsk3 Regulation in Naive Pluripotency Maintenance and Dissolution

Genome-wide CRISPR-KO Screen Uncovers mTORC1-Mediated Gsk3 Regulation in Naive Pluripotency Maintenance and Dissolution

Summary: The genetic basis of naive pluripotency maintenance and loss is a central question in embryonic stem cell biology. Here, we deploy CRISPR-knockout-based screens in mouse embryonic stem cells to interrogate this question through a genome-wide, non-biased approach using the Rex1GFP reporter a...

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Bibliographic Details
Main Authors: Meng Li, Jason S.L. Yu, Katarzyna Tilgner, Swee Hoe Ong, Hiroko Koike-Yusa, Kosuke Yusa
Format: Article
Language:English
Published: Elsevier 2018-07-01
Series:Cell Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124718309227

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