Neobalantidium coli: First molecular identification from the Eurasian wild boar, Sus Scrofa in Bushehr Province, Southwestern Iran

Abstract Balantidium coli is a common parasite of pig and wild boars (Sus scrofa) which can infect humans and several species of mammals. This study aimed to determine the genotype of Balantidium isolated from Eurasian wild boars in Bushehr province, Southwestern Iran. Twenty‐five faecal samples, or...

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Main Authors: Shamsi Noorpisheh Ghadimi, Mohammad R. Abedini, Bahador Sarkari, Amir Savardashtaki, Fattaneh Mikaeili
Format: Article
Language:English
Published: Wiley 2020-02-01
Series:Veterinary Medicine and Science
Subjects:
Online Access:https://doi.org/10.1002/vms3.210
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author Shamsi Noorpisheh Ghadimi
Mohammad R. Abedini
Bahador Sarkari
Amir Savardashtaki
Fattaneh Mikaeili
author_facet Shamsi Noorpisheh Ghadimi
Mohammad R. Abedini
Bahador Sarkari
Amir Savardashtaki
Fattaneh Mikaeili
author_sort Shamsi Noorpisheh Ghadimi
collection DOAJ
description Abstract Balantidium coli is a common parasite of pig and wild boars (Sus scrofa) which can infect humans and several species of mammals. This study aimed to determine the genotype of Balantidium isolated from Eurasian wild boars in Bushehr province, Southwestern Iran. Twenty‐five faecal samples, originating from 25 wild boars captivated in our previous study, were processed. DNA was extracted from the faecal samples and PCR‐amplified, targeting an ITS1–5.8s‐rRNA–ITS2 region of Balantidium genome. PCR product was purified from the gel, and sequenced. BLAST analysis was performed in order to compare our isolates with other previously reported ones. A phylogenetic tree was constructed, using MegaX software, to find out the phylogenetic diversity of the isolates. With PCR it was possible to detect Balantidium DNA in the faecal samples of 13 out of 25 (52%) of the wild boars. BLAST analysis of seven isolates revealed that the isolates belong to the newly introduced genus Neobalantidium coli. Sequences of three isolates were deposited in the GenBank. Moreover, molecular analysis revealed six areas of nucleotide differences within the isolates and nine areas of difference between the sequences obtained in this study and those available in the GenBank. Phylogenetic analysis revealed that the sequences of isolates of this study have up to 2.2% dissimilarity from those published in the GenBank. The findings of this study, for the first time, revealed that some of the isolates of Balantidium originating from wild boars in Southwestern Iran belonged to the N. coli.
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spelling doaj.art-aee3ac0d65fa4c46b149fa312d250bfa2022-12-21T18:53:10ZengWileyVeterinary Medicine and Science2053-10952020-02-016114214610.1002/vms3.210Neobalantidium coli: First molecular identification from the Eurasian wild boar, Sus Scrofa in Bushehr Province, Southwestern IranShamsi Noorpisheh Ghadimi0Mohammad R. Abedini1Bahador Sarkari2Amir Savardashtaki3Fattaneh Mikaeili4Department of Parasitology and Mycology School of Medicine Shiraz University of Medical Sciences Shiraz IranDepartment of Parasitology and Mycology School of Medicine Shiraz University of Medical Sciences Shiraz IranDepartment of Parasitology and Mycology School of Medicine Shiraz University of Medical Sciences Shiraz IranDepartment of Medical Biotechnology School of Advanced Medical Sciences and Technologies Shiraz University of Medical Sciences Shiraz IranDepartment of Parasitology and Mycology School of Medicine Shiraz University of Medical Sciences Shiraz IranAbstract Balantidium coli is a common parasite of pig and wild boars (Sus scrofa) which can infect humans and several species of mammals. This study aimed to determine the genotype of Balantidium isolated from Eurasian wild boars in Bushehr province, Southwestern Iran. Twenty‐five faecal samples, originating from 25 wild boars captivated in our previous study, were processed. DNA was extracted from the faecal samples and PCR‐amplified, targeting an ITS1–5.8s‐rRNA–ITS2 region of Balantidium genome. PCR product was purified from the gel, and sequenced. BLAST analysis was performed in order to compare our isolates with other previously reported ones. A phylogenetic tree was constructed, using MegaX software, to find out the phylogenetic diversity of the isolates. With PCR it was possible to detect Balantidium DNA in the faecal samples of 13 out of 25 (52%) of the wild boars. BLAST analysis of seven isolates revealed that the isolates belong to the newly introduced genus Neobalantidium coli. Sequences of three isolates were deposited in the GenBank. Moreover, molecular analysis revealed six areas of nucleotide differences within the isolates and nine areas of difference between the sequences obtained in this study and those available in the GenBank. Phylogenetic analysis revealed that the sequences of isolates of this study have up to 2.2% dissimilarity from those published in the GenBank. The findings of this study, for the first time, revealed that some of the isolates of Balantidium originating from wild boars in Southwestern Iran belonged to the N. coli.https://doi.org/10.1002/vms3.210genetic diversitygenotypeIranNeobalantidiumSus scrofawild boars
spellingShingle Shamsi Noorpisheh Ghadimi
Mohammad R. Abedini
Bahador Sarkari
Amir Savardashtaki
Fattaneh Mikaeili
Neobalantidium coli: First molecular identification from the Eurasian wild boar, Sus Scrofa in Bushehr Province, Southwestern Iran
Veterinary Medicine and Science
genetic diversity
genotype
Iran
Neobalantidium
Sus scrofa
wild boars
title Neobalantidium coli: First molecular identification from the Eurasian wild boar, Sus Scrofa in Bushehr Province, Southwestern Iran
title_full Neobalantidium coli: First molecular identification from the Eurasian wild boar, Sus Scrofa in Bushehr Province, Southwestern Iran
title_fullStr Neobalantidium coli: First molecular identification from the Eurasian wild boar, Sus Scrofa in Bushehr Province, Southwestern Iran
title_full_unstemmed Neobalantidium coli: First molecular identification from the Eurasian wild boar, Sus Scrofa in Bushehr Province, Southwestern Iran
title_short Neobalantidium coli: First molecular identification from the Eurasian wild boar, Sus Scrofa in Bushehr Province, Southwestern Iran
title_sort neobalantidium coli first molecular identification from the eurasian wild boar sus scrofa in bushehr province southwestern iran
topic genetic diversity
genotype
Iran
Neobalantidium
Sus scrofa
wild boars
url https://doi.org/10.1002/vms3.210
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