Neobalantidium coli: First molecular identification from the Eurasian wild boar, Sus Scrofa in Bushehr Province, Southwestern Iran
Abstract Balantidium coli is a common parasite of pig and wild boars (Sus scrofa) which can infect humans and several species of mammals. This study aimed to determine the genotype of Balantidium isolated from Eurasian wild boars in Bushehr province, Southwestern Iran. Twenty‐five faecal samples, or...
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Wiley
2020-02-01
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Series: | Veterinary Medicine and Science |
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Online Access: | https://doi.org/10.1002/vms3.210 |
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author | Shamsi Noorpisheh Ghadimi Mohammad R. Abedini Bahador Sarkari Amir Savardashtaki Fattaneh Mikaeili |
author_facet | Shamsi Noorpisheh Ghadimi Mohammad R. Abedini Bahador Sarkari Amir Savardashtaki Fattaneh Mikaeili |
author_sort | Shamsi Noorpisheh Ghadimi |
collection | DOAJ |
description | Abstract Balantidium coli is a common parasite of pig and wild boars (Sus scrofa) which can infect humans and several species of mammals. This study aimed to determine the genotype of Balantidium isolated from Eurasian wild boars in Bushehr province, Southwestern Iran. Twenty‐five faecal samples, originating from 25 wild boars captivated in our previous study, were processed. DNA was extracted from the faecal samples and PCR‐amplified, targeting an ITS1–5.8s‐rRNA–ITS2 region of Balantidium genome. PCR product was purified from the gel, and sequenced. BLAST analysis was performed in order to compare our isolates with other previously reported ones. A phylogenetic tree was constructed, using MegaX software, to find out the phylogenetic diversity of the isolates. With PCR it was possible to detect Balantidium DNA in the faecal samples of 13 out of 25 (52%) of the wild boars. BLAST analysis of seven isolates revealed that the isolates belong to the newly introduced genus Neobalantidium coli. Sequences of three isolates were deposited in the GenBank. Moreover, molecular analysis revealed six areas of nucleotide differences within the isolates and nine areas of difference between the sequences obtained in this study and those available in the GenBank. Phylogenetic analysis revealed that the sequences of isolates of this study have up to 2.2% dissimilarity from those published in the GenBank. The findings of this study, for the first time, revealed that some of the isolates of Balantidium originating from wild boars in Southwestern Iran belonged to the N. coli. |
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issn | 2053-1095 |
language | English |
last_indexed | 2024-12-21T19:12:30Z |
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spelling | doaj.art-aee3ac0d65fa4c46b149fa312d250bfa2022-12-21T18:53:10ZengWileyVeterinary Medicine and Science2053-10952020-02-016114214610.1002/vms3.210Neobalantidium coli: First molecular identification from the Eurasian wild boar, Sus Scrofa in Bushehr Province, Southwestern IranShamsi Noorpisheh Ghadimi0Mohammad R. Abedini1Bahador Sarkari2Amir Savardashtaki3Fattaneh Mikaeili4Department of Parasitology and Mycology School of Medicine Shiraz University of Medical Sciences Shiraz IranDepartment of Parasitology and Mycology School of Medicine Shiraz University of Medical Sciences Shiraz IranDepartment of Parasitology and Mycology School of Medicine Shiraz University of Medical Sciences Shiraz IranDepartment of Medical Biotechnology School of Advanced Medical Sciences and Technologies Shiraz University of Medical Sciences Shiraz IranDepartment of Parasitology and Mycology School of Medicine Shiraz University of Medical Sciences Shiraz IranAbstract Balantidium coli is a common parasite of pig and wild boars (Sus scrofa) which can infect humans and several species of mammals. This study aimed to determine the genotype of Balantidium isolated from Eurasian wild boars in Bushehr province, Southwestern Iran. Twenty‐five faecal samples, originating from 25 wild boars captivated in our previous study, were processed. DNA was extracted from the faecal samples and PCR‐amplified, targeting an ITS1–5.8s‐rRNA–ITS2 region of Balantidium genome. PCR product was purified from the gel, and sequenced. BLAST analysis was performed in order to compare our isolates with other previously reported ones. A phylogenetic tree was constructed, using MegaX software, to find out the phylogenetic diversity of the isolates. With PCR it was possible to detect Balantidium DNA in the faecal samples of 13 out of 25 (52%) of the wild boars. BLAST analysis of seven isolates revealed that the isolates belong to the newly introduced genus Neobalantidium coli. Sequences of three isolates were deposited in the GenBank. Moreover, molecular analysis revealed six areas of nucleotide differences within the isolates and nine areas of difference between the sequences obtained in this study and those available in the GenBank. Phylogenetic analysis revealed that the sequences of isolates of this study have up to 2.2% dissimilarity from those published in the GenBank. The findings of this study, for the first time, revealed that some of the isolates of Balantidium originating from wild boars in Southwestern Iran belonged to the N. coli.https://doi.org/10.1002/vms3.210genetic diversitygenotypeIranNeobalantidiumSus scrofawild boars |
spellingShingle | Shamsi Noorpisheh Ghadimi Mohammad R. Abedini Bahador Sarkari Amir Savardashtaki Fattaneh Mikaeili Neobalantidium coli: First molecular identification from the Eurasian wild boar, Sus Scrofa in Bushehr Province, Southwestern Iran Veterinary Medicine and Science genetic diversity genotype Iran Neobalantidium Sus scrofa wild boars |
title | Neobalantidium coli: First molecular identification from the Eurasian wild boar, Sus Scrofa in Bushehr Province, Southwestern Iran |
title_full | Neobalantidium coli: First molecular identification from the Eurasian wild boar, Sus Scrofa in Bushehr Province, Southwestern Iran |
title_fullStr | Neobalantidium coli: First molecular identification from the Eurasian wild boar, Sus Scrofa in Bushehr Province, Southwestern Iran |
title_full_unstemmed | Neobalantidium coli: First molecular identification from the Eurasian wild boar, Sus Scrofa in Bushehr Province, Southwestern Iran |
title_short | Neobalantidium coli: First molecular identification from the Eurasian wild boar, Sus Scrofa in Bushehr Province, Southwestern Iran |
title_sort | neobalantidium coli first molecular identification from the eurasian wild boar sus scrofa in bushehr province southwestern iran |
topic | genetic diversity genotype Iran Neobalantidium Sus scrofa wild boars |
url | https://doi.org/10.1002/vms3.210 |
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