Role of Bleach Concentration Method for Detection of Acid-fast Bacilli (AFB) in Sputum using Conventional Ziehl-Neelsen (ZN) Staining Technique
Sputum microscopy is the most important tool for the detection of Mycobacterium tuberculosis in peripheral laboratories. Sodium hypochlorite concentration technique prior to sputum microscopy may improve the detection of AFB bacilli over direct Ziehl Neelsen’s staining method. A prospective study wa...
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| Format: | Article |
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Journal of Pure and Applied Microbiology
2023-03-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/role-of-bleach-concentration-method-for-detection-of-acid-fast-bacilli-afb-in-sputum-using-conventional-ziehl-neelsen-zn-staining-technique/ |
| _version_ | 1797868093539614720 |
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| author | Priyanka Pal Vishnu Prasad Shenoy Promukh Bhattacharya |
| author_facet | Priyanka Pal Vishnu Prasad Shenoy Promukh Bhattacharya |
| author_sort | Priyanka Pal |
| collection | DOAJ |
| description | Sputum microscopy is the most important tool for the detection of Mycobacterium tuberculosis in peripheral laboratories. Sodium hypochlorite concentration technique prior to sputum microscopy may improve the detection of AFB bacilli over direct Ziehl Neelsen’s staining method. A prospective study was conducted at Kasturba Medical College, Manipal. Consecutive sputum samples were collected for 3 months. ZN stained smears were made directly of fresh specimens and of specimens that were processed with 3.5% sodium hypochlorite (NaOCl), from each patient. Then, ZN stained smears were observed under light microscopy. A total of 239 samples were collected. The yield of positive smears with ZN staining after NaOCl centrifugation was higher than that with microscopy after direct ZN stain. The percentage increase yield of ZN staining after NaOCl centrifugation over direct ZN staining was 3.3% (8/239). Bleach centrifugation can help in increasing the yield of positivity in sputum smear. |
| first_indexed | 2024-04-09T23:51:42Z |
| format | Article |
| id | doaj.art-aee6179e748d40ca888e3dbadbe5fea0 |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-04-09T23:51:42Z |
| publishDate | 2023-03-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-aee6179e748d40ca888e3dbadbe5fea02023-03-17T09:41:43ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2023-03-01171127131https://doi.org/10.22207/JPAM.17.1.01Role of Bleach Concentration Method for Detection of Acid-fast Bacilli (AFB) in Sputum using Conventional Ziehl-Neelsen (ZN) Staining TechniquePriyanka PalVishnu Prasad Shenoyhttps://orcid.org/0000-0002-4371-1326Promukh BhattacharyaSputum microscopy is the most important tool for the detection of Mycobacterium tuberculosis in peripheral laboratories. Sodium hypochlorite concentration technique prior to sputum microscopy may improve the detection of AFB bacilli over direct Ziehl Neelsen’s staining method. A prospective study was conducted at Kasturba Medical College, Manipal. Consecutive sputum samples were collected for 3 months. ZN stained smears were made directly of fresh specimens and of specimens that were processed with 3.5% sodium hypochlorite (NaOCl), from each patient. Then, ZN stained smears were observed under light microscopy. A total of 239 samples were collected. The yield of positive smears with ZN staining after NaOCl centrifugation was higher than that with microscopy after direct ZN stain. The percentage increase yield of ZN staining after NaOCl centrifugation over direct ZN staining was 3.3% (8/239). Bleach centrifugation can help in increasing the yield of positivity in sputum smear.https://microbiologyjournal.org/role-of-bleach-concentration-method-for-detection-of-acid-fast-bacilli-afb-in-sputum-using-conventional-ziehl-neelsen-zn-staining-technique/ziehl-neelsen stainingsputummycobacterium tuberculosissodium hypochlorite |
| spellingShingle | Priyanka Pal Vishnu Prasad Shenoy Promukh Bhattacharya Role of Bleach Concentration Method for Detection of Acid-fast Bacilli (AFB) in Sputum using Conventional Ziehl-Neelsen (ZN) Staining Technique Journal of Pure and Applied Microbiology ziehl-neelsen staining sputum mycobacterium tuberculosis sodium hypochlorite |
| title | Role of Bleach Concentration Method for Detection of Acid-fast Bacilli (AFB) in Sputum using Conventional Ziehl-Neelsen (ZN) Staining Technique |
| title_full | Role of Bleach Concentration Method for Detection of Acid-fast Bacilli (AFB) in Sputum using Conventional Ziehl-Neelsen (ZN) Staining Technique |
| title_fullStr | Role of Bleach Concentration Method for Detection of Acid-fast Bacilli (AFB) in Sputum using Conventional Ziehl-Neelsen (ZN) Staining Technique |
| title_full_unstemmed | Role of Bleach Concentration Method for Detection of Acid-fast Bacilli (AFB) in Sputum using Conventional Ziehl-Neelsen (ZN) Staining Technique |
| title_short | Role of Bleach Concentration Method for Detection of Acid-fast Bacilli (AFB) in Sputum using Conventional Ziehl-Neelsen (ZN) Staining Technique |
| title_sort | role of bleach concentration method for detection of acid fast bacilli afb in sputum using conventional ziehl neelsen zn staining technique |
| topic | ziehl-neelsen staining sputum mycobacterium tuberculosis sodium hypochlorite |
| url | https://microbiologyjournal.org/role-of-bleach-concentration-method-for-detection-of-acid-fast-bacilli-afb-in-sputum-using-conventional-ziehl-neelsen-zn-staining-technique/ |
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