Comparative and Systematic Omics Revealed Low Cd Accumulation of Potato <i>StMTP</i><i>9</i> in Yeast: Suggesting a New Mechanism for Heavy Metal Detoxification
The metal tolerance protein (MTP) family is a very old family with evolutionary conservation and less specific amplification. It seems to retain the original functions of the ancestral genes and plays an important role in maintaining metal homeostasis in plant cells. We identified the potato <i&g...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-09-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/22/19/10478 |
| Summary: | The metal tolerance protein (MTP) family is a very old family with evolutionary conservation and less specific amplification. It seems to retain the original functions of the ancestral genes and plays an important role in maintaining metal homeostasis in plant cells. We identified the potato <i>MTP</i> family members for the first time, the specific and conservative <i>StMPT</i>s were discovered by using systematic and comparative omics. To be surprised, members of the <i>StMTP</i> family seem to have mutated before the evolution of dicotyledon and monocotyledon, and even the loss of the entire subfamily (subfamily G6, G7). Interestingly, <i>StMTP9</i> represents the conserved structure of the entire subfamily involved in toxic metal regulation. However, the gene structure and transmembrane domain of <i>StMTP8</i> have undergone specific evolution, showing that the transmembrane domain (Motif13) located at the NH<sub>2</sub> terminal has been replaced by the signal peptide domain, so it was selected as the control gene of <i>StMTP9</i>. Through real-time fluorescence quantitative analysis of <i>StMTP</i>s under Cd and Zn stress, a co-expression network was constructed, and it was found that <i>StMTP9</i> responded significantly to Cd stress, while <i>StMTP8</i> did the opposite. What excites us is that by introducing <i>StMTPs 8/9</i> into the <i>∆ycf1</i> yeast cadmium-sensitive mutant strain, the functional complementation experiment proved that <i>StMTPs 8/9</i> can restore Cd tolerance. In particular, <i>StMTP9</i> can greatly reduce the cadmium content in yeast cells, while <i>StMTP8</i> cannot. These findings provide a reference for further research on the molecular mechanism of potato toxic metal accumulation. |
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| ISSN: | 1661-6596 1422-0067 |