CEACAM expression in an in-vitro prostatitis model
BackgroundProstatitis is an inflammatory disease of the prostate gland, which affects 2-16% of men worldwide and thought to be a cause for prostate cancer (PCa) development. Carcinoembryogenic antigen-related cell adhesion molecules (CEACAMs) are deregulated in inflammation and in PCa. The role of C...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-08-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1236343/full |
| _version_ | 1797737039141011456 |
|---|---|
| author | Irina Kube-Golovin Mykola Lyndin Mykola Lyndin Marc Wiesehöfer Gunther Wennemuth |
| author_facet | Irina Kube-Golovin Mykola Lyndin Mykola Lyndin Marc Wiesehöfer Gunther Wennemuth |
| author_sort | Irina Kube-Golovin |
| collection | DOAJ |
| description | BackgroundProstatitis is an inflammatory disease of the prostate gland, which affects 2-16% of men worldwide and thought to be a cause for prostate cancer (PCa) development. Carcinoembryogenic antigen-related cell adhesion molecules (CEACAMs) are deregulated in inflammation and in PCa. The role of CEACAMs in prostate inflammation and their possible contribution to the malignant transformation of prostate epithelial cells is still elusive. In this study, we investigated the expression of CEACAMs in an in-vitro prostatitis model and their potential role in malignant transformation of prostate epithelial cells.MethodsNormal prostate epithelial RWPE-1 cells were treated with pro-inflammatory cytokines to achieve an inflammatory state of the cells. The expression of CEACAMs and their related isoforms were analyzed. Additionally, the expression levels of selected CEACAMs were correlated with the expression of malignancy markers and the migratory properties of the cells.ResultsThis study demonstrates that the pro-inflammatory cytokines, tumor necrosis factor alpha (TNFα) and interferon-gamma (IFNγ), induce synergistically an up-regulation of CEACAM1 expression in RWPE-1 cells, specifically favoring the CEACAM1-L isoform. Furthermore, overexpressed CEACAM1-L is associated with the deregulated expression of JAK/STAT, NFκB, and epithelial-mesenchymal transition (EMT) genes, as well as an increased cell migration.ConclusionWe postulate that CEACAM1 isoform CEACAM1-4L may synergistically contribute to inflammation-induced oncogenesis in the prostate. |
| first_indexed | 2024-03-12T13:21:38Z |
| format | Article |
| id | doaj.art-aee781a8544046ada41c40fee6009caa |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-12T13:21:38Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-aee781a8544046ada41c40fee6009caa2023-08-25T18:25:41ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-08-011410.3389/fimmu.2023.12363431236343CEACAM expression in an in-vitro prostatitis modelIrina Kube-Golovin0Mykola Lyndin1Mykola Lyndin2Marc Wiesehöfer3Gunther Wennemuth4University Hospital Essen, Department of Anatomy, Essen, GermanyUniversity Hospital Essen, Department of Anatomy, Essen, GermanyAcademic and Research Medical Institute, Department of Pathology, Sumy State University, Sumy, UkraineUniversity Hospital Essen, Department of Anatomy, Essen, GermanyUniversity Hospital Essen, Department of Anatomy, Essen, GermanyBackgroundProstatitis is an inflammatory disease of the prostate gland, which affects 2-16% of men worldwide and thought to be a cause for prostate cancer (PCa) development. Carcinoembryogenic antigen-related cell adhesion molecules (CEACAMs) are deregulated in inflammation and in PCa. The role of CEACAMs in prostate inflammation and their possible contribution to the malignant transformation of prostate epithelial cells is still elusive. In this study, we investigated the expression of CEACAMs in an in-vitro prostatitis model and their potential role in malignant transformation of prostate epithelial cells.MethodsNormal prostate epithelial RWPE-1 cells were treated with pro-inflammatory cytokines to achieve an inflammatory state of the cells. The expression of CEACAMs and their related isoforms were analyzed. Additionally, the expression levels of selected CEACAMs were correlated with the expression of malignancy markers and the migratory properties of the cells.ResultsThis study demonstrates that the pro-inflammatory cytokines, tumor necrosis factor alpha (TNFα) and interferon-gamma (IFNγ), induce synergistically an up-regulation of CEACAM1 expression in RWPE-1 cells, specifically favoring the CEACAM1-L isoform. Furthermore, overexpressed CEACAM1-L is associated with the deregulated expression of JAK/STAT, NFκB, and epithelial-mesenchymal transition (EMT) genes, as well as an increased cell migration.ConclusionWe postulate that CEACAM1 isoform CEACAM1-4L may synergistically contribute to inflammation-induced oncogenesis in the prostate.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1236343/fullprostatitisinflammationCEACAMCEACAM1prostate cancer |
| spellingShingle | Irina Kube-Golovin Mykola Lyndin Mykola Lyndin Marc Wiesehöfer Gunther Wennemuth CEACAM expression in an in-vitro prostatitis model Frontiers in Immunology prostatitis inflammation CEACAM CEACAM1 prostate cancer |
| title | CEACAM expression in an in-vitro prostatitis model |
| title_full | CEACAM expression in an in-vitro prostatitis model |
| title_fullStr | CEACAM expression in an in-vitro prostatitis model |
| title_full_unstemmed | CEACAM expression in an in-vitro prostatitis model |
| title_short | CEACAM expression in an in-vitro prostatitis model |
| title_sort | ceacam expression in an in vitro prostatitis model |
| topic | prostatitis inflammation CEACAM CEACAM1 prostate cancer |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1236343/full |
| work_keys_str_mv | AT irinakubegolovin ceacamexpressioninaninvitroprostatitismodel AT mykolalyndin ceacamexpressioninaninvitroprostatitismodel AT mykolalyndin ceacamexpressioninaninvitroprostatitismodel AT marcwiesehofer ceacamexpressioninaninvitroprostatitismodel AT guntherwennemuth ceacamexpressioninaninvitroprostatitismodel |