<i>Moringa oleifera</i> Leaf Extract Protects C2C12 Myotubes against H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress
The imbalance between reactive oxygen species (ROS) production and antioxidant defense systems leads to macromolecule and tissue damage as a result of cellular oxidative stress. This phenomenon is considered a key factor in fatigue and muscle damage following chronic or high-intensity physical exerc...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/8/1435 |
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| author | Roberta Ceci Mariateresa Maldini Mark E. Olson Domenico Crognale Katy Horner Ivan Dimauro Stefania Sabatini Guglielmo Duranti |
| author_facet | Roberta Ceci Mariateresa Maldini Mark E. Olson Domenico Crognale Katy Horner Ivan Dimauro Stefania Sabatini Guglielmo Duranti |
| author_sort | Roberta Ceci |
| collection | DOAJ |
| description | The imbalance between reactive oxygen species (ROS) production and antioxidant defense systems leads to macromolecule and tissue damage as a result of cellular oxidative stress. This phenomenon is considered a key factor in fatigue and muscle damage following chronic or high-intensity physical exercise. In the present study, the antioxidant effect of <i>Moringa oleifera</i> leaf extract (MOLE) was evaluated in C2C12 myotubes exposed to an elevated hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) insult. The capacity of the extract to influence the myotube redox status was evaluated through an analysis of the total antioxidant capacity (TAC), glutathione homeostasis (GSH and GSSG), total free thiols (TFT), and thioredoxin (Trx) activity, as well as the enzyme activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) and transferase (GST). Moreover, the ability of MOLE to mitigate the stress-induced peroxidation of lipids and oxidative damage (TBARS and protein carbonyls) was also evaluated. Our data demonstrate that MOLE pre-treatment mitigates the highly stressful effects of H<sub>2</sub>O<sub>2</sub> in myotubes (1 mM) by restoring the redox status (TFT, Trx, and GSH/GSSG ratio) and increasing the antioxidant enzymatic system (CAT, SOD, GPx, GST), thereby significantly reducing the TBARs and PrCAR levels. Our study provides evidence that MOLE supplementation has antioxidant potential, allowing myotubes better able to cope with an oxidative insult and, therefore, could represent a useful nutritional strategy for the preservation of muscle well-being. |
| first_indexed | 2024-03-09T11:59:52Z |
| format | Article |
| id | doaj.art-aee824e13fbb47dca4321201129e7be4 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T11:59:52Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-aee824e13fbb47dca4321201129e7be42023-11-30T23:05:29ZengMDPI AGAntioxidants2076-39212022-07-01118143510.3390/antiox11081435<i>Moringa oleifera</i> Leaf Extract Protects C2C12 Myotubes against H<sub>2</sub>O<sub>2</sub>-Induced Oxidative StressRoberta Ceci0Mariateresa Maldini1Mark E. Olson2Domenico Crognale3Katy Horner4Ivan Dimauro5Stefania Sabatini6Guglielmo Duranti7Laboratory of Biochemistry and Molecular Biology—Department of Movement, Human and Health Sciences, Università degli Studi di Roma “Foro Italico”, Piazza Lauro De Bosis 6, 00135 Roma, ItalySCIEX Italia S.r.l., Via Montenapoleone, 8, 20121 Milano, ItalyInstituto de Biología, Universidad Nacional Autónoma de México, Tercer Circuito de CU s/n, Ciudad de México 04510, MexicoInstitute for Sport & Health, School of Public Health, Physiotherapy and Sports Science, University College Dublin, D04 V1W8 Dublin, IrelandInstitute for Sport & Health, School of Public Health, Physiotherapy and Sports Science, University College Dublin, D04 V1W8 Dublin, IrelandLaboratory of Biology and Human Genetics—Department of Movement, Human and Health Sciences, Università degli Studi di Roma “Foro Italico”, Piazza Lauro De Bosis 6, 00135 Roma, ItalyLaboratory of Biochemistry and Molecular Biology—Department of Movement, Human and Health Sciences, Università degli Studi di Roma “Foro Italico”, Piazza Lauro De Bosis 6, 00135 Roma, ItalyLaboratory of Biochemistry and Molecular Biology—Department of Movement, Human and Health Sciences, Università degli Studi di Roma “Foro Italico”, Piazza Lauro De Bosis 6, 00135 Roma, ItalyThe imbalance between reactive oxygen species (ROS) production and antioxidant defense systems leads to macromolecule and tissue damage as a result of cellular oxidative stress. This phenomenon is considered a key factor in fatigue and muscle damage following chronic or high-intensity physical exercise. In the present study, the antioxidant effect of <i>Moringa oleifera</i> leaf extract (MOLE) was evaluated in C2C12 myotubes exposed to an elevated hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) insult. The capacity of the extract to influence the myotube redox status was evaluated through an analysis of the total antioxidant capacity (TAC), glutathione homeostasis (GSH and GSSG), total free thiols (TFT), and thioredoxin (Trx) activity, as well as the enzyme activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) and transferase (GST). Moreover, the ability of MOLE to mitigate the stress-induced peroxidation of lipids and oxidative damage (TBARS and protein carbonyls) was also evaluated. Our data demonstrate that MOLE pre-treatment mitigates the highly stressful effects of H<sub>2</sub>O<sub>2</sub> in myotubes (1 mM) by restoring the redox status (TFT, Trx, and GSH/GSSG ratio) and increasing the antioxidant enzymatic system (CAT, SOD, GPx, GST), thereby significantly reducing the TBARs and PrCAR levels. Our study provides evidence that MOLE supplementation has antioxidant potential, allowing myotubes better able to cope with an oxidative insult and, therefore, could represent a useful nutritional strategy for the preservation of muscle well-being.https://www.mdpi.com/2076-3921/11/8/1435<i>Moringa oleifera</i> leaf extract (MOLE)C2C12 skeletal muscle cellsredox statusenzymatic antioxidant systemoxidative stress |
| spellingShingle | Roberta Ceci Mariateresa Maldini Mark E. Olson Domenico Crognale Katy Horner Ivan Dimauro Stefania Sabatini Guglielmo Duranti <i>Moringa oleifera</i> Leaf Extract Protects C2C12 Myotubes against H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress Antioxidants <i>Moringa oleifera</i> leaf extract (MOLE) C2C12 skeletal muscle cells redox status enzymatic antioxidant system oxidative stress |
| title | <i>Moringa oleifera</i> Leaf Extract Protects C2C12 Myotubes against H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress |
| title_full | <i>Moringa oleifera</i> Leaf Extract Protects C2C12 Myotubes against H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress |
| title_fullStr | <i>Moringa oleifera</i> Leaf Extract Protects C2C12 Myotubes against H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress |
| title_full_unstemmed | <i>Moringa oleifera</i> Leaf Extract Protects C2C12 Myotubes against H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress |
| title_short | <i>Moringa oleifera</i> Leaf Extract Protects C2C12 Myotubes against H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress |
| title_sort | i moringa oleifera i leaf extract protects c2c12 myotubes against h sub 2 sub o sub 2 sub induced oxidative stress |
| topic | <i>Moringa oleifera</i> leaf extract (MOLE) C2C12 skeletal muscle cells redox status enzymatic antioxidant system oxidative stress |
| url | https://www.mdpi.com/2076-3921/11/8/1435 |
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