Protein Phosphorylation Changes During Systemic Acquired Resistance in Arabidopsis thaliana
Systemic acquired resistance (SAR) in plants is a defense response that provides resistance against a wide range of pathogens at the whole-plant level following primary infection. Although the molecular mechanisms of SAR have been extensively studied in recent years, the role of phosphorylation that...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.748287/full |
| _version_ | 1818399173071863808 |
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| author | Qingfeng Zhou Qi Meng Xiaomin Tan Wei Ding Kang Ma Ziqin Xu Xuan Huang Hang Gao |
| author_facet | Qingfeng Zhou Qi Meng Xiaomin Tan Wei Ding Kang Ma Ziqin Xu Xuan Huang Hang Gao |
| author_sort | Qingfeng Zhou |
| collection | DOAJ |
| description | Systemic acquired resistance (SAR) in plants is a defense response that provides resistance against a wide range of pathogens at the whole-plant level following primary infection. Although the molecular mechanisms of SAR have been extensively studied in recent years, the role of phosphorylation that occurs in systemic leaves of SAR-induced plants is poorly understood. We used a data-independent acquisition (DIA) phosphoproteomics platform based on high-resolution mass spectrometry in an Arabidopsis thaliana model to identify phosphoproteins related to SAR establishment. A total of 8011 phosphorylation sites from 3234 proteins were identified in systemic leaves of Pseudomonas syringae pv. maculicola ES4326 (Psm ES4326) and mock locally inoculated plants. A total of 859 significantly changed phosphoproteins from 1119 significantly changed phosphopeptides were detected in systemic leaves of Psm ES4326 locally inoculated plants, including numerous transcription factors and kinases. A variety of defense response-related proteins were found to be differentially phosphorylated in systemic leaves of Psm ES4326 locally inoculated leaves, suggesting that these proteins may be functionally involved in SAR through phosphorylation or dephosphorylation. Significantly changed phosphoproteins were enriched mainly in categories related to response to abscisic acid, regulation of stomatal movement, plant–pathogen interaction, MAPK signaling pathway, purine metabolism, photosynthesis-antenna proteins, and flavonoid biosynthesis. A total of 28 proteins were regulated at both protein and phosphorylation levels during SAR. RT-qPCR analysis revealed that changes in phosphorylation levels of proteins during SAR did not result from changes in transcript abundance. This study provides comprehensive details of key phosphoproteins associated with SAR, which will facilitate further research on the molecular mechanisms of SAR. |
| first_indexed | 2024-12-14T07:16:28Z |
| format | Article |
| id | doaj.art-aee84fc5dc954e759527daa33c19ac9e |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-14T07:16:28Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-aee84fc5dc954e759527daa33c19ac9e2022-12-21T23:11:42ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-11-011210.3389/fpls.2021.748287748287Protein Phosphorylation Changes During Systemic Acquired Resistance in Arabidopsis thalianaQingfeng Zhou0Qi Meng1Xiaomin Tan2Wei Ding3Kang Ma4Ziqin Xu5Xuan Huang6Hang Gao7College of Biology and Food, Shangqiu Normal University, Shangqiu, ChinaKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi’an, ChinaKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi’an, ChinaShanghai Omicsspace Biotechnology Co., Ltd., Shanghai, ChinaCollege of Biology and Food, Shangqiu Normal University, Shangqiu, ChinaKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi’an, ChinaKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi’an, ChinaCollege of Biology and Food, Shangqiu Normal University, Shangqiu, ChinaSystemic acquired resistance (SAR) in plants is a defense response that provides resistance against a wide range of pathogens at the whole-plant level following primary infection. Although the molecular mechanisms of SAR have been extensively studied in recent years, the role of phosphorylation that occurs in systemic leaves of SAR-induced plants is poorly understood. We used a data-independent acquisition (DIA) phosphoproteomics platform based on high-resolution mass spectrometry in an Arabidopsis thaliana model to identify phosphoproteins related to SAR establishment. A total of 8011 phosphorylation sites from 3234 proteins were identified in systemic leaves of Pseudomonas syringae pv. maculicola ES4326 (Psm ES4326) and mock locally inoculated plants. A total of 859 significantly changed phosphoproteins from 1119 significantly changed phosphopeptides were detected in systemic leaves of Psm ES4326 locally inoculated plants, including numerous transcription factors and kinases. A variety of defense response-related proteins were found to be differentially phosphorylated in systemic leaves of Psm ES4326 locally inoculated leaves, suggesting that these proteins may be functionally involved in SAR through phosphorylation or dephosphorylation. Significantly changed phosphoproteins were enriched mainly in categories related to response to abscisic acid, regulation of stomatal movement, plant–pathogen interaction, MAPK signaling pathway, purine metabolism, photosynthesis-antenna proteins, and flavonoid biosynthesis. A total of 28 proteins were regulated at both protein and phosphorylation levels during SAR. RT-qPCR analysis revealed that changes in phosphorylation levels of proteins during SAR did not result from changes in transcript abundance. This study provides comprehensive details of key phosphoproteins associated with SAR, which will facilitate further research on the molecular mechanisms of SAR.https://www.frontiersin.org/articles/10.3389/fpls.2021.748287/fullphosphoproteomicsArabidopsissystemic acquired resistancedata-independent acquisitioncalcium-dependent protein kinase (CDPK)MAPK |
| spellingShingle | Qingfeng Zhou Qi Meng Xiaomin Tan Wei Ding Kang Ma Ziqin Xu Xuan Huang Hang Gao Protein Phosphorylation Changes During Systemic Acquired Resistance in Arabidopsis thaliana Frontiers in Plant Science phosphoproteomics Arabidopsis systemic acquired resistance data-independent acquisition calcium-dependent protein kinase (CDPK) MAPK |
| title | Protein Phosphorylation Changes During Systemic Acquired Resistance in Arabidopsis thaliana |
| title_full | Protein Phosphorylation Changes During Systemic Acquired Resistance in Arabidopsis thaliana |
| title_fullStr | Protein Phosphorylation Changes During Systemic Acquired Resistance in Arabidopsis thaliana |
| title_full_unstemmed | Protein Phosphorylation Changes During Systemic Acquired Resistance in Arabidopsis thaliana |
| title_short | Protein Phosphorylation Changes During Systemic Acquired Resistance in Arabidopsis thaliana |
| title_sort | protein phosphorylation changes during systemic acquired resistance in arabidopsis thaliana |
| topic | phosphoproteomics Arabidopsis systemic acquired resistance data-independent acquisition calcium-dependent protein kinase (CDPK) MAPK |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.748287/full |
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