Variation in Ovine <i>DGAT1</i> and Its Association with Carcass Muscle Traits in Southdown Sheep
Diacylglycerol O-acyltransferase 1 (DGAT1) is a microsomal enzyme that plays a key role in the synthesis of triglycerides. Its gene (<i>DGAT1</i>) is regarded as a candidate gene for variation in milk and meat traits in cattle. The objective of this study was to use a PCR single-strand c...
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MDPI AG
2022-09-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/13/9/1670 |
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| author | Rong Dai Huitong Zhou Qian Fang Ping Zhou Yang Yang Shuang Jiang Jonathan G. H. Hickford |
| author_facet | Rong Dai Huitong Zhou Qian Fang Ping Zhou Yang Yang Shuang Jiang Jonathan G. H. Hickford |
| author_sort | Rong Dai |
| collection | DOAJ |
| description | Diacylglycerol O-acyltransferase 1 (DGAT1) is a microsomal enzyme that plays a key role in the synthesis of triglycerides. Its gene (<i>DGAT1</i>) is regarded as a candidate gene for variation in milk and meat traits in cattle. The objective of this study was to use a PCR single-strand conformation polymorphism approach to explore sequence variation in two regions of ovine <i>DGAT1</i> and to assess its effect on meat traits in New Zealand Southdown sheep. Three variant nucleotide sequences were identified in each region, with two single nucleotide polymorphisms (SNPs) and one nucleotide deletion being detected in intron 1 and two SNPs being found in exon 17. The effect of the exon 17 variation was not investigated due to one variant being predominant and the other two variants occurring at low frequencies. In intron 1, one variant (<i>B</i><sub>1</sub>) was found to be associated with increase loin meat yield, suggesting that this may have value as a gene marker for improving meat traits. |
| first_indexed | 2024-03-09T23:55:45Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T23:55:45Z |
| publishDate | 2022-09-01 |
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| series | Genes |
| spelling | doaj.art-aee9b0e7380b4f20841bf73f63a44f4b2023-11-23T16:26:14ZengMDPI AGGenes2073-44252022-09-01139167010.3390/genes13091670Variation in Ovine <i>DGAT1</i> and Its Association with Carcass Muscle Traits in Southdown SheepRong Dai0Huitong Zhou1Qian Fang2Ping Zhou3Yang Yang4Shuang Jiang5Jonathan G. H. Hickford6Xingjiang Academy of Agricultural and Reclamation Science/State Key Laboratory of Sheep Genetic Improvement and Healthy Production, Shihezi 832000, ChinaGene-Marker Laboratory, Department of Agricultural Sciences, Lincoln University, Lincoln 7647, New ZealandGene-Marker Laboratory, Department of Agricultural Sciences, Lincoln University, Lincoln 7647, New ZealandXingjiang Academy of Agricultural and Reclamation Science/State Key Laboratory of Sheep Genetic Improvement and Healthy Production, Shihezi 832000, ChinaXingjiang Academy of Agricultural and Reclamation Science/State Key Laboratory of Sheep Genetic Improvement and Healthy Production, Shihezi 832000, ChinaGene-Marker Laboratory, Department of Agricultural Sciences, Lincoln University, Lincoln 7647, New ZealandGene-Marker Laboratory, Department of Agricultural Sciences, Lincoln University, Lincoln 7647, New ZealandDiacylglycerol O-acyltransferase 1 (DGAT1) is a microsomal enzyme that plays a key role in the synthesis of triglycerides. Its gene (<i>DGAT1</i>) is regarded as a candidate gene for variation in milk and meat traits in cattle. The objective of this study was to use a PCR single-strand conformation polymorphism approach to explore sequence variation in two regions of ovine <i>DGAT1</i> and to assess its effect on meat traits in New Zealand Southdown sheep. Three variant nucleotide sequences were identified in each region, with two single nucleotide polymorphisms (SNPs) and one nucleotide deletion being detected in intron 1 and two SNPs being found in exon 17. The effect of the exon 17 variation was not investigated due to one variant being predominant and the other two variants occurring at low frequencies. In intron 1, one variant (<i>B</i><sub>1</sub>) was found to be associated with increase loin meat yield, suggesting that this may have value as a gene marker for improving meat traits.https://www.mdpi.com/2073-4425/13/9/1670diacylglycerol O-acyltransferase 1 genepolymorphismPCR-SSCPlambloin meat yield |
| spellingShingle | Rong Dai Huitong Zhou Qian Fang Ping Zhou Yang Yang Shuang Jiang Jonathan G. H. Hickford Variation in Ovine <i>DGAT1</i> and Its Association with Carcass Muscle Traits in Southdown Sheep Genes diacylglycerol O-acyltransferase 1 gene polymorphism PCR-SSCP lamb loin meat yield |
| title | Variation in Ovine <i>DGAT1</i> and Its Association with Carcass Muscle Traits in Southdown Sheep |
| title_full | Variation in Ovine <i>DGAT1</i> and Its Association with Carcass Muscle Traits in Southdown Sheep |
| title_fullStr | Variation in Ovine <i>DGAT1</i> and Its Association with Carcass Muscle Traits in Southdown Sheep |
| title_full_unstemmed | Variation in Ovine <i>DGAT1</i> and Its Association with Carcass Muscle Traits in Southdown Sheep |
| title_short | Variation in Ovine <i>DGAT1</i> and Its Association with Carcass Muscle Traits in Southdown Sheep |
| title_sort | variation in ovine i dgat1 i and its association with carcass muscle traits in southdown sheep |
| topic | diacylglycerol O-acyltransferase 1 gene polymorphism PCR-SSCP lamb loin meat yield |
| url | https://www.mdpi.com/2073-4425/13/9/1670 |
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