Functions of COP1/SPA E3 Ubiquitin Ligase Mediated by MpCRY in the Liverwort <i>Marchantia polymorpha</i> under Blue Light
COP1/SPA1 complex in <i>Arabidopsis</i> inhibits photomorphogenesis through the ubiquitination of multiple photo-responsive transcription factors in darkness, but such inhibiting function of COP1/SPA1 complex would be suppressed by cryptochromes in blue light. Extensive studies have been...
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MDPI AG
2021-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/1/158 |
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| author | Li Zhang Tianhong Li Shengzhong Su Hao Peng Sudi Li Ke Li Luyao Ji Yaoyun Xing Junchuan Zhang Xinglin Du Mingdi Bian Yuying Liao Zhenming Yang Zecheng Zuo |
| author_facet | Li Zhang Tianhong Li Shengzhong Su Hao Peng Sudi Li Ke Li Luyao Ji Yaoyun Xing Junchuan Zhang Xinglin Du Mingdi Bian Yuying Liao Zhenming Yang Zecheng Zuo |
| author_sort | Li Zhang |
| collection | DOAJ |
| description | COP1/SPA1 complex in <i>Arabidopsis</i> inhibits photomorphogenesis through the ubiquitination of multiple photo-responsive transcription factors in darkness, but such inhibiting function of COP1/SPA1 complex would be suppressed by cryptochromes in blue light. Extensive studies have been conducted on these mechanisms in <i>Arabidopsis</i> whereas little attention has been focused on whether another branch of land plants bryophyte utilizes this blue-light regulatory pathway. To study this problem, we conducted a study in the liverwort <i>Marchantia polymorpha</i> and obtained a MpSPA knock-out mutant, in which Mp<i>spa</i> exhibits the phenotype of an increased percentage of individuals with asymmetrical thallus growth, similar to MpCRY knock-out mutant. We also verified interactions of MpSPA with MpCRY (in a blue light-independent way) and with MpCOP1. Concomitantly, both MpSPA and MpCOP1 could interact with MpHY5, and MpSPA can promote MpCOP1 to ubiquitinate MpHY5 but MpCRY does not regulate the ubiquitination of MpHY5 by MpCOP1/MpSPA complex. These data suggest that COP1/SPA ubiquitinating HY5 is conserved in <i>Marchantia polymorpha</i>, but dissimilar to CRY in <i>Arabidopsis</i>, MpCRY is not an inhibitor of this process under blue light. |
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| language | English |
| last_indexed | 2024-03-10T03:39:52Z |
| publishDate | 2021-12-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-aef120767798400ea9ee759eb7d6629b2023-11-23T11:35:42ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-12-0123115810.3390/ijms23010158Functions of COP1/SPA E3 Ubiquitin Ligase Mediated by MpCRY in the Liverwort <i>Marchantia polymorpha</i> under Blue LightLi Zhang0Tianhong Li1Shengzhong Su2Hao Peng3Sudi Li4Ke Li5Luyao Ji6Yaoyun Xing7Junchuan Zhang8Xinglin Du9Mingdi Bian10Yuying Liao11Zhenming Yang12Zecheng Zuo13Jilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin University, Changchun 130062, ChinaJilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin University, Changchun 130062, ChinaJilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin University, Changchun 130062, ChinaGuangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning 530001, ChinaJilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin University, Changchun 130062, ChinaBasic Forestry and Proteomics Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaBasic Forestry and Proteomics Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaBasic Forestry and Proteomics Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaJilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin University, Changchun 130062, ChinaJilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin University, Changchun 130062, ChinaJilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin University, Changchun 130062, ChinaGuangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning 530001, ChinaJilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin University, Changchun 130062, ChinaJilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin University, Changchun 130062, ChinaCOP1/SPA1 complex in <i>Arabidopsis</i> inhibits photomorphogenesis through the ubiquitination of multiple photo-responsive transcription factors in darkness, but such inhibiting function of COP1/SPA1 complex would be suppressed by cryptochromes in blue light. Extensive studies have been conducted on these mechanisms in <i>Arabidopsis</i> whereas little attention has been focused on whether another branch of land plants bryophyte utilizes this blue-light regulatory pathway. To study this problem, we conducted a study in the liverwort <i>Marchantia polymorpha</i> and obtained a MpSPA knock-out mutant, in which Mp<i>spa</i> exhibits the phenotype of an increased percentage of individuals with asymmetrical thallus growth, similar to MpCRY knock-out mutant. We also verified interactions of MpSPA with MpCRY (in a blue light-independent way) and with MpCOP1. Concomitantly, both MpSPA and MpCOP1 could interact with MpHY5, and MpSPA can promote MpCOP1 to ubiquitinate MpHY5 but MpCRY does not regulate the ubiquitination of MpHY5 by MpCOP1/MpSPA complex. These data suggest that COP1/SPA ubiquitinating HY5 is conserved in <i>Marchantia polymorpha</i>, but dissimilar to CRY in <i>Arabidopsis</i>, MpCRY is not an inhibitor of this process under blue light.https://www.mdpi.com/1422-0067/23/1/158blue light<i>Marchantia polymorpha</i>cryptochromesCOP1/SPA complexHY5ubiquitination |
| spellingShingle | Li Zhang Tianhong Li Shengzhong Su Hao Peng Sudi Li Ke Li Luyao Ji Yaoyun Xing Junchuan Zhang Xinglin Du Mingdi Bian Yuying Liao Zhenming Yang Zecheng Zuo Functions of COP1/SPA E3 Ubiquitin Ligase Mediated by MpCRY in the Liverwort <i>Marchantia polymorpha</i> under Blue Light International Journal of Molecular Sciences blue light <i>Marchantia polymorpha</i> cryptochromes COP1/SPA complex HY5 ubiquitination |
| title | Functions of COP1/SPA E3 Ubiquitin Ligase Mediated by MpCRY in the Liverwort <i>Marchantia polymorpha</i> under Blue Light |
| title_full | Functions of COP1/SPA E3 Ubiquitin Ligase Mediated by MpCRY in the Liverwort <i>Marchantia polymorpha</i> under Blue Light |
| title_fullStr | Functions of COP1/SPA E3 Ubiquitin Ligase Mediated by MpCRY in the Liverwort <i>Marchantia polymorpha</i> under Blue Light |
| title_full_unstemmed | Functions of COP1/SPA E3 Ubiquitin Ligase Mediated by MpCRY in the Liverwort <i>Marchantia polymorpha</i> under Blue Light |
| title_short | Functions of COP1/SPA E3 Ubiquitin Ligase Mediated by MpCRY in the Liverwort <i>Marchantia polymorpha</i> under Blue Light |
| title_sort | functions of cop1 spa e3 ubiquitin ligase mediated by mpcry in the liverwort i marchantia polymorpha i under blue light |
| topic | blue light <i>Marchantia polymorpha</i> cryptochromes COP1/SPA complex HY5 ubiquitination |
| url | https://www.mdpi.com/1422-0067/23/1/158 |
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