Transdermal Immunization of Elastic Liposome-Laden Recombinant Chimeric Fusion Protein of <i>P. falciparum</i> (<i>Pf</i>MSP-Fu<sub>24</sub>) Mounts Protective Immune Response
Transdermal immunization exhibits poor immunogenic responses due to poor permeability of antigens through the skin. Elastic liposomes, the ultradeformable nanoscale lipid vesicles, overcome the permeability issues and prove a versatile nanocarrier for transcutaneous delivery of protein, peptide, and...
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MDPI AG
2021-02-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/11/2/406 |
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| author | Ramesh Chaudhari Nikunj Tandel Kiran Sahu Sushmita Negi Hilal Bashir Arzu Rupareliya Ravi PN Mishra Sarat K. Dalai Rajeev K. Tyagi |
| author_facet | Ramesh Chaudhari Nikunj Tandel Kiran Sahu Sushmita Negi Hilal Bashir Arzu Rupareliya Ravi PN Mishra Sarat K. Dalai Rajeev K. Tyagi |
| author_sort | Ramesh Chaudhari |
| collection | DOAJ |
| description | Transdermal immunization exhibits poor immunogenic responses due to poor permeability of antigens through the skin. Elastic liposomes, the ultradeformable nanoscale lipid vesicles, overcome the permeability issues and prove a versatile nanocarrier for transcutaneous delivery of protein, peptide, and nucleic acid antigens. Elastic liposome-mediated subcutaneous delivery of chimeric fusion protein (<i>Pf</i>MSP-Fu<sub>24</sub>) of <i>Plasmodium falciparum</i> exhibited improved immunogenic responses. Elastic liposomes-mediated immunization of <i>Pf</i>MSP-Fu<sub>24</sub> conferred immunity to the asexual blood-stage infection. Present study is an attempt to compare the protective immune response mounted by the <i>Pf</i>MSP-Fu<sub>24</sub> upon administered through transdermal and intramuscular routes. Humoral and cell-mediated immune (CMI) response elicited by topical and intramuscularly administered <i>Pf</i>MSP-Fu<sub>24</sub>-laden elastic liposomes (EL-<i>Pf</i>MSP-Fu<sub>24</sub>) were compared and normalized with the vehicle control. Sizeable immune responses were seen with the transcutaneously immunized EL-<i>Pf</i>MSP-Fu<sub>24</sub> and compared with those elicited with intramuscularly administered antigen. Our results show significant IgG isotype subclass (IgG1and IgG3) response of specific antibody levels as well as cell-mediated immunity (CMI) activating factor (IFN-γ), a crucial player in conferring resistance to blood-stage malaria in mice receiving EL-<i>Pf</i>MSP-Fu<sub>24</sub> through transdermal route as compared to the intramuscularly administered formulation. Heightened immune response obtained by the vaccination of EL-<i>Pf</i>MSP-Fu<sub>24</sub> was complemented by the quantification of the transcript (mRNA) levels cell-mediated (IFN-γ, IL-4), and regulatory immune response (IL-10) in the lymph nodes and spleen. Collectively, elastic liposomes prove their immune-adjuvant property as they evoke sizeable and perdurable immune response against <i>Pf</i>MSP-Fu<sub>24</sub> and justify its potential for the improved vaccine delivery to inducing both humoral and CM immune response. |
| first_indexed | 2024-03-09T05:31:35Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T05:31:35Z |
| publishDate | 2021-02-01 |
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| series | Nanomaterials |
| spelling | doaj.art-aef1567bb3f84226be363a66fb3127522023-12-03T12:32:01ZengMDPI AGNanomaterials2079-49912021-02-0111240610.3390/nano11020406Transdermal Immunization of Elastic Liposome-Laden Recombinant Chimeric Fusion Protein of <i>P. falciparum</i> (<i>Pf</i>MSP-Fu<sub>24</sub>) Mounts Protective Immune ResponseRamesh Chaudhari0Nikunj Tandel1Kiran Sahu2Sushmita Negi3Hilal Bashir4Arzu Rupareliya5Ravi PN Mishra6Sarat K. Dalai7Rajeev K. Tyagi8Institute of Science, Nirma University, Ahmedabad 382481, Gujarat, IndiaInstitute of Science, Nirma University, Ahmedabad 382481, Gujarat, IndiaDivision of Cell Biology and Immunology, Biomedical Parasitology and Nano-Immunology Lab., CSIR-Institute of Microbial Technology (IMTECH), Sec-39A, Chandigarh 160036, IndiaDivision of Cell Biology and Immunology, Biomedical Parasitology and Nano-Immunology Lab., CSIR-Institute of Microbial Technology (IMTECH), Sec-39A, Chandigarh 160036, IndiaDivision of Cell Biology and Immunology, CSIR-Institute of Microbial Technology (IMTECH), Sec-39A, Chandigarh 160036, IndiaInstitute of Science, Nirma University, Ahmedabad 382481, Gujarat, IndiaBERPDC Department, CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh 160036, IndiaInstitute of Science, Nirma University, Ahmedabad 382481, Gujarat, IndiaDivision of Cell Biology and Immunology, Biomedical Parasitology and Nano-Immunology Lab., CSIR-Institute of Microbial Technology (IMTECH), Sec-39A, Chandigarh 160036, IndiaTransdermal immunization exhibits poor immunogenic responses due to poor permeability of antigens through the skin. Elastic liposomes, the ultradeformable nanoscale lipid vesicles, overcome the permeability issues and prove a versatile nanocarrier for transcutaneous delivery of protein, peptide, and nucleic acid antigens. Elastic liposome-mediated subcutaneous delivery of chimeric fusion protein (<i>Pf</i>MSP-Fu<sub>24</sub>) of <i>Plasmodium falciparum</i> exhibited improved immunogenic responses. Elastic liposomes-mediated immunization of <i>Pf</i>MSP-Fu<sub>24</sub> conferred immunity to the asexual blood-stage infection. Present study is an attempt to compare the protective immune response mounted by the <i>Pf</i>MSP-Fu<sub>24</sub> upon administered through transdermal and intramuscular routes. Humoral and cell-mediated immune (CMI) response elicited by topical and intramuscularly administered <i>Pf</i>MSP-Fu<sub>24</sub>-laden elastic liposomes (EL-<i>Pf</i>MSP-Fu<sub>24</sub>) were compared and normalized with the vehicle control. Sizeable immune responses were seen with the transcutaneously immunized EL-<i>Pf</i>MSP-Fu<sub>24</sub> and compared with those elicited with intramuscularly administered antigen. Our results show significant IgG isotype subclass (IgG1and IgG3) response of specific antibody levels as well as cell-mediated immunity (CMI) activating factor (IFN-γ), a crucial player in conferring resistance to blood-stage malaria in mice receiving EL-<i>Pf</i>MSP-Fu<sub>24</sub> through transdermal route as compared to the intramuscularly administered formulation. Heightened immune response obtained by the vaccination of EL-<i>Pf</i>MSP-Fu<sub>24</sub> was complemented by the quantification of the transcript (mRNA) levels cell-mediated (IFN-γ, IL-4), and regulatory immune response (IL-10) in the lymph nodes and spleen. Collectively, elastic liposomes prove their immune-adjuvant property as they evoke sizeable and perdurable immune response against <i>Pf</i>MSP-Fu<sub>24</sub> and justify its potential for the improved vaccine delivery to inducing both humoral and CM immune response.https://www.mdpi.com/2079-4991/11/2/406malariaelastic liposomes<i>Pf</i>MSP-Fu<sub>24</sub>humoral and cellular immunityvaccine |
| spellingShingle | Ramesh Chaudhari Nikunj Tandel Kiran Sahu Sushmita Negi Hilal Bashir Arzu Rupareliya Ravi PN Mishra Sarat K. Dalai Rajeev K. Tyagi Transdermal Immunization of Elastic Liposome-Laden Recombinant Chimeric Fusion Protein of <i>P. falciparum</i> (<i>Pf</i>MSP-Fu<sub>24</sub>) Mounts Protective Immune Response Nanomaterials malaria elastic liposomes <i>Pf</i>MSP-Fu<sub>24</sub> humoral and cellular immunity vaccine |
| title | Transdermal Immunization of Elastic Liposome-Laden Recombinant Chimeric Fusion Protein of <i>P. falciparum</i> (<i>Pf</i>MSP-Fu<sub>24</sub>) Mounts Protective Immune Response |
| title_full | Transdermal Immunization of Elastic Liposome-Laden Recombinant Chimeric Fusion Protein of <i>P. falciparum</i> (<i>Pf</i>MSP-Fu<sub>24</sub>) Mounts Protective Immune Response |
| title_fullStr | Transdermal Immunization of Elastic Liposome-Laden Recombinant Chimeric Fusion Protein of <i>P. falciparum</i> (<i>Pf</i>MSP-Fu<sub>24</sub>) Mounts Protective Immune Response |
| title_full_unstemmed | Transdermal Immunization of Elastic Liposome-Laden Recombinant Chimeric Fusion Protein of <i>P. falciparum</i> (<i>Pf</i>MSP-Fu<sub>24</sub>) Mounts Protective Immune Response |
| title_short | Transdermal Immunization of Elastic Liposome-Laden Recombinant Chimeric Fusion Protein of <i>P. falciparum</i> (<i>Pf</i>MSP-Fu<sub>24</sub>) Mounts Protective Immune Response |
| title_sort | transdermal immunization of elastic liposome laden recombinant chimeric fusion protein of i p falciparum i i pf i msp fu sub 24 sub mounts protective immune response |
| topic | malaria elastic liposomes <i>Pf</i>MSP-Fu<sub>24</sub> humoral and cellular immunity vaccine |
| url | https://www.mdpi.com/2079-4991/11/2/406 |
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