In-vivo egfp expression in the honeybee Apis mellifera induced by electroporation and viral expression vector
In this study we describe egfp expression induced by two techniques: in vivo electroporation and viral transduction in several cell types of the adult honeybee brain. Non-neuronal and neuronal cell types were identified and the expression persisted at least during three days. Kenyon cells, optic lob...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9162312/?tool=EBI |
| _version_ | 1811336221444538368 |
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| author | Gérard Leboulle Nora Gehne Anja Froese Randolf Menzel |
| author_facet | Gérard Leboulle Nora Gehne Anja Froese Randolf Menzel |
| author_sort | Gérard Leboulle |
| collection | DOAJ |
| description | In this study we describe egfp expression induced by two techniques: in vivo electroporation and viral transduction in several cell types of the adult honeybee brain. Non-neuronal and neuronal cell types were identified and the expression persisted at least during three days. Kenyon cells, optic lobe neurons and protocerebral lobe neurons were electroporated. Astrocyte-like glia cells, fibrous lamellar glia cells and cortex glia cells were identified. Viral transduction targeted one specific type of glia cells that could not be identified. EGFP positive cells types were rather variable after electroporation, and viral transduction resulted in more homogenous groups of positive cells. We propose that these techniques remain a good alternative to transgenic animals because they potentially target only somatic cells. |
| first_indexed | 2024-04-13T17:36:21Z |
| format | Article |
| id | doaj.art-aef29e51b60b4295a98a9becb1754a93 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T17:36:21Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-aef29e51b60b4295a98a9becb1754a932022-12-22T02:37:21ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01176In-vivo egfp expression in the honeybee Apis mellifera induced by electroporation and viral expression vectorGérard LeboulleNora GehneAnja FroeseRandolf MenzelIn this study we describe egfp expression induced by two techniques: in vivo electroporation and viral transduction in several cell types of the adult honeybee brain. Non-neuronal and neuronal cell types were identified and the expression persisted at least during three days. Kenyon cells, optic lobe neurons and protocerebral lobe neurons were electroporated. Astrocyte-like glia cells, fibrous lamellar glia cells and cortex glia cells were identified. Viral transduction targeted one specific type of glia cells that could not be identified. EGFP positive cells types were rather variable after electroporation, and viral transduction resulted in more homogenous groups of positive cells. We propose that these techniques remain a good alternative to transgenic animals because they potentially target only somatic cells.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9162312/?tool=EBI |
| spellingShingle | Gérard Leboulle Nora Gehne Anja Froese Randolf Menzel In-vivo egfp expression in the honeybee Apis mellifera induced by electroporation and viral expression vector PLoS ONE |
| title | In-vivo egfp expression in the honeybee Apis mellifera induced by electroporation and viral expression vector |
| title_full | In-vivo egfp expression in the honeybee Apis mellifera induced by electroporation and viral expression vector |
| title_fullStr | In-vivo egfp expression in the honeybee Apis mellifera induced by electroporation and viral expression vector |
| title_full_unstemmed | In-vivo egfp expression in the honeybee Apis mellifera induced by electroporation and viral expression vector |
| title_short | In-vivo egfp expression in the honeybee Apis mellifera induced by electroporation and viral expression vector |
| title_sort | in vivo egfp expression in the honeybee apis mellifera induced by electroporation and viral expression vector |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9162312/?tool=EBI |
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