Genetic changes in grapevine genomes after stress induced by in vitro cultivation, thermotherapy and virus infection, as revealed by AFLP
The Amplification Fragment Length Polymorphism (AFLP) technique was employed to study genetic variations which can be induced in vines by the stress occurring during different aspects of viticulture (in vitro cultivation, in vitro thermotherapy and virus infection). Analysis of AFLP banding patterns...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2009-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400026 |
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| author | Miroslav Baránek Jana Raddová Bretislav Krizan Miroslav Pidra |
| author_facet | Miroslav Baránek Jana Raddová Bretislav Krizan Miroslav Pidra |
| author_sort | Miroslav Baránek |
| collection | DOAJ |
| description | The Amplification Fragment Length Polymorphism (AFLP) technique was employed to study genetic variations which can be induced in vines by the stress occurring during different aspects of viticulture (in vitro cultivation, in vitro thermotherapy and virus infection). Analysis of AFLP banding patterns, generated by using 15 primer combinations, pointed to negligible genetic variation among plants exposed to individual stress. The average of similarity coefficients between differently stressed plants of the cultivars Müller Thurgau and Riesling were 0.984 and 0.991, respectively, as revealed by AFLP analysis. The low incidence of observed polymorphism demonstrates the high level of genome uniformity in plants reproduced by in vitro micropropagation via nodes, those subjected to in vitro thermotherapy and virus-infected plants. |
| first_indexed | 2024-12-21T14:18:20Z |
| format | Article |
| id | doaj.art-aef32a2c88cd493cadd6a84c29437731 |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-21T14:18:20Z |
| publishDate | 2009-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-aef32a2c88cd493cadd6a84c294377312022-12-21T19:00:51ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852009-01-01324834839Genetic changes in grapevine genomes after stress induced by in vitro cultivation, thermotherapy and virus infection, as revealed by AFLPMiroslav BaránekJana RaddováBretislav KrizanMiroslav PidraThe Amplification Fragment Length Polymorphism (AFLP) technique was employed to study genetic variations which can be induced in vines by the stress occurring during different aspects of viticulture (in vitro cultivation, in vitro thermotherapy and virus infection). Analysis of AFLP banding patterns, generated by using 15 primer combinations, pointed to negligible genetic variation among plants exposed to individual stress. The average of similarity coefficients between differently stressed plants of the cultivars Müller Thurgau and Riesling were 0.984 and 0.991, respectively, as revealed by AFLP analysis. The low incidence of observed polymorphism demonstrates the high level of genome uniformity in plants reproduced by in vitro micropropagation via nodes, those subjected to in vitro thermotherapy and virus-infected plants.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400026grapevinestresssomaclonal variationvirus infectionAFLP |
| spellingShingle | Miroslav Baránek Jana Raddová Bretislav Krizan Miroslav Pidra Genetic changes in grapevine genomes after stress induced by in vitro cultivation, thermotherapy and virus infection, as revealed by AFLP Genetics and Molecular Biology grapevine stress somaclonal variation virus infection AFLP |
| title | Genetic changes in grapevine genomes after stress induced by in vitro cultivation, thermotherapy and virus infection, as revealed by AFLP |
| title_full | Genetic changes in grapevine genomes after stress induced by in vitro cultivation, thermotherapy and virus infection, as revealed by AFLP |
| title_fullStr | Genetic changes in grapevine genomes after stress induced by in vitro cultivation, thermotherapy and virus infection, as revealed by AFLP |
| title_full_unstemmed | Genetic changes in grapevine genomes after stress induced by in vitro cultivation, thermotherapy and virus infection, as revealed by AFLP |
| title_short | Genetic changes in grapevine genomes after stress induced by in vitro cultivation, thermotherapy and virus infection, as revealed by AFLP |
| title_sort | genetic changes in grapevine genomes after stress induced by in vitro cultivation thermotherapy and virus infection as revealed by aflp |
| topic | grapevine stress somaclonal variation virus infection AFLP |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400026 |
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