Effects of different types of gelling agents on in vitro organogenesis and some physicochemical properties of date palm buds, Showathy cv.
Some obstacles are associated with in vitro propagation of date palm, such as explant tissue browning, slow callus growth and development, low organogenesis and multiplication efficiency, and frequent tissue vitrification. This investigation studied the effect of five types of gelling agents (Danish...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Sciendo
2021-05-01
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| Series: | Folia Oecologica |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/foecol-2021-0012 |
| _version_ | 1818908949416509440 |
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| author | Al-Mayahi Ahmed Madi Waheed Ali Abdulminam Hussian |
| author_facet | Al-Mayahi Ahmed Madi Waheed Ali Abdulminam Hussian |
| author_sort | Al-Mayahi Ahmed Madi Waheed |
| collection | DOAJ |
| description | Some obstacles are associated with in vitro propagation of date palm, such as explant tissue browning, slow callus growth and development, low organogenesis and multiplication efficiency, and frequent tissue vitrification. This investigation studied the effect of five types of gelling agents (Danish Agar, Cero Agar Type 8952, Chile Agar, Gerlite Food Grade, and Agar-Agar.) on in vitro regeneration and bud multiplication of Phoenix dactylifera L. cv. Showathy. The results showed that the highest percentages of callus producing buds and average bud formation (77.78%, 11.5 buds, and 72.23%, 10.9 buds) were obtained in response to 7 g l–1 Danish Agar and Cero Agar Type 8952, respectively. A decrease in browning percentage was observed in tissues cultured in the medium gelled with Danish Agar. Observations showed that Danish Agar and Cero Agar Type 8952 eliminated also shoot vitrification. Compared with other treatments, the total amount of phenolic compounds was significantly reduced to 0.79 and 0.82 mg GAE/g in buds cultured in the media gelled with Danish Agar and Cero Agar Type 8952, respectively. The macronutrient phosphor, calcium, sodium, and micronutrient boron and copper significantly increased in the in vitro shoots regenerated on the media gelled with Danish Agar and Cero Agar Type 8952. |
| first_indexed | 2024-12-19T22:19:08Z |
| format | Article |
| id | doaj.art-aef69d993ba842c3b30fac965df38343 |
| institution | Directory Open Access Journal |
| issn | 1338-7014 |
| language | English |
| last_indexed | 2024-12-19T22:19:08Z |
| publishDate | 2021-05-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Folia Oecologica |
| spelling | doaj.art-aef69d993ba842c3b30fac965df383432022-12-21T20:03:41ZengSciendoFolia Oecologica1338-70142021-05-0148111011710.2478/foecol-2021-0012Effects of different types of gelling agents on in vitro organogenesis and some physicochemical properties of date palm buds, Showathy cv.Al-Mayahi Ahmed Madi Waheed0Ali Abdulminam Hussian1Date Palm Research Centre, University of Basrah, Basrah, IraqScience College, Basrah University, Basrah, IraqSome obstacles are associated with in vitro propagation of date palm, such as explant tissue browning, slow callus growth and development, low organogenesis and multiplication efficiency, and frequent tissue vitrification. This investigation studied the effect of five types of gelling agents (Danish Agar, Cero Agar Type 8952, Chile Agar, Gerlite Food Grade, and Agar-Agar.) on in vitro regeneration and bud multiplication of Phoenix dactylifera L. cv. Showathy. The results showed that the highest percentages of callus producing buds and average bud formation (77.78%, 11.5 buds, and 72.23%, 10.9 buds) were obtained in response to 7 g l–1 Danish Agar and Cero Agar Type 8952, respectively. A decrease in browning percentage was observed in tissues cultured in the medium gelled with Danish Agar. Observations showed that Danish Agar and Cero Agar Type 8952 eliminated also shoot vitrification. Compared with other treatments, the total amount of phenolic compounds was significantly reduced to 0.79 and 0.82 mg GAE/g in buds cultured in the media gelled with Danish Agar and Cero Agar Type 8952, respectively. The macronutrient phosphor, calcium, sodium, and micronutrient boron and copper significantly increased in the in vitro shoots regenerated on the media gelled with Danish Agar and Cero Agar Type 8952.https://doi.org/10.2478/foecol-2021-0012browningmultiplicationnutrientsphenol compoundsvitrification |
| spellingShingle | Al-Mayahi Ahmed Madi Waheed Ali Abdulminam Hussian Effects of different types of gelling agents on in vitro organogenesis and some physicochemical properties of date palm buds, Showathy cv. Folia Oecologica browning multiplication nutrients phenol compounds vitrification |
| title | Effects of different types of gelling agents on in vitro organogenesis and some physicochemical properties of date palm buds, Showathy cv. |
| title_full | Effects of different types of gelling agents on in vitro organogenesis and some physicochemical properties of date palm buds, Showathy cv. |
| title_fullStr | Effects of different types of gelling agents on in vitro organogenesis and some physicochemical properties of date palm buds, Showathy cv. |
| title_full_unstemmed | Effects of different types of gelling agents on in vitro organogenesis and some physicochemical properties of date palm buds, Showathy cv. |
| title_short | Effects of different types of gelling agents on in vitro organogenesis and some physicochemical properties of date palm buds, Showathy cv. |
| title_sort | effects of different types of gelling agents on in vitro organogenesis and some physicochemical properties of date palm buds showathy cv |
| topic | browning multiplication nutrients phenol compounds vitrification |
| url | https://doi.org/10.2478/foecol-2021-0012 |
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