Homologous ELISA for detection of prednisolone in human serum
An enzyme-linked immunosorbent assay to measure prednisolone have been developed. An antibody was raised in rabbits using prednisolone-21-hemisuccinate (PSL-21-HS) conjugated to bovine serum albumin. Similarly, PSL-21-HS was conjugated to horseradish peroxidase to prepare enzyme conjugate. The devel...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-01-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2017.1376184 |
| _version_ | 1818394674153390080 |
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| author | Dinesh Kumar Harpal Singh T. G. Shrivastav |
| author_facet | Dinesh Kumar Harpal Singh T. G. Shrivastav |
| author_sort | Dinesh Kumar |
| collection | DOAJ |
| description | An enzyme-linked immunosorbent assay to measure prednisolone have been developed. An antibody was raised in rabbits using prednisolone-21-hemisuccinate (PSL-21-HS) conjugated to bovine serum albumin. Similarly, PSL-21-HS was conjugated to horseradish peroxidase to prepare enzyme conjugate. The developed assay has been validated for sensitivity and effective displacement at 50% of the assay were 0.078 and 2.64 ng/mL, respectively. This assay showed cross-reaction with only 4 steroids – i.e. progesterone 1.76%, 17α-OH progesterone 5.89%, cortisol 7.69% and prednisone 1.13%, out of 55 analogous steroids. The percent recovery of prednisolone from the exogenously spiked human serum pools was in the range of 94.84–100.17%. The intra-assay and inter-assay coefficients of variation ranged from 5.79% to 8.00% and from 3.23% to 8.63%, respectively. The serum prednisolone values obtained by this method correlated well with the commercially available kit and found to be 0.93. |
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| format | Article |
| id | doaj.art-aef8e9e898674a6bbdf24a1c24e89de9 |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-12-14T06:04:57Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
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| series | Food and Agricultural Immunology |
| spelling | doaj.art-aef8e9e898674a6bbdf24a1c24e89de92022-12-21T23:14:20ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432018-01-0129136938510.1080/09540105.2017.13761841376184Homologous ELISA for detection of prednisolone in human serumDinesh Kumar0Harpal Singh1T. G. Shrivastav2Immuno, Isotope and Nano-technology Laboratory, Department of Reproductive Biomedicine, The National Institute of Health and Family Welfare (NIHFW)Indian Institute of Technology Delhi (IIT-D)Immuno, Isotope and Nano-technology Laboratory, Department of Reproductive Biomedicine, The National Institute of Health and Family Welfare (NIHFW)An enzyme-linked immunosorbent assay to measure prednisolone have been developed. An antibody was raised in rabbits using prednisolone-21-hemisuccinate (PSL-21-HS) conjugated to bovine serum albumin. Similarly, PSL-21-HS was conjugated to horseradish peroxidase to prepare enzyme conjugate. The developed assay has been validated for sensitivity and effective displacement at 50% of the assay were 0.078 and 2.64 ng/mL, respectively. This assay showed cross-reaction with only 4 steroids – i.e. progesterone 1.76%, 17α-OH progesterone 5.89%, cortisol 7.69% and prednisone 1.13%, out of 55 analogous steroids. The percent recovery of prednisolone from the exogenously spiked human serum pools was in the range of 94.84–100.17%. The intra-assay and inter-assay coefficients of variation ranged from 5.79% to 8.00% and from 3.23% to 8.63%, respectively. The serum prednisolone values obtained by this method correlated well with the commercially available kit and found to be 0.93.http://dx.doi.org/10.1080/09540105.2017.1376184prednisoloneimmunogenantibodyhorseradish peroxidaseelisa |
| spellingShingle | Dinesh Kumar Harpal Singh T. G. Shrivastav Homologous ELISA for detection of prednisolone in human serum Food and Agricultural Immunology prednisolone immunogen antibody horseradish peroxidase elisa |
| title | Homologous ELISA for detection of prednisolone in human serum |
| title_full | Homologous ELISA for detection of prednisolone in human serum |
| title_fullStr | Homologous ELISA for detection of prednisolone in human serum |
| title_full_unstemmed | Homologous ELISA for detection of prednisolone in human serum |
| title_short | Homologous ELISA for detection of prednisolone in human serum |
| title_sort | homologous elisa for detection of prednisolone in human serum |
| topic | prednisolone immunogen antibody horseradish peroxidase elisa |
| url | http://dx.doi.org/10.1080/09540105.2017.1376184 |
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