Cytological Analysis of Crossover Frequency and Distribution in Male Meiosis of Cardueline Finches (Fringillidae, Aves)
Meiotic recombination is an important source of genetic diversity. Using immunolocalization of several meiotic proteins at the spreads of male pachytene cells, we estimated the number of recombination nodules per cell and their distribution along the macrochromosome 1 of the <i>Common linnet&l...
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MDPI AG
2023-11-01
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| author | Ekaterina Grishko Lyubov Malinovskaya Anastasia Slobodchikova Artemiy Kotelnikov Anna Torgasheva Pavel Borodin |
| author_facet | Ekaterina Grishko Lyubov Malinovskaya Anastasia Slobodchikova Artemiy Kotelnikov Anna Torgasheva Pavel Borodin |
| author_sort | Ekaterina Grishko |
| collection | DOAJ |
| description | Meiotic recombination is an important source of genetic diversity. Using immunolocalization of several meiotic proteins at the spreads of male pachytene cells, we estimated the number of recombination nodules per cell and their distribution along the macrochromosome 1 of the <i>Common linnet</i>, <i>Eurasian bullfinch</i>, <i>Eurasian siskin</i>, and <i>European goldfinch</i>. The macrochromosomes of the two former species have metapolycentromeres, composed of several centromeric domains. We detected significant interspecies differences in the mean numbers of recombination nodules per genome: 52.9 ± 2.8 in the <i>linnet</i>, 49.5 ± 3.5 in the <i>bullfinch</i>, 61.5 ± 6.3 in the <i>siskin</i> and 52.2 ± 2.7 in the <i>goldfinch</i>. Recombination patterns on macrochromosome 1 were similar across species, with more nodules localized near chromosome ends and fewer around centromeres. The distance from the proximal nodule to the centromere depended on the nodule count per chromosome arm, with more events leading to a closer location. However, species with different centromere types showed no difference in this regard. We propose that the deficiency of recombination sites near centromeres could be due to the sequential occurrence of crossovers starting from the chromosome ends and may not be attributed to any suppressive effect of the centromere itself. |
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| spelling | doaj.art-aeffb4c1508b4022bfce0c5e9484f4642023-12-08T15:10:29ZengMDPI AGAnimals2076-26152023-11-011323362410.3390/ani13233624Cytological Analysis of Crossover Frequency and Distribution in Male Meiosis of Cardueline Finches (Fringillidae, Aves)Ekaterina Grishko0Lyubov Malinovskaya1Anastasia Slobodchikova2Artemiy Kotelnikov3Anna Torgasheva4Pavel Borodin5Department of Molecular Genetics, Cell Biology and Bioinformatics, Institute of Cytology and Genetics, 630090 Novosibirsk, RussiaDepartment of Molecular Genetics, Cell Biology and Bioinformatics, Institute of Cytology and Genetics, 630090 Novosibirsk, RussiaDepartment of Molecular Genetics, Cell Biology and Bioinformatics, Institute of Cytology and Genetics, 630090 Novosibirsk, RussiaDepartment of Molecular Genetics, Cell Biology and Bioinformatics, Institute of Cytology and Genetics, 630090 Novosibirsk, RussiaDepartment of Molecular Genetics, Cell Biology and Bioinformatics, Institute of Cytology and Genetics, 630090 Novosibirsk, RussiaDepartment of Molecular Genetics, Cell Biology and Bioinformatics, Institute of Cytology and Genetics, 630090 Novosibirsk, RussiaMeiotic recombination is an important source of genetic diversity. Using immunolocalization of several meiotic proteins at the spreads of male pachytene cells, we estimated the number of recombination nodules per cell and their distribution along the macrochromosome 1 of the <i>Common linnet</i>, <i>Eurasian bullfinch</i>, <i>Eurasian siskin</i>, and <i>European goldfinch</i>. The macrochromosomes of the two former species have metapolycentromeres, composed of several centromeric domains. We detected significant interspecies differences in the mean numbers of recombination nodules per genome: 52.9 ± 2.8 in the <i>linnet</i>, 49.5 ± 3.5 in the <i>bullfinch</i>, 61.5 ± 6.3 in the <i>siskin</i> and 52.2 ± 2.7 in the <i>goldfinch</i>. Recombination patterns on macrochromosome 1 were similar across species, with more nodules localized near chromosome ends and fewer around centromeres. The distance from the proximal nodule to the centromere depended on the nodule count per chromosome arm, with more events leading to a closer location. However, species with different centromere types showed no difference in this regard. We propose that the deficiency of recombination sites near centromeres could be due to the sequential occurrence of crossovers starting from the chromosome ends and may not be attributed to any suppressive effect of the centromere itself.https://www.mdpi.com/2076-2615/13/23/3624crossing overinterferencemetapolycentromeresmeta-polycentromerescentromere effect |
| spellingShingle | Ekaterina Grishko Lyubov Malinovskaya Anastasia Slobodchikova Artemiy Kotelnikov Anna Torgasheva Pavel Borodin Cytological Analysis of Crossover Frequency and Distribution in Male Meiosis of Cardueline Finches (Fringillidae, Aves) Animals crossing over interference metapolycentromeres meta-polycentromeres centromere effect |
| title | Cytological Analysis of Crossover Frequency and Distribution in Male Meiosis of Cardueline Finches (Fringillidae, Aves) |
| title_full | Cytological Analysis of Crossover Frequency and Distribution in Male Meiosis of Cardueline Finches (Fringillidae, Aves) |
| title_fullStr | Cytological Analysis of Crossover Frequency and Distribution in Male Meiosis of Cardueline Finches (Fringillidae, Aves) |
| title_full_unstemmed | Cytological Analysis of Crossover Frequency and Distribution in Male Meiosis of Cardueline Finches (Fringillidae, Aves) |
| title_short | Cytological Analysis of Crossover Frequency and Distribution in Male Meiosis of Cardueline Finches (Fringillidae, Aves) |
| title_sort | cytological analysis of crossover frequency and distribution in male meiosis of cardueline finches fringillidae aves |
| topic | crossing over interference metapolycentromeres meta-polycentromeres centromere effect |
| url | https://www.mdpi.com/2076-2615/13/23/3624 |
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