Plasmodium TatD-Like DNase Antibodies Blocked Parasite Development in the Mosquito Gut
The TatD-like DNase of Plasmodium species has previously been characterized as a conserved antigen that plays an important role in immune evasion. Here, we found that TatD-like DNase is expressed, apart from the erythrocytic stage, throughout the developmental stages of the parasite in the mosquito...
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| Format: | Article |
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Frontiers Media S.A.
2018-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.01023/full |
| _version_ | 1828396459701043200 |
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| author | Wei Wang Fei Liu Ning Jiang Huijun Lu Na Yang Ying Feng Xiaoyu Sang Yaming Cao Qijun Chen Qijun Chen |
| author_facet | Wei Wang Fei Liu Ning Jiang Huijun Lu Na Yang Ying Feng Xiaoyu Sang Yaming Cao Qijun Chen Qijun Chen |
| author_sort | Wei Wang |
| collection | DOAJ |
| description | The TatD-like DNase of Plasmodium species has previously been characterized as a conserved antigen that plays an important role in immune evasion. Here, we found that TatD-like DNase is expressed, apart from the erythrocytic stage, throughout the developmental stages of the parasite in the mosquito vector. Antibodies to the molecule significantly blocked parasites development and transition in the mosquito gut. Further, mice immunized with recombinant TatD-like DNase showed significant resistance to parasite challenge. The antigenicity of the TatD-like antigen in combination with various adjuvants, including Freund’s adjuvants, Montanide ISA 51 and 61, Alhydrogel (aluminum hydroxide), and levamisole was investigated. It was found that immunization of the recombinant TatD-like DNase in combination with Montanide ISA 51 induced strong humoral responses that showed significant protection against parasite challenge in a mouse model. The data further support that TatD-like DNase is a functionally important molecule in the whole development cycle of the malaria parasites and a candidate for malaria vaccine development. |
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| format | Article |
| id | doaj.art-af02e94518304b99814dbea48f9bcead |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-10T08:30:21Z |
| publishDate | 2018-05-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-af02e94518304b99814dbea48f9bcead2022-12-22T01:56:07ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-05-01910.3389/fmicb.2018.01023363203Plasmodium TatD-Like DNase Antibodies Blocked Parasite Development in the Mosquito GutWei Wang0Fei Liu1Ning Jiang2Huijun Lu3Na Yang4Ying Feng5Xiaoyu Sang6Yaming Cao7Qijun Chen8Qijun Chen9Key Laboratory of Zoonosis, Jilin University, Changchun, ChinaDepartment of Immunology, China Medical University, Shenyang, ChinaKey Laboratory of Zoonosis, Shenyang Agricultural University, Shenyang, ChinaKey Laboratory of Zoonosis, Jilin University, Changchun, ChinaKey Laboratory of Zoonosis, Shenyang Agricultural University, Shenyang, ChinaKey Laboratory of Zoonosis, Shenyang Agricultural University, Shenyang, ChinaKey Laboratory of Zoonosis, Shenyang Agricultural University, Shenyang, ChinaDepartment of Immunology, China Medical University, Shenyang, ChinaKey Laboratory of Zoonosis, Jilin University, Changchun, ChinaKey Laboratory of Zoonosis, Shenyang Agricultural University, Shenyang, ChinaThe TatD-like DNase of Plasmodium species has previously been characterized as a conserved antigen that plays an important role in immune evasion. Here, we found that TatD-like DNase is expressed, apart from the erythrocytic stage, throughout the developmental stages of the parasite in the mosquito vector. Antibodies to the molecule significantly blocked parasites development and transition in the mosquito gut. Further, mice immunized with recombinant TatD-like DNase showed significant resistance to parasite challenge. The antigenicity of the TatD-like antigen in combination with various adjuvants, including Freund’s adjuvants, Montanide ISA 51 and 61, Alhydrogel (aluminum hydroxide), and levamisole was investigated. It was found that immunization of the recombinant TatD-like DNase in combination with Montanide ISA 51 induced strong humoral responses that showed significant protection against parasite challenge in a mouse model. The data further support that TatD-like DNase is a functionally important molecule in the whole development cycle of the malaria parasites and a candidate for malaria vaccine development.https://www.frontiersin.org/article/10.3389/fmicb.2018.01023/fullplasmodiummalariaTAtD-like DNaseantibodytransmission blocking vaccine |
| spellingShingle | Wei Wang Fei Liu Ning Jiang Huijun Lu Na Yang Ying Feng Xiaoyu Sang Yaming Cao Qijun Chen Qijun Chen Plasmodium TatD-Like DNase Antibodies Blocked Parasite Development in the Mosquito Gut Frontiers in Microbiology plasmodium malaria TAtD-like DNase antibody transmission blocking vaccine |
| title | Plasmodium TatD-Like DNase Antibodies Blocked Parasite Development in the Mosquito Gut |
| title_full | Plasmodium TatD-Like DNase Antibodies Blocked Parasite Development in the Mosquito Gut |
| title_fullStr | Plasmodium TatD-Like DNase Antibodies Blocked Parasite Development in the Mosquito Gut |
| title_full_unstemmed | Plasmodium TatD-Like DNase Antibodies Blocked Parasite Development in the Mosquito Gut |
| title_short | Plasmodium TatD-Like DNase Antibodies Blocked Parasite Development in the Mosquito Gut |
| title_sort | plasmodium tatd like dnase antibodies blocked parasite development in the mosquito gut |
| topic | plasmodium malaria TAtD-like DNase antibody transmission blocking vaccine |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.01023/full |
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