Evaluation of optimum concentration of naphthalene acetic acid on <i>in vitro</i> rooting and acclimatization of tissue culture date palm (<i>Phoenix dactylifera</i> L.) plantlets
A major limitation in large scale application of micropropagation technology is high mortality experienced by in vitro raised plants during laboratory to land transfer. This study was aimed at investigating the best concentration of Naphthalene acetic acid (NAA) on in vitro rooting and also develop...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Joint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP)
2018-11-01
|
| Series: | Journal of Applied Sciences and Environmental Management |
| Subjects: | |
| Online Access: | https://www.ajol.info/index.php/jasem/article/view/180213 |
| _version_ | 1797227984915005440 |
|---|---|
| author | B.O. Emoghene O Asemota C.R. Eke M Idu E Aghimien A.P. Nwite |
| author_facet | B.O. Emoghene O Asemota C.R. Eke M Idu E Aghimien A.P. Nwite |
| author_sort | B.O. Emoghene |
| collection | DOAJ |
| description |
A major limitation in large scale application of micropropagation technology is high mortality experienced by in vitro raised plants during laboratory to land transfer. This study was aimed at investigating the best concentration of Naphthalene acetic acid (NAA) on in vitro rooting and also develop protocol for successful plant acclimatization with high survival rate. Date palm plantlets were rooted on Murashige and Skoog (MS) basal medium with different NAA concentrations (0.0, 0.1, 0.5 1.0, 1.5 and 2 mg/l) without activated charcoal. Result showed that all the NAA concentrations supported root formation. The optimal initiation and growth of roots of the plantlets were observed at NAA concentration of 1.0 mg/l (87 %) and least with Basal MS without NAA (47 %). Results also showed that root number and lengths increased with increasing NAA concentrations up to 1.0 mg/l and decreased thereafter. The culture mixture of peat moss + soil (2:1) gave the highest survival percentage of 80 % for plant acclimatization. This investigation had shown that accurate assessments of responses to medium at various stages should be considered as specific requirement for successful plant acclimatization.
Keywords: date palm, in vitro, plantlets, acclimatization
|
| first_indexed | 2024-04-24T14:49:29Z |
| format | Article |
| id | doaj.art-af0c4c3409dd44e697f0c2243df67ed2 |
| institution | Directory Open Access Journal |
| issn | 2659-1502 2659-1499 |
| language | English |
| last_indexed | 2024-04-24T14:49:29Z |
| publishDate | 2018-11-01 |
| publisher | Joint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP) |
| record_format | Article |
| series | Journal of Applied Sciences and Environmental Management |
| spelling | doaj.art-af0c4c3409dd44e697f0c2243df67ed22024-04-02T19:51:27ZengJoint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP)Journal of Applied Sciences and Environmental Management2659-15022659-14992018-11-01221010.4314/jasem.v22i10.11Evaluation of optimum concentration of naphthalene acetic acid on <i>in vitro</i> rooting and acclimatization of tissue culture date palm (<i>Phoenix dactylifera</i> L.) plantletsB.O. EmogheneO AsemotaC.R. EkeM IduE AghimienA.P. Nwite A major limitation in large scale application of micropropagation technology is high mortality experienced by in vitro raised plants during laboratory to land transfer. This study was aimed at investigating the best concentration of Naphthalene acetic acid (NAA) on in vitro rooting and also develop protocol for successful plant acclimatization with high survival rate. Date palm plantlets were rooted on Murashige and Skoog (MS) basal medium with different NAA concentrations (0.0, 0.1, 0.5 1.0, 1.5 and 2 mg/l) without activated charcoal. Result showed that all the NAA concentrations supported root formation. The optimal initiation and growth of roots of the plantlets were observed at NAA concentration of 1.0 mg/l (87 %) and least with Basal MS without NAA (47 %). Results also showed that root number and lengths increased with increasing NAA concentrations up to 1.0 mg/l and decreased thereafter. The culture mixture of peat moss + soil (2:1) gave the highest survival percentage of 80 % for plant acclimatization. This investigation had shown that accurate assessments of responses to medium at various stages should be considered as specific requirement for successful plant acclimatization. Keywords: date palm, in vitro, plantlets, acclimatization https://www.ajol.info/index.php/jasem/article/view/180213date palmin vitroplantletsacclimatization |
| spellingShingle | B.O. Emoghene O Asemota C.R. Eke M Idu E Aghimien A.P. Nwite Evaluation of optimum concentration of naphthalene acetic acid on <i>in vitro</i> rooting and acclimatization of tissue culture date palm (<i>Phoenix dactylifera</i> L.) plantlets Journal of Applied Sciences and Environmental Management date palm in vitro plantlets acclimatization |
| title | Evaluation of optimum concentration of naphthalene acetic acid on <i>in vitro</i> rooting and acclimatization of tissue culture date palm (<i>Phoenix dactylifera</i> L.) plantlets |
| title_full | Evaluation of optimum concentration of naphthalene acetic acid on <i>in vitro</i> rooting and acclimatization of tissue culture date palm (<i>Phoenix dactylifera</i> L.) plantlets |
| title_fullStr | Evaluation of optimum concentration of naphthalene acetic acid on <i>in vitro</i> rooting and acclimatization of tissue culture date palm (<i>Phoenix dactylifera</i> L.) plantlets |
| title_full_unstemmed | Evaluation of optimum concentration of naphthalene acetic acid on <i>in vitro</i> rooting and acclimatization of tissue culture date palm (<i>Phoenix dactylifera</i> L.) plantlets |
| title_short | Evaluation of optimum concentration of naphthalene acetic acid on <i>in vitro</i> rooting and acclimatization of tissue culture date palm (<i>Phoenix dactylifera</i> L.) plantlets |
| title_sort | evaluation of optimum concentration of naphthalene acetic acid on i in vitro i rooting and acclimatization of tissue culture date palm i phoenix dactylifera i l plantlets |
| topic | date palm in vitro plantlets acclimatization |
| url | https://www.ajol.info/index.php/jasem/article/view/180213 |
| work_keys_str_mv | AT boemoghene evaluationofoptimumconcentrationofnaphthaleneaceticacidoniinvitroirootingandacclimatizationoftissueculturedatepalmiphoenixdactyliferailplantlets AT oasemota evaluationofoptimumconcentrationofnaphthaleneaceticacidoniinvitroirootingandacclimatizationoftissueculturedatepalmiphoenixdactyliferailplantlets AT creke evaluationofoptimumconcentrationofnaphthaleneaceticacidoniinvitroirootingandacclimatizationoftissueculturedatepalmiphoenixdactyliferailplantlets AT midu evaluationofoptimumconcentrationofnaphthaleneaceticacidoniinvitroirootingandacclimatizationoftissueculturedatepalmiphoenixdactyliferailplantlets AT eaghimien evaluationofoptimumconcentrationofnaphthaleneaceticacidoniinvitroirootingandacclimatizationoftissueculturedatepalmiphoenixdactyliferailplantlets AT apnwite evaluationofoptimumconcentrationofnaphthaleneaceticacidoniinvitroirootingandacclimatizationoftissueculturedatepalmiphoenixdactyliferailplantlets |