The promoter region of lapA and its transcriptional regulation by Fis in Pseudomonas putida.
LapA is the biggest protein in Pseudomonas putida and a key factor for biofilm formation. Its importance and posttranslational regulation is rather thoroughly studied but less is known about the transcriptional regulation. Here we give evidence that transcription of lapA in LB-grown bacteria is init...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5612765?pdf=render |
| _version_ | 1798044869272272896 |
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| author | Hanna Ainelo Andrio Lahesaare Annika Teppo Maia Kivisaar Riho Teras |
| author_facet | Hanna Ainelo Andrio Lahesaare Annika Teppo Maia Kivisaar Riho Teras |
| author_sort | Hanna Ainelo |
| collection | DOAJ |
| description | LapA is the biggest protein in Pseudomonas putida and a key factor for biofilm formation. Its importance and posttranslational regulation is rather thoroughly studied but less is known about the transcriptional regulation. Here we give evidence that transcription of lapA in LB-grown bacteria is initiated from six promoters, three of which display moderate RpoS-dependence. The global transcription regulator Fis binds to the lapA promoter area at six positions in vitro, and Fis activates the transcription of lapA while overexpressed in cells. Two of the six Fis binding sites, Fis-A7 and Fis-A5, are necessary for the positive effect of Fis on the transcription of lapA in vivo. Our results indicate that Fis binding to the Fis-A7 site increases the level of transcription from the most distal promoter of lapA, whereas Fis binding to the Fis-A5 site could be important for modifying the promoter area topology. |
| first_indexed | 2024-04-11T23:11:12Z |
| format | Article |
| id | doaj.art-af0d4a84e72e411e9e0b456a3524f468 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-11T23:11:12Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-af0d4a84e72e411e9e0b456a3524f4682022-12-22T03:57:50ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01129e018548210.1371/journal.pone.0185482The promoter region of lapA and its transcriptional regulation by Fis in Pseudomonas putida.Hanna AineloAndrio LahesaareAnnika TeppoMaia KivisaarRiho TerasLapA is the biggest protein in Pseudomonas putida and a key factor for biofilm formation. Its importance and posttranslational regulation is rather thoroughly studied but less is known about the transcriptional regulation. Here we give evidence that transcription of lapA in LB-grown bacteria is initiated from six promoters, three of which display moderate RpoS-dependence. The global transcription regulator Fis binds to the lapA promoter area at six positions in vitro, and Fis activates the transcription of lapA while overexpressed in cells. Two of the six Fis binding sites, Fis-A7 and Fis-A5, are necessary for the positive effect of Fis on the transcription of lapA in vivo. Our results indicate that Fis binding to the Fis-A7 site increases the level of transcription from the most distal promoter of lapA, whereas Fis binding to the Fis-A5 site could be important for modifying the promoter area topology.http://europepmc.org/articles/PMC5612765?pdf=render |
| spellingShingle | Hanna Ainelo Andrio Lahesaare Annika Teppo Maia Kivisaar Riho Teras The promoter region of lapA and its transcriptional regulation by Fis in Pseudomonas putida. PLoS ONE |
| title | The promoter region of lapA and its transcriptional regulation by Fis in Pseudomonas putida. |
| title_full | The promoter region of lapA and its transcriptional regulation by Fis in Pseudomonas putida. |
| title_fullStr | The promoter region of lapA and its transcriptional regulation by Fis in Pseudomonas putida. |
| title_full_unstemmed | The promoter region of lapA and its transcriptional regulation by Fis in Pseudomonas putida. |
| title_short | The promoter region of lapA and its transcriptional regulation by Fis in Pseudomonas putida. |
| title_sort | promoter region of lapa and its transcriptional regulation by fis in pseudomonas putida |
| url | http://europepmc.org/articles/PMC5612765?pdf=render |
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