A Novel Method to Detect 3D Mandibular Changes Related to Soft-Diet Feeding
Craniofacial morphology varies among individuals, which is regulated by the interaction between genes and the environment. Soft-diet feeding is a widely-used experimental model for studying the association between the skeletal morphology and muscle-related loading on the bone. Traditionally, these s...
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| Format: | Article |
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Frontiers Media S.A.
2017-08-01
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| Series: | Frontiers in Physiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fphys.2017.00567/full |
| _version_ | 1819181249439203328 |
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| author | Kana Kono Chihiro Tanikawa Takeshi Yanagita Hiroshi Kamioka Takashi Yamashiro |
| author_facet | Kana Kono Chihiro Tanikawa Takeshi Yanagita Hiroshi Kamioka Takashi Yamashiro |
| author_sort | Kana Kono |
| collection | DOAJ |
| description | Craniofacial morphology varies among individuals, which is regulated by the interaction between genes and the environment. Soft-diet feeding is a widely-used experimental model for studying the association between the skeletal morphology and muscle-related loading on the bone. Traditionally, these studies have been based on linear and angular measurements provided on two-dimensional (2D) radiographs in the lateral view. However, 2D observation is based on simplification of the anatomical structures and cannot detect three-dimensional (3D) changes in detail. In this study, we newly developed a modified surface-based analysis with micro-3D computed tomography (CT) to examine and detect the 3D changes in the mandible associated with soft-diet feeding. Mice at 3 weeks of age were fed a powdered soft-diet (SD) or hard-diet (HD) of regular rodent pellets until 9 weeks of age. Micro-CT images were taken at age 9 weeks to reconstruct the anatomical architecture images. A computer-generated averaged mandible was superimposed to directly visualize the morphological phenotypes. Gross observation revealed the apparent changes at the posterior body of the mandible, the angular process and the condyle between HD and SD mice. Significant differences in the mapping indicated the regions of significant displacement in the SD mice over the averaged 3D image of the HD mice. This map revealed that vertical displacement was most evident in 3D changes. We also noted a combination of vertical, transverse and anteroposterior directions of displacement in the condylar growth, resulting in complicated shape changes in the whole condylar process in SD mice. In contrast, transverse displacement was more significant in the coronoid process. The map analysis further showed the significant outward displacement of the inner surface of the alveolar process, which consequently resulted in thinning of the alveolar process. |
| first_indexed | 2024-12-22T22:27:14Z |
| format | Article |
| id | doaj.art-af0e24e294cd499d84d52679ac9d2cdc |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-22T22:27:14Z |
| publishDate | 2017-08-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Physiology |
| spelling | doaj.art-af0e24e294cd499d84d52679ac9d2cdc2022-12-21T18:10:32ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2017-08-01810.3389/fphys.2017.00567283235A Novel Method to Detect 3D Mandibular Changes Related to Soft-Diet FeedingKana Kono0Chihiro Tanikawa1Takeshi Yanagita2Hiroshi Kamioka3Takashi Yamashiro4Department of Orthodontics, Okayama University HospitalOkayama, JapanDepartment of Orthodontics and Dentofacial Orthopedics, Graduate School of Dentistry, Osaka UniversityOsaka, JapanDepartment of Orthodontics, Okayama University HospitalOkayama, JapanGraduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityOkayama, JapanDepartment of Orthodontics and Dentofacial Orthopedics, Graduate School of Dentistry, Osaka UniversityOsaka, JapanCraniofacial morphology varies among individuals, which is regulated by the interaction between genes and the environment. Soft-diet feeding is a widely-used experimental model for studying the association between the skeletal morphology and muscle-related loading on the bone. Traditionally, these studies have been based on linear and angular measurements provided on two-dimensional (2D) radiographs in the lateral view. However, 2D observation is based on simplification of the anatomical structures and cannot detect three-dimensional (3D) changes in detail. In this study, we newly developed a modified surface-based analysis with micro-3D computed tomography (CT) to examine and detect the 3D changes in the mandible associated with soft-diet feeding. Mice at 3 weeks of age were fed a powdered soft-diet (SD) or hard-diet (HD) of regular rodent pellets until 9 weeks of age. Micro-CT images were taken at age 9 weeks to reconstruct the anatomical architecture images. A computer-generated averaged mandible was superimposed to directly visualize the morphological phenotypes. Gross observation revealed the apparent changes at the posterior body of the mandible, the angular process and the condyle between HD and SD mice. Significant differences in the mapping indicated the regions of significant displacement in the SD mice over the averaged 3D image of the HD mice. This map revealed that vertical displacement was most evident in 3D changes. We also noted a combination of vertical, transverse and anteroposterior directions of displacement in the condylar growth, resulting in complicated shape changes in the whole condylar process in SD mice. In contrast, transverse displacement was more significant in the coronoid process. The map analysis further showed the significant outward displacement of the inner surface of the alveolar process, which consequently resulted in thinning of the alveolar process.http://journal.frontiersin.org/article/10.3389/fphys.2017.00567/full3DCTsoft foodmicemandiblemorphological change |
| spellingShingle | Kana Kono Chihiro Tanikawa Takeshi Yanagita Hiroshi Kamioka Takashi Yamashiro A Novel Method to Detect 3D Mandibular Changes Related to Soft-Diet Feeding Frontiers in Physiology 3D CT soft food mice mandible morphological change |
| title | A Novel Method to Detect 3D Mandibular Changes Related to Soft-Diet Feeding |
| title_full | A Novel Method to Detect 3D Mandibular Changes Related to Soft-Diet Feeding |
| title_fullStr | A Novel Method to Detect 3D Mandibular Changes Related to Soft-Diet Feeding |
| title_full_unstemmed | A Novel Method to Detect 3D Mandibular Changes Related to Soft-Diet Feeding |
| title_short | A Novel Method to Detect 3D Mandibular Changes Related to Soft-Diet Feeding |
| title_sort | novel method to detect 3d mandibular changes related to soft diet feeding |
| topic | 3D CT soft food mice mandible morphological change |
| url | http://journal.frontiersin.org/article/10.3389/fphys.2017.00567/full |
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