A simple, non‐toxic method for separating seeds based on density, and its application in isolating Arabidopsis thaliana seed oil mutants
Premise Seed oil is an economically important trait in Brassica oilseed crops. A novel method was developed to isolate Arabidopsis thaliana seeds with altered oil content. Methods and Results In A. thaliana, seed oil content is correlated with seed density, with high‐oil seeds being less dense than...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-04-01
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| Series: | Applications in Plant Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/aps3.11332 |
| _version_ | 1818873155606806528 |
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| author | Gillian H. Dean Flora Pang George W. Haughn Ljerka Kunst |
| author_facet | Gillian H. Dean Flora Pang George W. Haughn Ljerka Kunst |
| author_sort | Gillian H. Dean |
| collection | DOAJ |
| description | Premise Seed oil is an economically important trait in Brassica oilseed crops. A novel method was developed to isolate Arabidopsis thaliana seeds with altered oil content. Methods and Results In A. thaliana, seed oil content is correlated with seed density, with high‐oil seeds being less dense than wild type and tending to float in solution, and low‐oil seeds being denser and tending to sink. In contrast to previous methods, which used toxic chemicals and density gradient centrifugation, different concentrations of calcium chloride (CaCl2) were employed to separate seeds without the need for centrifugation. The method was validated using known seed oil mutants, and 120,822 T‐DNA mutagenized A. thaliana lines were then screened for novel seed density phenotypes. Conclusions A number of candidate mutants, as well as new alleles of two genes known to influence seed oil biosynthesis, were successfully isolated. |
| first_indexed | 2024-12-19T12:50:13Z |
| format | Article |
| id | doaj.art-af0f151beac6465cb59bcd85fddf118f |
| institution | Directory Open Access Journal |
| issn | 2168-0450 |
| language | English |
| last_indexed | 2024-12-19T12:50:13Z |
| publishDate | 2020-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applications in Plant Sciences |
| spelling | doaj.art-af0f151beac6465cb59bcd85fddf118f2022-12-21T20:20:35ZengWileyApplications in Plant Sciences2168-04502020-04-0184n/an/a10.1002/aps3.11332A simple, non‐toxic method for separating seeds based on density, and its application in isolating Arabidopsis thaliana seed oil mutantsGillian H. Dean0Flora Pang1George W. Haughn2Ljerka Kunst3Department of Botany University of British Columbia 6270 University Boulevard Vancouver V6T 1Z4 CanadaDepartment of Botany University of British Columbia 6270 University Boulevard Vancouver V6T 1Z4 CanadaDepartment of Botany University of British Columbia 6270 University Boulevard Vancouver V6T 1Z4 CanadaDepartment of Botany University of British Columbia 6270 University Boulevard Vancouver V6T 1Z4 CanadaPremise Seed oil is an economically important trait in Brassica oilseed crops. A novel method was developed to isolate Arabidopsis thaliana seeds with altered oil content. Methods and Results In A. thaliana, seed oil content is correlated with seed density, with high‐oil seeds being less dense than wild type and tending to float in solution, and low‐oil seeds being denser and tending to sink. In contrast to previous methods, which used toxic chemicals and density gradient centrifugation, different concentrations of calcium chloride (CaCl2) were employed to separate seeds without the need for centrifugation. The method was validated using known seed oil mutants, and 120,822 T‐DNA mutagenized A. thaliana lines were then screened for novel seed density phenotypes. Conclusions A number of candidate mutants, as well as new alleles of two genes known to influence seed oil biosynthesis, were successfully isolated.https://doi.org/10.1002/aps3.11332Arabidopsis thalianaseed densityseed oilseed separation |
| spellingShingle | Gillian H. Dean Flora Pang George W. Haughn Ljerka Kunst A simple, non‐toxic method for separating seeds based on density, and its application in isolating Arabidopsis thaliana seed oil mutants Applications in Plant Sciences Arabidopsis thaliana seed density seed oil seed separation |
| title | A simple, non‐toxic method for separating seeds based on density, and its application in isolating Arabidopsis thaliana seed oil mutants |
| title_full | A simple, non‐toxic method for separating seeds based on density, and its application in isolating Arabidopsis thaliana seed oil mutants |
| title_fullStr | A simple, non‐toxic method for separating seeds based on density, and its application in isolating Arabidopsis thaliana seed oil mutants |
| title_full_unstemmed | A simple, non‐toxic method for separating seeds based on density, and its application in isolating Arabidopsis thaliana seed oil mutants |
| title_short | A simple, non‐toxic method for separating seeds based on density, and its application in isolating Arabidopsis thaliana seed oil mutants |
| title_sort | simple non toxic method for separating seeds based on density and its application in isolating arabidopsis thaliana seed oil mutants |
| topic | Arabidopsis thaliana seed density seed oil seed separation |
| url | https://doi.org/10.1002/aps3.11332 |
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