Engineering the Active Site Lid Dynamics to Improve the Catalytic Efficiency of Yeast Cytosine Deaminase

Engineering the Active Site Lid Dynamics to Improve the Catalytic Efficiency of Yeast Cytosine Deaminase

Conformational dynamics is important for enzyme catalysis. However, engineering dynamics to achieve a higher catalytic efficiency is still challenging. In this work, we develop a new strategy to improve the activity of yeast cytosine deaminase (yCD) by engineering its conformational dynamics. Specif...

Full description

Bibliographic Details
Main Authors: Hanzhong Deng, Mingming Qin, Zhijun Liu, Ying Yang, Yefei Wang, Lishan Yao
Format: Article
Language:English
Published: MDPI AG 2023-04-01
Series:International Journal of Molecular Sciences
Subjects:
dynamics engineering
cytosine deaminase
prodrug
protein structure
Online Access:https://www.mdpi.com/1422-0067/24/7/6592

Similar Items

Similar Items