Histochemical and biometric study of the gastrointestinal system of Hyla orientalis (Bedriaga, 1890) (Anura, Hylidae)
<p class="Default">This study was carried out to assess the localization of hyaluronic acid (HA) and the distribution of glycoproteins in the gastrointestinal system of adult <em>Hyla orientalis</em>. Histochemical analysis of the gastrointestinal system in <em>H. o...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PAGEPress Publications
2014-10-01
|
| Series: | European Journal of Histochemistry |
| Subjects: | |
| Online Access: | http://www.ejh.it/index.php/ejh/article/view/2452 |
| _version_ | 1819028639837061120 |
|---|---|
| author | E. Akat H. Arıkan B. Göçmen |
| author_facet | E. Akat H. Arıkan B. Göçmen |
| author_sort | E. Akat |
| collection | DOAJ |
| description | <p class="Default">This study was carried out to assess the localization of hyaluronic acid (HA) and the distribution of glycoproteins in the gastrointestinal system of adult <em>Hyla orientalis</em>. Histochemical analysis of the gastrointestinal system in <em>H. orientalis </em>showed that mucous content included glycogene and/or oxidable dioles [periodic acid/Schiff (PAS)+], neutral or acid-rich (PAS/AB pH 2.5+), sialic acid residues (KOH/PAS+) and acid sulphate [Aldehyde fuchsin (AF)+] glycoproteins. However the mucus content was not the same in stomach, small and large intestine. The mucus content of stomach included only glycogene and/or oxidable dioles and sialic acid residues. Besides these histochemical methods, the localization of HA was detected using biotinylated hyaluronic acid binding protein labeled with streptavidin-fluorescein isothiocyanate (FITC). In the extracellular matrix of the submucosa, the reaction for HA was evident. Since HA was located in submucosa beneath the epithelial layer of gastrointestinal system, it has a significant role in hydric balance, and essential to provide the gastrointestinal system integrity and functionality. According to biometric results, there were statistical differences between small and large intestine in terms of the amount of material stained positive with PAS/AB, PAS, KOH/PAS and AF/AB. Additionally, number of goblet cells in the small and large intestine was significantly different.</p> |
| first_indexed | 2024-12-21T06:01:34Z |
| format | Article |
| id | doaj.art-af1620fe6c97443abc00b95d05bcb1d4 |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-12-21T06:01:34Z |
| publishDate | 2014-10-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-af1620fe6c97443abc00b95d05bcb1d42022-12-21T19:13:45ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062014-10-0158410.4081/ejh.2014.24521426Histochemical and biometric study of the gastrointestinal system of Hyla orientalis (Bedriaga, 1890) (Anura, Hylidae)E. Akat0H. Arıkan1B. Göçmen2Ege UniversityEge UniversityEge University<p class="Default">This study was carried out to assess the localization of hyaluronic acid (HA) and the distribution of glycoproteins in the gastrointestinal system of adult <em>Hyla orientalis</em>. Histochemical analysis of the gastrointestinal system in <em>H. orientalis </em>showed that mucous content included glycogene and/or oxidable dioles [periodic acid/Schiff (PAS)+], neutral or acid-rich (PAS/AB pH 2.5+), sialic acid residues (KOH/PAS+) and acid sulphate [Aldehyde fuchsin (AF)+] glycoproteins. However the mucus content was not the same in stomach, small and large intestine. The mucus content of stomach included only glycogene and/or oxidable dioles and sialic acid residues. Besides these histochemical methods, the localization of HA was detected using biotinylated hyaluronic acid binding protein labeled with streptavidin-fluorescein isothiocyanate (FITC). In the extracellular matrix of the submucosa, the reaction for HA was evident. Since HA was located in submucosa beneath the epithelial layer of gastrointestinal system, it has a significant role in hydric balance, and essential to provide the gastrointestinal system integrity and functionality. According to biometric results, there were statistical differences between small and large intestine in terms of the amount of material stained positive with PAS/AB, PAS, KOH/PAS and AF/AB. Additionally, number of goblet cells in the small and large intestine was significantly different.</p>http://www.ejh.it/index.php/ejh/article/view/2452Gastrointestinal systemgoblet cellglycoproteinshyaluronic acidamphibianHyla. |
| spellingShingle | E. Akat H. Arıkan B. Göçmen Histochemical and biometric study of the gastrointestinal system of Hyla orientalis (Bedriaga, 1890) (Anura, Hylidae) European Journal of Histochemistry Gastrointestinal system goblet cell glycoproteins hyaluronic acid amphibian Hyla. |
| title | Histochemical and biometric study of the gastrointestinal system of Hyla orientalis (Bedriaga, 1890) (Anura, Hylidae) |
| title_full | Histochemical and biometric study of the gastrointestinal system of Hyla orientalis (Bedriaga, 1890) (Anura, Hylidae) |
| title_fullStr | Histochemical and biometric study of the gastrointestinal system of Hyla orientalis (Bedriaga, 1890) (Anura, Hylidae) |
| title_full_unstemmed | Histochemical and biometric study of the gastrointestinal system of Hyla orientalis (Bedriaga, 1890) (Anura, Hylidae) |
| title_short | Histochemical and biometric study of the gastrointestinal system of Hyla orientalis (Bedriaga, 1890) (Anura, Hylidae) |
| title_sort | histochemical and biometric study of the gastrointestinal system of hyla orientalis bedriaga 1890 anura hylidae |
| topic | Gastrointestinal system goblet cell glycoproteins hyaluronic acid amphibian Hyla. |
| url | http://www.ejh.it/index.php/ejh/article/view/2452 |
| work_keys_str_mv | AT eakat histochemicalandbiometricstudyofthegastrointestinalsystemofhylaorientalisbedriaga1890anurahylidae AT harıkan histochemicalandbiometricstudyofthegastrointestinalsystemofhylaorientalisbedriaga1890anurahylidae AT bgocmen histochemicalandbiometricstudyofthegastrointestinalsystemofhylaorientalisbedriaga1890anurahylidae |