Eco-friendly RP-HPLC method for determination of diazepam in coated tablet
A new eco-friendly RP-HPLC method for determining diazepam in coated tablets was developed and validated. The separation was achieved on a Lichrosper® 100 RP-18 (250 mm × 4 mm, 5 µm particle size) using the isocratic elution mode with a mobile phase composed of a mixture of water (H2O) and ethanol...
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| Format: | Article |
| Language: | English |
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Society of Chemists and Technologists of Macedonia
2023-11-01
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| Series: | Macedonian Journal of Chemistry and Chemical Engineering |
| Subjects: | |
| Online Access: | https://mjcce.org.mk/index.php/MJCCE/article/view/2683 |
| _version_ | 1797423441021763584 |
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| author | Marija Tomić Marijana Božinovska Nataša Anevska-Stojanovska Jelena Lazova Rumenka Petkovska Liljana Anastasova Natalija Nakov |
| author_facet | Marija Tomić Marijana Božinovska Nataša Anevska-Stojanovska Jelena Lazova Rumenka Petkovska Liljana Anastasova Natalija Nakov |
| author_sort | Marija Tomić |
| collection | DOAJ |
| description |
A new eco-friendly RP-HPLC method for determining diazepam in coated tablets was developed and validated. The separation was achieved on a Lichrosper® 100 RP-18 (250 mm × 4 mm, 5 µm particle size) using the isocratic elution mode with a mobile phase composed of a mixture of water (H2O) and ethanol (EtOH) in a 40:60 (v/v %) ratio and a flow rate of 1.0 mL/min. The injection volume was 10 μL, and the detection wavelength was set at 254 nm. The column temperature was maintained at 35 ºC. The method was validated according to the ICH guideline, emphasizing selectivity/specificity, linearity, sensitivity, accuracy, precision, and robustness. The analytical method greenness score and Eco-scale approach were used for the method’s greenness assessment. The method was applied to determine the assay of diazepam in coated tablets and the uniformity of dosage units by content uniformity.
Applying the method proposed in this study in the pharmaceutical industry is considered environmentally sustainable. It would bring benefits in terms of a safer working environment and reduction of toxic waste formation without compromising the reliability of the analytical results.
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| first_indexed | 2024-03-09T07:47:00Z |
| format | Article |
| id | doaj.art-af199099ed2c4ca9b31c64a5050e47b2 |
| institution | Directory Open Access Journal |
| issn | 1857-5552 1857-5625 |
| language | English |
| last_indexed | 2024-03-09T07:47:00Z |
| publishDate | 2023-11-01 |
| publisher | Society of Chemists and Technologists of Macedonia |
| record_format | Article |
| series | Macedonian Journal of Chemistry and Chemical Engineering |
| spelling | doaj.art-af199099ed2c4ca9b31c64a5050e47b22023-12-03T02:58:54ZengSociety of Chemists and Technologists of MacedoniaMacedonian Journal of Chemistry and Chemical Engineering1857-55521857-56252023-11-0142210.20450/mjcce.2023.2683Eco-friendly RP-HPLC method for determination of diazepam in coated tabletMarija Tomić0Marijana Božinovska1Nataša Anevska-Stojanovska2Jelena Lazova3Rumenka Petkovska4Liljana Anastasova5Natalija Nakov6Research & Development, ALKALOID AD SkopjeResearch & Development, ALKALOID AD SkopjeResearch & Development, ALKALOID AD SkopjeResearch & Development, ALKALOID AD SkopjeFaculty of Pharmacy, Ss. Cyril and Methodius University in SkopjeFaculty of Pharmacy, Ss. Cyril and Methodius University in SkopjeFaculty of Pharmacy, Ss. Cyril and Methodius University in Skopje A new eco-friendly RP-HPLC method for determining diazepam in coated tablets was developed and validated. The separation was achieved on a Lichrosper® 100 RP-18 (250 mm × 4 mm, 5 µm particle size) using the isocratic elution mode with a mobile phase composed of a mixture of water (H2O) and ethanol (EtOH) in a 40:60 (v/v %) ratio and a flow rate of 1.0 mL/min. The injection volume was 10 μL, and the detection wavelength was set at 254 nm. The column temperature was maintained at 35 ºC. The method was validated according to the ICH guideline, emphasizing selectivity/specificity, linearity, sensitivity, accuracy, precision, and robustness. The analytical method greenness score and Eco-scale approach were used for the method’s greenness assessment. The method was applied to determine the assay of diazepam in coated tablets and the uniformity of dosage units by content uniformity. Applying the method proposed in this study in the pharmaceutical industry is considered environmentally sustainable. It would bring benefits in terms of a safer working environment and reduction of toxic waste formation without compromising the reliability of the analytical results. https://mjcce.org.mk/index.php/MJCCE/article/view/2683green/eco-friendly analytical methodsreversed-phase liquid chromatographyethanoldiazepampharmaceutical analysis |
| spellingShingle | Marija Tomić Marijana Božinovska Nataša Anevska-Stojanovska Jelena Lazova Rumenka Petkovska Liljana Anastasova Natalija Nakov Eco-friendly RP-HPLC method for determination of diazepam in coated tablet Macedonian Journal of Chemistry and Chemical Engineering green/eco-friendly analytical methods reversed-phase liquid chromatography ethanol diazepam pharmaceutical analysis |
| title | Eco-friendly RP-HPLC method for determination of diazepam in coated tablet |
| title_full | Eco-friendly RP-HPLC method for determination of diazepam in coated tablet |
| title_fullStr | Eco-friendly RP-HPLC method for determination of diazepam in coated tablet |
| title_full_unstemmed | Eco-friendly RP-HPLC method for determination of diazepam in coated tablet |
| title_short | Eco-friendly RP-HPLC method for determination of diazepam in coated tablet |
| title_sort | eco friendly rp hplc method for determination of diazepam in coated tablet |
| topic | green/eco-friendly analytical methods reversed-phase liquid chromatography ethanol diazepam pharmaceutical analysis |
| url | https://mjcce.org.mk/index.php/MJCCE/article/view/2683 |
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