Mesenchymal Stem Cells Pretreated with Collagen Promote Skin Wound-Healing
The existing treatment modalities for skin injuries mainly include dressings, negative-pressure wound treatment, autologous skin grafting, and high-pressure wound treatment. All of these therapies have limitations such as high time cost, the inability to remove inactivated tissue in a timely manner,...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/10/8688 |
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| author | Zheng Kou Balun Li Aili Aierken Ning Tan Chenchen Li Miao Han Yuanxiang Jing Na Li Shiqiang Zhang Sha Peng Xianjun Zhao Jinlian Hua |
| author_facet | Zheng Kou Balun Li Aili Aierken Ning Tan Chenchen Li Miao Han Yuanxiang Jing Na Li Shiqiang Zhang Sha Peng Xianjun Zhao Jinlian Hua |
| author_sort | Zheng Kou |
| collection | DOAJ |
| description | The existing treatment modalities for skin injuries mainly include dressings, negative-pressure wound treatment, autologous skin grafting, and high-pressure wound treatment. All of these therapies have limitations such as high time cost, the inability to remove inactivated tissue in a timely manner, surgical debridement, and oxygen toxicity. Mesenchymal stem cells have a unique self-renewal ability and wide differentiation potential, and they are one of the most promising stem cell types in cell therapy and have great application prospects in the field of regenerative medicine. Collagen exerts structural roles by promoting the molecular structure, shape, and mechanical properties of cells, and adding it to cell cultures can also promote cell proliferation and shorten the cell doubling time. The effects of collagen on MSCs were examined using Giemsa staining, EdU staining, and growth curves. Mice were subjected to allogeneic experiments and autologous experiments to reduce individual differences; all animals were separated into four groups. Neonatal skin sections were detected by HE staining, Masson staining, immunohistochemical staining, and immunofluorescence staining. We found that the MSCs pretreated with collagen accelerated the healing of skin wounds in mice and canines by promoting epidermal layer repair, collagen deposition, hair follicle angiogenesis, and an inflammatory response. Collagen promotes the secretion of the chemokines and growth factors associated with skin healing by MSCs, which positively influences skin healing. This study supports the treatment of skin injuries with MSCs cultured in medium with collagen added. |
| first_indexed | 2024-03-11T03:40:49Z |
| format | Article |
| id | doaj.art-af19d63ec52f492ab2f30d5c0b9d0354 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T03:40:49Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-af19d63ec52f492ab2f30d5c0b9d03542023-11-18T01:39:59ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-05-012410868810.3390/ijms24108688Mesenchymal Stem Cells Pretreated with Collagen Promote Skin Wound-HealingZheng Kou0Balun Li1Aili Aierken2Ning Tan3Chenchen Li4Miao Han5Yuanxiang Jing6Na Li7Shiqiang Zhang8Sha Peng9Xianjun Zhao10Jinlian Hua11College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Northwest A & F University, Yangling, Xianyang 712100, ChinaCollege of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Northwest A & F University, Yangling, Xianyang 712100, ChinaCollege of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Northwest A & F University, Yangling, Xianyang 712100, ChinaCollege of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Northwest A & F University, Yangling, Xianyang 712100, ChinaCollege of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Northwest A & F University, Yangling, Xianyang 712100, ChinaCollege of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Northwest A & F University, Yangling, Xianyang 712100, ChinaCollege of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Northwest A & F University, Yangling, Xianyang 712100, ChinaCollege of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Northwest A & F University, Yangling, Xianyang 712100, ChinaCollege of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Northwest A & F University, Yangling, Xianyang 712100, ChinaCollege of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Northwest A & F University, Yangling, Xianyang 712100, ChinaCollege of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Northwest A & F University, Yangling, Xianyang 712100, ChinaCollege of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Northwest A & F University, Yangling, Xianyang 712100, ChinaThe existing treatment modalities for skin injuries mainly include dressings, negative-pressure wound treatment, autologous skin grafting, and high-pressure wound treatment. All of these therapies have limitations such as high time cost, the inability to remove inactivated tissue in a timely manner, surgical debridement, and oxygen toxicity. Mesenchymal stem cells have a unique self-renewal ability and wide differentiation potential, and they are one of the most promising stem cell types in cell therapy and have great application prospects in the field of regenerative medicine. Collagen exerts structural roles by promoting the molecular structure, shape, and mechanical properties of cells, and adding it to cell cultures can also promote cell proliferation and shorten the cell doubling time. The effects of collagen on MSCs were examined using Giemsa staining, EdU staining, and growth curves. Mice were subjected to allogeneic experiments and autologous experiments to reduce individual differences; all animals were separated into four groups. Neonatal skin sections were detected by HE staining, Masson staining, immunohistochemical staining, and immunofluorescence staining. We found that the MSCs pretreated with collagen accelerated the healing of skin wounds in mice and canines by promoting epidermal layer repair, collagen deposition, hair follicle angiogenesis, and an inflammatory response. Collagen promotes the secretion of the chemokines and growth factors associated with skin healing by MSCs, which positively influences skin healing. This study supports the treatment of skin injuries with MSCs cultured in medium with collagen added.https://www.mdpi.com/1422-0067/24/10/8688mesenchymal stem cellscollagenskin woundrepair |
| spellingShingle | Zheng Kou Balun Li Aili Aierken Ning Tan Chenchen Li Miao Han Yuanxiang Jing Na Li Shiqiang Zhang Sha Peng Xianjun Zhao Jinlian Hua Mesenchymal Stem Cells Pretreated with Collagen Promote Skin Wound-Healing International Journal of Molecular Sciences mesenchymal stem cells collagen skin wound repair |
| title | Mesenchymal Stem Cells Pretreated with Collagen Promote Skin Wound-Healing |
| title_full | Mesenchymal Stem Cells Pretreated with Collagen Promote Skin Wound-Healing |
| title_fullStr | Mesenchymal Stem Cells Pretreated with Collagen Promote Skin Wound-Healing |
| title_full_unstemmed | Mesenchymal Stem Cells Pretreated with Collagen Promote Skin Wound-Healing |
| title_short | Mesenchymal Stem Cells Pretreated with Collagen Promote Skin Wound-Healing |
| title_sort | mesenchymal stem cells pretreated with collagen promote skin wound healing |
| topic | mesenchymal stem cells collagen skin wound repair |
| url | https://www.mdpi.com/1422-0067/24/10/8688 |
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