Biochemical and hematological study of goats envenomed with natural and 60Co-irradiated bothropic venom
Venoms from snakes of the Bothrops genus are proteolytic, coagulant, hemorrhagic and nephrotoxic, causing edema, necrosis, hemorrhage and intense pain at the bite site, besides systemic alterations. Many adjuvants have been added to the venom used in the sensitization of antiserum-producer animals t...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SciELO
2007-01-01
|
| Series: | Journal of Venomous Animals and Toxins including Tropical Diseases |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000300003 |
| _version_ | 1828463444589805568 |
|---|---|
| author | P. C. Lucas de Oliveira M. Sakate R. A. Madruga N. P.U. Barbosa |
| author_facet | P. C. Lucas de Oliveira M. Sakate R. A. Madruga N. P.U. Barbosa |
| author_sort | P. C. Lucas de Oliveira |
| collection | DOAJ |
| description | Venoms from snakes of the Bothrops genus are proteolytic, coagulant, hemorrhagic and nephrotoxic, causing edema, necrosis, hemorrhage and intense pain at the bite site, besides systemic alterations. Many adjuvants have been added to the venom used in the sensitization of antiserum-producer animals to increase antigenic induction and reduce the envenomation pathological effects. Gamma radiation from 60Co has been used as an attenuating agent of the venoms toxic properties. The main objective was to study, comparatively, clinical and laboratory aspects of goats inoculated with bothropic (Bothrops jararaca) venom, natural and irradiated from a 60Co source. Twelve goats were divided into two groups of six animals: GINV, inoculated with 0.5mg/kg of natural venom; and GIIV, inoculated with 0.5mg/kg of irradiated venom. Blood samples were collected immediately before and one, two, seven, and thirty days after venom injection. Local lesions were daily evaluated. The following exams were carried out: blood tests; biochemical tests of urea, creatinine, creatine kinase (CK), aspartate amino-transferase (AST) and alanine amino-transferase (ALT); clotting time; platelets count; and total serum immunoglobulin measurement. In the conditions of the present experiment, irradiated venom was less aggressive and more immunogenic than natural venom. |
| first_indexed | 2024-12-11T02:53:53Z |
| format | Article |
| id | doaj.art-af1c559467344331ad4ab9f52371cebe |
| institution | Directory Open Access Journal |
| issn | 1678-9199 |
| language | English |
| last_indexed | 2024-12-11T02:53:53Z |
| publishDate | 2007-01-01 |
| publisher | SciELO |
| record_format | Article |
| series | Journal of Venomous Animals and Toxins including Tropical Diseases |
| spelling | doaj.art-af1c559467344331ad4ab9f52371cebe2022-12-22T01:23:13ZengSciELOJournal of Venomous Animals and Toxins including Tropical Diseases1678-91992007-01-0113357659710.1590/S1678-91992007000300003Biochemical and hematological study of goats envenomed with natural and 60Co-irradiated bothropic venomP. C. Lucas de OliveiraM. SakateR. A. MadrugaN. P.U. BarbosaVenoms from snakes of the Bothrops genus are proteolytic, coagulant, hemorrhagic and nephrotoxic, causing edema, necrosis, hemorrhage and intense pain at the bite site, besides systemic alterations. Many adjuvants have been added to the venom used in the sensitization of antiserum-producer animals to increase antigenic induction and reduce the envenomation pathological effects. Gamma radiation from 60Co has been used as an attenuating agent of the venoms toxic properties. The main objective was to study, comparatively, clinical and laboratory aspects of goats inoculated with bothropic (Bothrops jararaca) venom, natural and irradiated from a 60Co source. Twelve goats were divided into two groups of six animals: GINV, inoculated with 0.5mg/kg of natural venom; and GIIV, inoculated with 0.5mg/kg of irradiated venom. Blood samples were collected immediately before and one, two, seven, and thirty days after venom injection. Local lesions were daily evaluated. The following exams were carried out: blood tests; biochemical tests of urea, creatinine, creatine kinase (CK), aspartate amino-transferase (AST) and alanine amino-transferase (ALT); clotting time; platelets count; and total serum immunoglobulin measurement. In the conditions of the present experiment, irradiated venom was less aggressive and more immunogenic than natural venom.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000300003radiationBothrops jararacacobaltgoats |
| spellingShingle | P. C. Lucas de Oliveira M. Sakate R. A. Madruga N. P.U. Barbosa Biochemical and hematological study of goats envenomed with natural and 60Co-irradiated bothropic venom Journal of Venomous Animals and Toxins including Tropical Diseases radiation Bothrops jararaca cobalt goats |
| title | Biochemical and hematological study of goats envenomed with natural and 60Co-irradiated bothropic venom |
| title_full | Biochemical and hematological study of goats envenomed with natural and 60Co-irradiated bothropic venom |
| title_fullStr | Biochemical and hematological study of goats envenomed with natural and 60Co-irradiated bothropic venom |
| title_full_unstemmed | Biochemical and hematological study of goats envenomed with natural and 60Co-irradiated bothropic venom |
| title_short | Biochemical and hematological study of goats envenomed with natural and 60Co-irradiated bothropic venom |
| title_sort | biochemical and hematological study of goats envenomed with natural and 60co irradiated bothropic venom |
| topic | radiation Bothrops jararaca cobalt goats |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000300003 |
| work_keys_str_mv | AT pclucasdeoliveira biochemicalandhematologicalstudyofgoatsenvenomedwithnaturaland60coirradiatedbothropicvenom AT msakate biochemicalandhematologicalstudyofgoatsenvenomedwithnaturaland60coirradiatedbothropicvenom AT ramadruga biochemicalandhematologicalstudyofgoatsenvenomedwithnaturaland60coirradiatedbothropicvenom AT npubarbosa biochemicalandhematologicalstudyofgoatsenvenomedwithnaturaland60coirradiatedbothropicvenom |