MiR-1224 Acts as a Prognostic Biomarker and Inhibits the Progression of Gastric Cancer by Targeting SATB1
ObjectiveMiR-1224 has been reported to exhibit abnormal expression in several tumors. However, the expressing pattern and roles of miR-1224 in gastric cancer (GC) remain unclear. Our current research aimed to explore the potential involvement of miR-1224 in the GC progression.Materials and MethodsTh...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-09-01
|
| Series: | Frontiers in Oncology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2021.748896/full |
| _version_ | 1831586234609172480 |
|---|---|
| author | Guo-Dong Han Yuan Sun Hong-Xia Hui Ming-Yue Tao Yang-Qing Liu Jing Zhu |
| author_facet | Guo-Dong Han Yuan Sun Hong-Xia Hui Ming-Yue Tao Yang-Qing Liu Jing Zhu |
| author_sort | Guo-Dong Han |
| collection | DOAJ |
| description | ObjectiveMiR-1224 has been reported to exhibit abnormal expression in several tumors. However, the expressing pattern and roles of miR-1224 in gastric cancer (GC) remain unclear. Our current research aimed to explore the potential involvement of miR-1224 in the GC progression.Materials and MethodsThe expression of miR-1224 was examined in tissue samples of 128 GC patients and cell lines by RT-PCR. Besides, the associations of miR-1224 expressions with clinicopathologic features and prognosis of GC patients were analyzed. Then, the possible influences of miR-1224 on cell proliferation and cell migration were determined. Afterward, the molecular target of miR-1224 was identified using bioinformatics assays and confirmed experimentally. Finally, RT-PCR and Western blot assays were performed to investigate the effect of the abnormal miR-1224 expression on the EMT and Wnt/β-catenin pathway.ResultsmiR-1224 was lowly expressed in the GC specimens and cell lines due to T classification and TNM stage. Survival assays demonstrated that GC patients with low expressions of miR-1224 possessed poor overall survivals. Moreover, in vitro and in vivo assays revealed that the overexpression of miR-1224 inhibited cell proliferation, migration, and invasion in GC cells. SATB homeobox 1 (SATB1) was verified as a direct target of miR-1224 in GC. Furthermore, β-catenin and c-myc were significantly inhibited in miR-1224-overexpression cells.ConclusionsOur findings highlight the potential of miR-1224 as a therapeutic target and novel biomarker for GC patients |
| first_indexed | 2024-12-17T21:32:42Z |
| format | Article |
| id | doaj.art-af1cdf92ce2e4e0ca8c2482c033fd2b1 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-17T21:32:42Z |
| publishDate | 2021-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-af1cdf92ce2e4e0ca8c2482c033fd2b12022-12-21T21:31:50ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2021-09-011110.3389/fonc.2021.748896748896MiR-1224 Acts as a Prognostic Biomarker and Inhibits the Progression of Gastric Cancer by Targeting SATB1Guo-Dong Han0Yuan Sun1Hong-Xia Hui2Ming-Yue Tao3Yang-Qing Liu4Jing Zhu5Department of Orthopedics, The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical University, Huai’an, ChinaDepartment of Medical Oncology, The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical University, Huai’an, ChinaDepartment of Medical Oncology, The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical University, Huai’an, ChinaDepartment of Medical Oncology, The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical University, Huai’an, ChinaDepartment of Medical Oncology, The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical University, Huai’an, ChinaDepartment of Medical Oncology, The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical University, Huai’an, ChinaObjectiveMiR-1224 has been reported to exhibit abnormal expression in several tumors. However, the expressing pattern and roles of miR-1224 in gastric cancer (GC) remain unclear. Our current research aimed to explore the potential involvement of miR-1224 in the GC progression.Materials and MethodsThe expression of miR-1224 was examined in tissue samples of 128 GC patients and cell lines by RT-PCR. Besides, the associations of miR-1224 expressions with clinicopathologic features and prognosis of GC patients were analyzed. Then, the possible influences of miR-1224 on cell proliferation and cell migration were determined. Afterward, the molecular target of miR-1224 was identified using bioinformatics assays and confirmed experimentally. Finally, RT-PCR and Western blot assays were performed to investigate the effect of the abnormal miR-1224 expression on the EMT and Wnt/β-catenin pathway.ResultsmiR-1224 was lowly expressed in the GC specimens and cell lines due to T classification and TNM stage. Survival assays demonstrated that GC patients with low expressions of miR-1224 possessed poor overall survivals. Moreover, in vitro and in vivo assays revealed that the overexpression of miR-1224 inhibited cell proliferation, migration, and invasion in GC cells. SATB homeobox 1 (SATB1) was verified as a direct target of miR-1224 in GC. Furthermore, β-catenin and c-myc were significantly inhibited in miR-1224-overexpression cells.ConclusionsOur findings highlight the potential of miR-1224 as a therapeutic target and novel biomarker for GC patientshttps://www.frontiersin.org/articles/10.3389/fonc.2021.748896/fullmiR-1224metastasisSATB1Wnt/β-cateningastric cancerprognosis |
| spellingShingle | Guo-Dong Han Yuan Sun Hong-Xia Hui Ming-Yue Tao Yang-Qing Liu Jing Zhu MiR-1224 Acts as a Prognostic Biomarker and Inhibits the Progression of Gastric Cancer by Targeting SATB1 Frontiers in Oncology miR-1224 metastasis SATB1 Wnt/β-catenin gastric cancer prognosis |
| title | MiR-1224 Acts as a Prognostic Biomarker and Inhibits the Progression of Gastric Cancer by Targeting SATB1 |
| title_full | MiR-1224 Acts as a Prognostic Biomarker and Inhibits the Progression of Gastric Cancer by Targeting SATB1 |
| title_fullStr | MiR-1224 Acts as a Prognostic Biomarker and Inhibits the Progression of Gastric Cancer by Targeting SATB1 |
| title_full_unstemmed | MiR-1224 Acts as a Prognostic Biomarker and Inhibits the Progression of Gastric Cancer by Targeting SATB1 |
| title_short | MiR-1224 Acts as a Prognostic Biomarker and Inhibits the Progression of Gastric Cancer by Targeting SATB1 |
| title_sort | mir 1224 acts as a prognostic biomarker and inhibits the progression of gastric cancer by targeting satb1 |
| topic | miR-1224 metastasis SATB1 Wnt/β-catenin gastric cancer prognosis |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2021.748896/full |
| work_keys_str_mv | AT guodonghan mir1224actsasaprognosticbiomarkerandinhibitstheprogressionofgastriccancerbytargetingsatb1 AT yuansun mir1224actsasaprognosticbiomarkerandinhibitstheprogressionofgastriccancerbytargetingsatb1 AT hongxiahui mir1224actsasaprognosticbiomarkerandinhibitstheprogressionofgastriccancerbytargetingsatb1 AT mingyuetao mir1224actsasaprognosticbiomarkerandinhibitstheprogressionofgastriccancerbytargetingsatb1 AT yangqingliu mir1224actsasaprognosticbiomarkerandinhibitstheprogressionofgastriccancerbytargetingsatb1 AT jingzhu mir1224actsasaprognosticbiomarkerandinhibitstheprogressionofgastriccancerbytargetingsatb1 |