The Role of Probiotic Bacillus Spores and Amino Acids with Immunoglobulins on a Rat Enteropathy Model
Non-steroidal anti-inflammatory drugs (NSAIDs) are some of the most widely used drugs due to their anti-inflammatory, analgesic and antipyretic pharmacological effects. Gastrointestinal side effects are some of the most severe and frequent side effects of NSAIDs. These depend on the balance of the g...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-10-01
|
| Series: | Biomedicines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9059/10/10/2508 |
| _version_ | 1827651400932261888 |
|---|---|
| author | Maria-Adriana Neag Carmen-Stanca Melincovici Adrian Catinean Dana-Maria Muntean Raluca-Maria Pop Ioana-Corina Bocsan Andrei-Otto Mitre Mihai-Bogdan Cardos Andreea-Ioana Inceu Anca-Dana Buzoianu |
| author_facet | Maria-Adriana Neag Carmen-Stanca Melincovici Adrian Catinean Dana-Maria Muntean Raluca-Maria Pop Ioana-Corina Bocsan Andrei-Otto Mitre Mihai-Bogdan Cardos Andreea-Ioana Inceu Anca-Dana Buzoianu |
| author_sort | Maria-Adriana Neag |
| collection | DOAJ |
| description | Non-steroidal anti-inflammatory drugs (NSAIDs) are some of the most widely used drugs due to their anti-inflammatory, analgesic and antipyretic pharmacological effects. Gastrointestinal side effects are some of the most severe and frequent side effects of NSAIDs. These depend on the balance of the gut microbiome, the abundance of Gram-negative bacteria, and the amount of lipopolysaccharide released. Therefore, restoring or improving gut bacteria balance with probiotic supplements could prove to be an adjuvant therapy against mild NSAID-induced enteropathy. Twenty-five Wistar albino male rats were divided into five groups. The negative control group was administered carboxymethylcellulose and the positive control group diclofenac (DIC), 8 mg/kg for 7 days, which represented the enteropathy model. Treatment groups consisted of a combination of pro-biotic spores (MSB), amino acids and immunoglobulins supplement (MM), which were also administered for 7 days. We analyzed hepatic injury markers (AST, ALT) and creatinine, and inflammatory markers, IL-6, TNF-α, PGE2, iNOS, as well as total antioxidant capacity. The results obtained in the present study suggest that the modulation of the intestinal microbiota by administration of probiotics (Bacillus spores), alone or in combination with immunoglobulins and amino acids, represents an attractive therapy for the prevention of NSAID-induced enteropathy. |
| first_indexed | 2024-03-09T20:39:12Z |
| format | Article |
| id | doaj.art-af1ea7b8025c426a8b20f783f1a2018e |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T20:39:12Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-af1ea7b8025c426a8b20f783f1a2018e2023-11-23T23:04:12ZengMDPI AGBiomedicines2227-90592022-10-011010250810.3390/biomedicines10102508The Role of Probiotic Bacillus Spores and Amino Acids with Immunoglobulins on a Rat Enteropathy ModelMaria-Adriana Neag0Carmen-Stanca Melincovici1Adrian Catinean2Dana-Maria Muntean3Raluca-Maria Pop4Ioana-Corina Bocsan5Andrei-Otto Mitre6Mihai-Bogdan Cardos7Andreea-Ioana Inceu8Anca-Dana Buzoianu9Department of Pharmacology, Toxicology and Clinical Pharmacology, Iuliu Hatieganu University of Medicine and Pharmacy, 400337 Cluj-Napoca, RomaniaHistology Discipline, Morphological Sciences Department, Iuliu Hatieganu University of Medicine and Pharmacy, 400349 Cluj-Napoca, RomaniaDepartment of Internal Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 400006 Cluj-Napoca, RomaniaDepartment of Pharmaceutical Technology and Biopharmaceutics, Iuliu Hatieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, RomaniaDepartment of Pharmacology, Toxicology and Clinical Pharmacology, Iuliu Hatieganu University of Medicine and Pharmacy, 400337 Cluj-Napoca, RomaniaDepartment of Pharmacology, Toxicology and Clinical Pharmacology, Iuliu Hatieganu University of Medicine and Pharmacy, 400337 Cluj-Napoca, RomaniaFaculty of Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 400349 Cluj-Napoca, RomaniaEmergency Clinical County Hospital Cluj-Napoca, 400006 Cluj-Napoca, RomaniaDepartment of Pharmacology, Toxicology and Clinical Pharmacology, Iuliu Hatieganu University of Medicine and Pharmacy, 400337 Cluj-Napoca, RomaniaDepartment of Pharmacology, Toxicology and Clinical Pharmacology, Iuliu Hatieganu University of Medicine and Pharmacy, 400337 Cluj-Napoca, RomaniaNon-steroidal anti-inflammatory drugs (NSAIDs) are some of the most widely used drugs due to their anti-inflammatory, analgesic and antipyretic pharmacological effects. Gastrointestinal side effects are some of the most severe and frequent side effects of NSAIDs. These depend on the balance of the gut microbiome, the abundance of Gram-negative bacteria, and the amount of lipopolysaccharide released. Therefore, restoring or improving gut bacteria balance with probiotic supplements could prove to be an adjuvant therapy against mild NSAID-induced enteropathy. Twenty-five Wistar albino male rats were divided into five groups. The negative control group was administered carboxymethylcellulose and the positive control group diclofenac (DIC), 8 mg/kg for 7 days, which represented the enteropathy model. Treatment groups consisted of a combination of pro-biotic spores (MSB), amino acids and immunoglobulins supplement (MM), which were also administered for 7 days. We analyzed hepatic injury markers (AST, ALT) and creatinine, and inflammatory markers, IL-6, TNF-α, PGE2, iNOS, as well as total antioxidant capacity. The results obtained in the present study suggest that the modulation of the intestinal microbiota by administration of probiotics (Bacillus spores), alone or in combination with immunoglobulins and amino acids, represents an attractive therapy for the prevention of NSAID-induced enteropathy.https://www.mdpi.com/2227-9059/10/10/2508NSAIDsprobioticsenteropathyinflammationmicrobiota |
| spellingShingle | Maria-Adriana Neag Carmen-Stanca Melincovici Adrian Catinean Dana-Maria Muntean Raluca-Maria Pop Ioana-Corina Bocsan Andrei-Otto Mitre Mihai-Bogdan Cardos Andreea-Ioana Inceu Anca-Dana Buzoianu The Role of Probiotic Bacillus Spores and Amino Acids with Immunoglobulins on a Rat Enteropathy Model Biomedicines NSAIDs probiotics enteropathy inflammation microbiota |
| title | The Role of Probiotic Bacillus Spores and Amino Acids with Immunoglobulins on a Rat Enteropathy Model |
| title_full | The Role of Probiotic Bacillus Spores and Amino Acids with Immunoglobulins on a Rat Enteropathy Model |
| title_fullStr | The Role of Probiotic Bacillus Spores and Amino Acids with Immunoglobulins on a Rat Enteropathy Model |
| title_full_unstemmed | The Role of Probiotic Bacillus Spores and Amino Acids with Immunoglobulins on a Rat Enteropathy Model |
| title_short | The Role of Probiotic Bacillus Spores and Amino Acids with Immunoglobulins on a Rat Enteropathy Model |
| title_sort | role of probiotic bacillus spores and amino acids with immunoglobulins on a rat enteropathy model |
| topic | NSAIDs probiotics enteropathy inflammation microbiota |
| url | https://www.mdpi.com/2227-9059/10/10/2508 |
| work_keys_str_mv | AT mariaadriananeag theroleofprobioticbacillussporesandaminoacidswithimmunoglobulinsonaratenteropathymodel AT carmenstancamelincovici theroleofprobioticbacillussporesandaminoacidswithimmunoglobulinsonaratenteropathymodel AT adriancatinean theroleofprobioticbacillussporesandaminoacidswithimmunoglobulinsonaratenteropathymodel AT danamariamuntean theroleofprobioticbacillussporesandaminoacidswithimmunoglobulinsonaratenteropathymodel AT ralucamariapop theroleofprobioticbacillussporesandaminoacidswithimmunoglobulinsonaratenteropathymodel AT ioanacorinabocsan theroleofprobioticbacillussporesandaminoacidswithimmunoglobulinsonaratenteropathymodel AT andreiottomitre theroleofprobioticbacillussporesandaminoacidswithimmunoglobulinsonaratenteropathymodel AT mihaibogdancardos theroleofprobioticbacillussporesandaminoacidswithimmunoglobulinsonaratenteropathymodel AT andreeaioanainceu theroleofprobioticbacillussporesandaminoacidswithimmunoglobulinsonaratenteropathymodel AT ancadanabuzoianu theroleofprobioticbacillussporesandaminoacidswithimmunoglobulinsonaratenteropathymodel AT mariaadriananeag roleofprobioticbacillussporesandaminoacidswithimmunoglobulinsonaratenteropathymodel AT carmenstancamelincovici roleofprobioticbacillussporesandaminoacidswithimmunoglobulinsonaratenteropathymodel AT adriancatinean roleofprobioticbacillussporesandaminoacidswithimmunoglobulinsonaratenteropathymodel AT danamariamuntean roleofprobioticbacillussporesandaminoacidswithimmunoglobulinsonaratenteropathymodel AT ralucamariapop roleofprobioticbacillussporesandaminoacidswithimmunoglobulinsonaratenteropathymodel AT ioanacorinabocsan roleofprobioticbacillussporesandaminoacidswithimmunoglobulinsonaratenteropathymodel AT andreiottomitre roleofprobioticbacillussporesandaminoacidswithimmunoglobulinsonaratenteropathymodel AT mihaibogdancardos roleofprobioticbacillussporesandaminoacidswithimmunoglobulinsonaratenteropathymodel AT andreeaioanainceu roleofprobioticbacillussporesandaminoacidswithimmunoglobulinsonaratenteropathymodel AT ancadanabuzoianu roleofprobioticbacillussporesandaminoacidswithimmunoglobulinsonaratenteropathymodel |