Bioinspired Synthesis of Magnetic Nanoparticles Based on Iron Oxides Using Orange Waste and Their Application as Photo-Activated Antibacterial Agents
Magnetic nanoparticles based on iron oxides (MNPs-Fe) have been proposed as photothermal agents (PTAs) within antibacterial photothermal therapy (PTT), aiming to counteract the vast health problem of multidrug-resistant bacterial infections. We present a quick and easy green synthesis (GS) to prepar...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/5/4770 |
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| author | David Giancarlo García Cristina Garzón-Romero Mateo Alejandro Salazar Karina J. Lagos Kleber Orlando Campaña Alexis Debut Karla Vizuete Miryan Rosita Rivera Dario Niebieskikwiat Maria J. Benitez María Paulina Romero |
| author_facet | David Giancarlo García Cristina Garzón-Romero Mateo Alejandro Salazar Karina J. Lagos Kleber Orlando Campaña Alexis Debut Karla Vizuete Miryan Rosita Rivera Dario Niebieskikwiat Maria J. Benitez María Paulina Romero |
| author_sort | David Giancarlo García |
| collection | DOAJ |
| description | Magnetic nanoparticles based on iron oxides (MNPs-Fe) have been proposed as photothermal agents (PTAs) within antibacterial photothermal therapy (PTT), aiming to counteract the vast health problem of multidrug-resistant bacterial infections. We present a quick and easy green synthesis (GS) to prepare MNPs-Fe harnessing waste. Orange peel extract (organic compounds) was used as a reducing, capping, and stabilizing agent in the GS, which employed microwave (MW) irradiation to reduce the synthesis time. The produced weight, physical–chemical features and magnetic features of the MNPs-Fe were studied. Moreover, their cytotoxicity was assessed in animal cell line ATCC RAW 264.7, as well as their antibacterial activity against <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>. We found that the 50GS-MNPs-Fe sample (prepared by GS, with 50% v/v of NH<sub>4</sub>OH and 50% v/v of orange peel extract) had an excellent mass yield. Its particle size was ~50 nm with the presence of an organic coating (terpenes or aldehydes). We believe that this coating improved the cell viability in extended periods (8 days) of cell culture with concentrations lower than 250 µg·mL<sup>−1</sup>, with respect to the MNPs-Fe obtained by CO and single MW, but it did not influence the antibacterial effect. The bacteria inhibition was attributed to the plasmonic of 50GS-MNPs-Fe (photothermal effect) by irradiation with red light (630 nm, 65.5 mW·cm<sup>−2</sup>, 30 min). We highlight the superparamagnetism of the 50GS-MNPs-Fe over 60 K in a broader temperature range than the MNPs-Fe obtained by CO (160.09 K) and MW (211.1 K). Therefore, 50GS-MNPs-Fe could be excellent candidates as broad-spectrum PTAs in antibacterial PTT. Furthermore, they might be employed in magnetic hyperthermia, magnetic resonance imaging, oncological treatments, and so on. |
| first_indexed | 2024-03-11T07:22:51Z |
| format | Article |
| id | doaj.art-af1f5e8f6dc1442db023c8b0c1b3c332 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T07:22:51Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-af1f5e8f6dc1442db023c8b0c1b3c3322023-11-17T07:53:08ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01245477010.3390/ijms24054770Bioinspired Synthesis of Magnetic Nanoparticles Based on Iron Oxides Using Orange Waste and Their Application as Photo-Activated Antibacterial AgentsDavid Giancarlo García0Cristina Garzón-Romero1Mateo Alejandro Salazar2Karina J. Lagos3Kleber Orlando Campaña4Alexis Debut5Karla Vizuete6Miryan Rosita Rivera7Dario Niebieskikwiat8Maria J. Benitez9María Paulina Romero10Department of Materials, Escuela Politécnica Nacional (EPN), Quito 170525, EcuadorLaboratorio de Investigación en Citogenética y Biomoléculas de Anfibios (LICBA), Centro de Investigación para la Salud en América Latina (CISeAL), Facultad de Ciencias Exactas y Naturales, Pontificia Universidad Católica del Ecuador (PUCE), Quito 170143, EcuadorLaboratorio de Investigación en Citogenética y Biomoléculas de Anfibios (LICBA), Centro de Investigación para la Salud en América Latina (CISeAL), Facultad de Ciencias Exactas y Naturales, Pontificia Universidad Católica del Ecuador (PUCE), Quito 170143, EcuadorDepartment of Materials, Escuela Politécnica Nacional (EPN), Quito 170525, EcuadorDepartment of Materials, Escuela Politécnica Nacional (EPN), Quito 170525, EcuadorCentro de Nanociencia y Nanotecnología, Universidad de Las Fuerzas Armadas ESPE, Sangolquí 171103, EcuadorCentro de Nanociencia y Nanotecnología, Universidad de Las Fuerzas Armadas ESPE, Sangolquí 171103, EcuadorLaboratorio de Investigación en Citogenética y Biomoléculas de Anfibios (LICBA), Centro de Investigación para la Salud en América Latina (CISeAL), Facultad de Ciencias Exactas y Naturales, Pontificia Universidad Católica del Ecuador (PUCE), Quito 170143, EcuadorDepartamento de Física, Colegio de Ciencias e Ingenierías, Universidad San Francisco de Quito, Quito 170901, EcuadorDepartamento de Física, Escuela Politécnica Nacional (EPN), Quito 170525, EcuadorDepartment of Materials, Escuela Politécnica Nacional (EPN), Quito 170525, EcuadorMagnetic nanoparticles based on iron oxides (MNPs-Fe) have been proposed as photothermal agents (PTAs) within antibacterial photothermal therapy (PTT), aiming to counteract the vast health problem of multidrug-resistant bacterial infections. We present a quick and easy green synthesis (GS) to prepare MNPs-Fe harnessing waste. Orange peel extract (organic compounds) was used as a reducing, capping, and stabilizing agent in the GS, which employed microwave (MW) irradiation to reduce the synthesis time. The produced weight, physical–chemical features and magnetic features of the MNPs-Fe were studied. Moreover, their cytotoxicity was assessed in animal cell line ATCC RAW 264.7, as well as their antibacterial activity against <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>. We found that the 50GS-MNPs-Fe sample (prepared by GS, with 50% v/v of NH<sub>4</sub>OH and 50% v/v of orange peel extract) had an excellent mass yield. Its particle size was ~50 nm with the presence of an organic coating (terpenes or aldehydes). We believe that this coating improved the cell viability in extended periods (8 days) of cell culture with concentrations lower than 250 µg·mL<sup>−1</sup>, with respect to the MNPs-Fe obtained by CO and single MW, but it did not influence the antibacterial effect. The bacteria inhibition was attributed to the plasmonic of 50GS-MNPs-Fe (photothermal effect) by irradiation with red light (630 nm, 65.5 mW·cm<sup>−2</sup>, 30 min). We highlight the superparamagnetism of the 50GS-MNPs-Fe over 60 K in a broader temperature range than the MNPs-Fe obtained by CO (160.09 K) and MW (211.1 K). Therefore, 50GS-MNPs-Fe could be excellent candidates as broad-spectrum PTAs in antibacterial PTT. Furthermore, they might be employed in magnetic hyperthermia, magnetic resonance imaging, oncological treatments, and so on.https://www.mdpi.com/1422-0067/24/5/4770green synthesisantibacterial PTTiron oxide nanoparticlesorange peel extractcytotoxicitysuperparamagnetic behavior |
| spellingShingle | David Giancarlo García Cristina Garzón-Romero Mateo Alejandro Salazar Karina J. Lagos Kleber Orlando Campaña Alexis Debut Karla Vizuete Miryan Rosita Rivera Dario Niebieskikwiat Maria J. Benitez María Paulina Romero Bioinspired Synthesis of Magnetic Nanoparticles Based on Iron Oxides Using Orange Waste and Their Application as Photo-Activated Antibacterial Agents International Journal of Molecular Sciences green synthesis antibacterial PTT iron oxide nanoparticles orange peel extract cytotoxicity superparamagnetic behavior |
| title | Bioinspired Synthesis of Magnetic Nanoparticles Based on Iron Oxides Using Orange Waste and Their Application as Photo-Activated Antibacterial Agents |
| title_full | Bioinspired Synthesis of Magnetic Nanoparticles Based on Iron Oxides Using Orange Waste and Their Application as Photo-Activated Antibacterial Agents |
| title_fullStr | Bioinspired Synthesis of Magnetic Nanoparticles Based on Iron Oxides Using Orange Waste and Their Application as Photo-Activated Antibacterial Agents |
| title_full_unstemmed | Bioinspired Synthesis of Magnetic Nanoparticles Based on Iron Oxides Using Orange Waste and Their Application as Photo-Activated Antibacterial Agents |
| title_short | Bioinspired Synthesis of Magnetic Nanoparticles Based on Iron Oxides Using Orange Waste and Their Application as Photo-Activated Antibacterial Agents |
| title_sort | bioinspired synthesis of magnetic nanoparticles based on iron oxides using orange waste and their application as photo activated antibacterial agents |
| topic | green synthesis antibacterial PTT iron oxide nanoparticles orange peel extract cytotoxicity superparamagnetic behavior |
| url | https://www.mdpi.com/1422-0067/24/5/4770 |
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