Study of T-cell immunoglobulin and mucin domain-3 expression profile in peripheral blood and bone marrow of human acute lymphoblastic leukemia patients
Background: Acute lymphoblastic leukemia (ALL) is a malignancy with aggressive tumors of immature lymphocytes. T-cell immunoglobulin and mucin-domain 3 (TIM-3) is a Type I transmembrane glycoprotein which is involved in cell proliferation. The objective of this research is to determine the TIM-3 exp...
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Wolters Kluwer Medknow Publications
2020-01-01
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Series: | Journal of Research in Medical Sciences |
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Online Access: | http://www.jmsjournal.net/article.asp?issn=1735-1995;year=2020;volume=25;issue=1;spage=69;epage=69;aulast=Balajam |
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author | Narges Zargar Balajam Mahdi Shabani Mahmoud Aghaei Mansoureh Haghighi Farzad Kompani |
author_facet | Narges Zargar Balajam Mahdi Shabani Mahmoud Aghaei Mansoureh Haghighi Farzad Kompani |
author_sort | Narges Zargar Balajam |
collection | DOAJ |
description | Background: Acute lymphoblastic leukemia (ALL) is a malignancy with aggressive tumors of immature lymphocytes. T-cell immunoglobulin and mucin-domain 3 (TIM-3) is a Type I transmembrane glycoprotein which is involved in cell proliferation. The objective of this research is to determine the TIM-3 expression in peripheral blood (PB) and bone marrow (BM) of 80 samples of normal and ALL patients. Materials and Methods: The amount of mRNA and protein of TIM-3 measured in the BM and PB the mononuclear layer of samples by real-time polymerase chain reaction and Western blotting. Results: Our findings indicated that relative mRNA expression of TIM-3 in PB and BM of the mononuclear layer of ALL patients was 1.7 and 5 times higher than normals, respectively. We also reported that the protein level of TIM-3 in mononuclear cells of ALL patients was 3.2-fold in BM and two-fold in PB more than normals. Conclusion: In conclusion, this study shows that TIM-3 increases in ALL patients, thus the expression of TIM-3 in tumor cells may be considered as a potential predictive factor in ALL patients, which needs to be explored in future. |
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format | Article |
id | doaj.art-af2176ef4bf9447ab5346d51d3f40b46 |
institution | Directory Open Access Journal |
issn | 1735-1995 1735-7136 |
language | English |
last_indexed | 2024-12-11T02:20:12Z |
publishDate | 2020-01-01 |
publisher | Wolters Kluwer Medknow Publications |
record_format | Article |
series | Journal of Research in Medical Sciences |
spelling | doaj.art-af2176ef4bf9447ab5346d51d3f40b462022-12-22T01:24:05ZengWolters Kluwer Medknow PublicationsJournal of Research in Medical Sciences1735-19951735-71362020-01-01251696910.4103/jrms.JRMS_759_19Study of T-cell immunoglobulin and mucin domain-3 expression profile in peripheral blood and bone marrow of human acute lymphoblastic leukemia patientsNarges Zargar BalajamMahdi ShabaniMahmoud AghaeiMansoureh HaghighiFarzad KompaniBackground: Acute lymphoblastic leukemia (ALL) is a malignancy with aggressive tumors of immature lymphocytes. T-cell immunoglobulin and mucin-domain 3 (TIM-3) is a Type I transmembrane glycoprotein which is involved in cell proliferation. The objective of this research is to determine the TIM-3 expression in peripheral blood (PB) and bone marrow (BM) of 80 samples of normal and ALL patients. Materials and Methods: The amount of mRNA and protein of TIM-3 measured in the BM and PB the mononuclear layer of samples by real-time polymerase chain reaction and Western blotting. Results: Our findings indicated that relative mRNA expression of TIM-3 in PB and BM of the mononuclear layer of ALL patients was 1.7 and 5 times higher than normals, respectively. We also reported that the protein level of TIM-3 in mononuclear cells of ALL patients was 3.2-fold in BM and two-fold in PB more than normals. Conclusion: In conclusion, this study shows that TIM-3 increases in ALL patients, thus the expression of TIM-3 in tumor cells may be considered as a potential predictive factor in ALL patients, which needs to be explored in future.http://www.jmsjournal.net/article.asp?issn=1735-1995;year=2020;volume=25;issue=1;spage=69;epage=69;aulast=Balajamgene expression profilingprecursor cell lymphoblastic leukemia-lymphomareal-time polymerase chain reactiont-cell immunoglobulin and mucin-domain 3 |
spellingShingle | Narges Zargar Balajam Mahdi Shabani Mahmoud Aghaei Mansoureh Haghighi Farzad Kompani Study of T-cell immunoglobulin and mucin domain-3 expression profile in peripheral blood and bone marrow of human acute lymphoblastic leukemia patients Journal of Research in Medical Sciences gene expression profiling precursor cell lymphoblastic leukemia-lymphoma real-time polymerase chain reaction t-cell immunoglobulin and mucin-domain 3 |
title | Study of T-cell immunoglobulin and mucin domain-3 expression profile in peripheral blood and bone marrow of human acute lymphoblastic leukemia patients |
title_full | Study of T-cell immunoglobulin and mucin domain-3 expression profile in peripheral blood and bone marrow of human acute lymphoblastic leukemia patients |
title_fullStr | Study of T-cell immunoglobulin and mucin domain-3 expression profile in peripheral blood and bone marrow of human acute lymphoblastic leukemia patients |
title_full_unstemmed | Study of T-cell immunoglobulin and mucin domain-3 expression profile in peripheral blood and bone marrow of human acute lymphoblastic leukemia patients |
title_short | Study of T-cell immunoglobulin and mucin domain-3 expression profile in peripheral blood and bone marrow of human acute lymphoblastic leukemia patients |
title_sort | study of t cell immunoglobulin and mucin domain 3 expression profile in peripheral blood and bone marrow of human acute lymphoblastic leukemia patients |
topic | gene expression profiling precursor cell lymphoblastic leukemia-lymphoma real-time polymerase chain reaction t-cell immunoglobulin and mucin-domain 3 |
url | http://www.jmsjournal.net/article.asp?issn=1735-1995;year=2020;volume=25;issue=1;spage=69;epage=69;aulast=Balajam |
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