Study of T-cell immunoglobulin and mucin domain-3 expression profile in peripheral blood and bone marrow of human acute lymphoblastic leukemia patients

Background: Acute lymphoblastic leukemia (ALL) is a malignancy with aggressive tumors of immature lymphocytes. T-cell immunoglobulin and mucin-domain 3 (TIM-3) is a Type I transmembrane glycoprotein which is involved in cell proliferation. The objective of this research is to determine the TIM-3 exp...

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Main Authors: Narges Zargar Balajam, Mahdi Shabani, Mahmoud Aghaei, Mansoureh Haghighi, Farzad Kompani
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2020-01-01
Series:Journal of Research in Medical Sciences
Subjects:
Online Access:http://www.jmsjournal.net/article.asp?issn=1735-1995;year=2020;volume=25;issue=1;spage=69;epage=69;aulast=Balajam
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author Narges Zargar Balajam
Mahdi Shabani
Mahmoud Aghaei
Mansoureh Haghighi
Farzad Kompani
author_facet Narges Zargar Balajam
Mahdi Shabani
Mahmoud Aghaei
Mansoureh Haghighi
Farzad Kompani
author_sort Narges Zargar Balajam
collection DOAJ
description Background: Acute lymphoblastic leukemia (ALL) is a malignancy with aggressive tumors of immature lymphocytes. T-cell immunoglobulin and mucin-domain 3 (TIM-3) is a Type I transmembrane glycoprotein which is involved in cell proliferation. The objective of this research is to determine the TIM-3 expression in peripheral blood (PB) and bone marrow (BM) of 80 samples of normal and ALL patients. Materials and Methods: The amount of mRNA and protein of TIM-3 measured in the BM and PB the mononuclear layer of samples by real-time polymerase chain reaction and Western blotting. Results: Our findings indicated that relative mRNA expression of TIM-3 in PB and BM of the mononuclear layer of ALL patients was 1.7 and 5 times higher than normals, respectively. We also reported that the protein level of TIM-3 in mononuclear cells of ALL patients was 3.2-fold in BM and two-fold in PB more than normals. Conclusion: In conclusion, this study shows that TIM-3 increases in ALL patients, thus the expression of TIM-3 in tumor cells may be considered as a potential predictive factor in ALL patients, which needs to be explored in future.
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spelling doaj.art-af2176ef4bf9447ab5346d51d3f40b462022-12-22T01:24:05ZengWolters Kluwer Medknow PublicationsJournal of Research in Medical Sciences1735-19951735-71362020-01-01251696910.4103/jrms.JRMS_759_19Study of T-cell immunoglobulin and mucin domain-3 expression profile in peripheral blood and bone marrow of human acute lymphoblastic leukemia patientsNarges Zargar BalajamMahdi ShabaniMahmoud AghaeiMansoureh HaghighiFarzad KompaniBackground: Acute lymphoblastic leukemia (ALL) is a malignancy with aggressive tumors of immature lymphocytes. T-cell immunoglobulin and mucin-domain 3 (TIM-3) is a Type I transmembrane glycoprotein which is involved in cell proliferation. The objective of this research is to determine the TIM-3 expression in peripheral blood (PB) and bone marrow (BM) of 80 samples of normal and ALL patients. Materials and Methods: The amount of mRNA and protein of TIM-3 measured in the BM and PB the mononuclear layer of samples by real-time polymerase chain reaction and Western blotting. Results: Our findings indicated that relative mRNA expression of TIM-3 in PB and BM of the mononuclear layer of ALL patients was 1.7 and 5 times higher than normals, respectively. We also reported that the protein level of TIM-3 in mononuclear cells of ALL patients was 3.2-fold in BM and two-fold in PB more than normals. Conclusion: In conclusion, this study shows that TIM-3 increases in ALL patients, thus the expression of TIM-3 in tumor cells may be considered as a potential predictive factor in ALL patients, which needs to be explored in future.http://www.jmsjournal.net/article.asp?issn=1735-1995;year=2020;volume=25;issue=1;spage=69;epage=69;aulast=Balajamgene expression profilingprecursor cell lymphoblastic leukemia-lymphomareal-time polymerase chain reactiont-cell immunoglobulin and mucin-domain 3
spellingShingle Narges Zargar Balajam
Mahdi Shabani
Mahmoud Aghaei
Mansoureh Haghighi
Farzad Kompani
Study of T-cell immunoglobulin and mucin domain-3 expression profile in peripheral blood and bone marrow of human acute lymphoblastic leukemia patients
Journal of Research in Medical Sciences
gene expression profiling
precursor cell lymphoblastic leukemia-lymphoma
real-time polymerase chain reaction
t-cell immunoglobulin and mucin-domain 3
title Study of T-cell immunoglobulin and mucin domain-3 expression profile in peripheral blood and bone marrow of human acute lymphoblastic leukemia patients
title_full Study of T-cell immunoglobulin and mucin domain-3 expression profile in peripheral blood and bone marrow of human acute lymphoblastic leukemia patients
title_fullStr Study of T-cell immunoglobulin and mucin domain-3 expression profile in peripheral blood and bone marrow of human acute lymphoblastic leukemia patients
title_full_unstemmed Study of T-cell immunoglobulin and mucin domain-3 expression profile in peripheral blood and bone marrow of human acute lymphoblastic leukemia patients
title_short Study of T-cell immunoglobulin and mucin domain-3 expression profile in peripheral blood and bone marrow of human acute lymphoblastic leukemia patients
title_sort study of t cell immunoglobulin and mucin domain 3 expression profile in peripheral blood and bone marrow of human acute lymphoblastic leukemia patients
topic gene expression profiling
precursor cell lymphoblastic leukemia-lymphoma
real-time polymerase chain reaction
t-cell immunoglobulin and mucin-domain 3
url http://www.jmsjournal.net/article.asp?issn=1735-1995;year=2020;volume=25;issue=1;spage=69;epage=69;aulast=Balajam
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