Genome-wide identification and characterization of the KCS gene family in sorghum (Sorghum bicolor (L.) Moench)
The aboveground parts of plants are covered with cuticle, a hydrophobic layer composed of cutin polyester and cuticular wax that can protect plants from various environmental stresses. β-Ketoacyl-CoA synthase (KCS) is the key rate-limiting enzyme in plant wax synthesis. Although the properties of KC...
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PeerJ Inc.
2022-10-01
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| Online Access: | https://peerj.com/articles/14156.pdf |
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| author | Aixia Zhang Jingjing Xu Xin Xu Junping Wu Ping Li Baohua Wang Hui Fang |
| author_facet | Aixia Zhang Jingjing Xu Xin Xu Junping Wu Ping Li Baohua Wang Hui Fang |
| author_sort | Aixia Zhang |
| collection | DOAJ |
| description | The aboveground parts of plants are covered with cuticle, a hydrophobic layer composed of cutin polyester and cuticular wax that can protect plants from various environmental stresses. β-Ketoacyl-CoA synthase (KCS) is the key rate-limiting enzyme in plant wax synthesis. Although the properties of KCS family genes have been investigated in many plant species, the understanding of this gene family in sorghum is still limited. Here, a total of 25 SbKCS genes were identified in the sorghum genome, which were named from SbKCS1 to SbKCS25. Evolutionary analysis among different species divided the KCS family into five subfamilies and the SbKCSs were more closely related to maize, implying a closer evolutionary relationship between sorghum and maize. All SbKCS genes were located on chromosomes 1, 2, 3, 4, 5, 6, 9 and 10, respectively, while Chr 1 and Chr 10 contained more KCS genes than other chromosomes. The prediction results of subcellular localization showed that SbKCSs were mainly expressed in the plasma membrane and mitochondria. Gene structure analysis revealed that there was 0–1 intron in the sorghum KCS family and SbKCSs within the same subgroup were similar. Multiple cis-acting elements related to abiotic stress, light and hormone response were enriched in the promoters of SbKCS genes, which indicated the functional diversity among these genes. The three-dimensional structure analysis showed that a compact spherical space structure was formed by various secondary bonds to maintain the stability of SbKCS proteins, which was necessary for their biological activity. qRT-PCR results revealed that nine randomly selected SbKCS genes expressed differently under drought and salt treatments, among which SbKCS8 showed the greatest fold of expression difference at 12 h after drought and salt stresses, which suggested that the SbKCS genes played a potential role in abiotic stress responses. Taken together, these results provided an insight into investigating the functions of KCS family in sorghum and in response to abiotic stress. |
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| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:27:26Z |
| publishDate | 2022-10-01 |
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| spelling | doaj.art-af21a8c2d54a4069b3e002d7c3b260942023-12-03T06:51:13ZengPeerJ Inc.PeerJ2167-83592022-10-0110e1415610.7717/peerj.14156Genome-wide identification and characterization of the KCS gene family in sorghum (Sorghum bicolor (L.) Moench)Aixia Zhang0Jingjing Xu1Xin Xu2Junping Wu3Ping Li4Baohua Wang5Hui Fang6Ministry of Agricultural Scientific Observing and Experimental Station of Maize in Plain Area of Southern Region, School of Life Sciences, Nantong University, Nantong, Jiangsu, ChinaMinistry of Agricultural Scientific Observing and Experimental Station of Maize in Plain Area of Southern Region, School of Life Sciences, Nantong University, Nantong, Jiangsu, ChinaMinistry of Agricultural Scientific Observing and Experimental Station of Maize in Plain Area of Southern Region, School of Life Sciences, Nantong University, Nantong, Jiangsu, ChinaNantong Changjiang Seed Co., Ltd, Nantong, Jiangsu, ChinaMinistry of Agricultural Scientific Observing and Experimental Station of Maize in Plain Area of Southern Region, School of Life Sciences, Nantong University, Nantong, Jiangsu, ChinaMinistry of Agricultural Scientific Observing and Experimental Station of Maize in Plain Area of Southern Region, School of Life Sciences, Nantong University, Nantong, Jiangsu, ChinaMinistry of Agricultural Scientific Observing and Experimental Station of Maize in Plain Area of Southern Region, School of Life Sciences, Nantong University, Nantong, Jiangsu, ChinaThe aboveground parts of plants are covered with cuticle, a hydrophobic layer composed of cutin polyester and cuticular wax that can protect plants from various environmental stresses. β-Ketoacyl-CoA synthase (KCS) is the key rate-limiting enzyme in plant wax synthesis. Although the properties of KCS family genes have been investigated in many plant species, the understanding of this gene family in sorghum is still limited. Here, a total of 25 SbKCS genes were identified in the sorghum genome, which were named from SbKCS1 to SbKCS25. Evolutionary analysis among different species divided the KCS family into five subfamilies and the SbKCSs were more closely related to maize, implying a closer evolutionary relationship between sorghum and maize. All SbKCS genes were located on chromosomes 1, 2, 3, 4, 5, 6, 9 and 10, respectively, while Chr 1 and Chr 10 contained more KCS genes than other chromosomes. The prediction results of subcellular localization showed that SbKCSs were mainly expressed in the plasma membrane and mitochondria. Gene structure analysis revealed that there was 0–1 intron in the sorghum KCS family and SbKCSs within the same subgroup were similar. Multiple cis-acting elements related to abiotic stress, light and hormone response were enriched in the promoters of SbKCS genes, which indicated the functional diversity among these genes. The three-dimensional structure analysis showed that a compact spherical space structure was formed by various secondary bonds to maintain the stability of SbKCS proteins, which was necessary for their biological activity. qRT-PCR results revealed that nine randomly selected SbKCS genes expressed differently under drought and salt treatments, among which SbKCS8 showed the greatest fold of expression difference at 12 h after drought and salt stresses, which suggested that the SbKCS genes played a potential role in abiotic stress responses. Taken together, these results provided an insight into investigating the functions of KCS family in sorghum and in response to abiotic stress.https://peerj.com/articles/14156.pdfSorghum bicolorKCS gene familyAbiotic stress |
| spellingShingle | Aixia Zhang Jingjing Xu Xin Xu Junping Wu Ping Li Baohua Wang Hui Fang Genome-wide identification and characterization of the KCS gene family in sorghum (Sorghum bicolor (L.) Moench) PeerJ Sorghum bicolor KCS gene family Abiotic stress |
| title | Genome-wide identification and characterization of the KCS gene family in sorghum (Sorghum bicolor (L.) Moench) |
| title_full | Genome-wide identification and characterization of the KCS gene family in sorghum (Sorghum bicolor (L.) Moench) |
| title_fullStr | Genome-wide identification and characterization of the KCS gene family in sorghum (Sorghum bicolor (L.) Moench) |
| title_full_unstemmed | Genome-wide identification and characterization of the KCS gene family in sorghum (Sorghum bicolor (L.) Moench) |
| title_short | Genome-wide identification and characterization of the KCS gene family in sorghum (Sorghum bicolor (L.) Moench) |
| title_sort | genome wide identification and characterization of the kcs gene family in sorghum sorghum bicolor l moench |
| topic | Sorghum bicolor KCS gene family Abiotic stress |
| url | https://peerj.com/articles/14156.pdf |
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