Extraction of Polysaccharides from Root of <i>Pseudostellaria heterophylla</i> (Miq.) Pax. and the Effects of Ultrasound Treatment on Its Properties and Antioxidant and Immune Activities
The hydrophilic polysaccharides (PS) were isolated and purified from the tuberous roots of <i>Pseudostellaria heterophylla</i>. The extraction process of PS from <i>Pesudostellariae radix</i> was optimized by single–factor experiments and orthogonal design. The extract was pu...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-12-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/29/1/142 |
| _version_ | 1797358412786302976 |
|---|---|
| author | Hangyu Li Ziwei Liu Qianqian Liu Xinnan Zhang Sheng Li Feng Tang Linzi Zhang Qian Yang Qiran Wang Shuyao Yang Ling Huang Yuwei Ba Xihui Du Falong Yang Haibo Feng |
| author_facet | Hangyu Li Ziwei Liu Qianqian Liu Xinnan Zhang Sheng Li Feng Tang Linzi Zhang Qian Yang Qiran Wang Shuyao Yang Ling Huang Yuwei Ba Xihui Du Falong Yang Haibo Feng |
| author_sort | Hangyu Li |
| collection | DOAJ |
| description | The hydrophilic polysaccharides (PS) were isolated and purified from the tuberous roots of <i>Pseudostellaria heterophylla</i>. The extraction process of PS from <i>Pesudostellariae radix</i> was optimized by single–factor experiments and orthogonal design. The extract was purified by DEAE cellulose column to obtain the pure polysaccharide PHP. Then PHP was treated with different intensities of sonication to study the effect of sonication on PHP’s characteristics and its biological activity in vitro and in vivo. The results of this study revealed that ultrasound treatment did not significantly change the properties of PHP. Further, with the increase of ultrasound intensity, PHP enhanced the proliferation and phagocytosis of macrophage RAW264.7. Meanwhile, it could also significantly improve the body’s antioxidant activity and immune function. The results of this study demonstrated that PHP has the potential as a food additive with enhanced antioxidant and immune functions, and its biological activities could be enhanced by sonication. |
| first_indexed | 2024-03-08T15:01:37Z |
| format | Article |
| id | doaj.art-af21cb4d41dc465a95f0130babb7de1c |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-08T15:01:37Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-af21cb4d41dc465a95f0130babb7de1c2024-01-10T15:04:17ZengMDPI AGMolecules1420-30492023-12-0129114210.3390/molecules29010142Extraction of Polysaccharides from Root of <i>Pseudostellaria heterophylla</i> (Miq.) Pax. and the Effects of Ultrasound Treatment on Its Properties and Antioxidant and Immune ActivitiesHangyu Li0Ziwei Liu1Qianqian Liu2Xinnan Zhang3Sheng Li4Feng Tang5Linzi Zhang6Qian Yang7Qiran Wang8Shuyao Yang9Ling Huang10Yuwei Ba11Xihui Du12Falong Yang13Haibo Feng14College of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, ChinaCollege of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, ChinaCollege of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, ChinaCollege of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, ChinaCollege of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, ChinaCollege of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, ChinaCollege of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, ChinaCollege of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, ChinaCollege of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, ChinaCollege of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, ChinaCollege of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, ChinaCollege of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, ChinaCollege of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, ChinaCollege of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, ChinaCollege of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, ChinaThe hydrophilic polysaccharides (PS) were isolated and purified from the tuberous roots of <i>Pseudostellaria heterophylla</i>. The extraction process of PS from <i>Pesudostellariae radix</i> was optimized by single–factor experiments and orthogonal design. The extract was purified by DEAE cellulose column to obtain the pure polysaccharide PHP. Then PHP was treated with different intensities of sonication to study the effect of sonication on PHP’s characteristics and its biological activity in vitro and in vivo. The results of this study revealed that ultrasound treatment did not significantly change the properties of PHP. Further, with the increase of ultrasound intensity, PHP enhanced the proliferation and phagocytosis of macrophage RAW264.7. Meanwhile, it could also significantly improve the body’s antioxidant activity and immune function. The results of this study demonstrated that PHP has the potential as a food additive with enhanced antioxidant and immune functions, and its biological activities could be enhanced by sonication.https://www.mdpi.com/1420-3049/29/1/142<i>Pesudostellariae radix</i>polysaccharidesultrasoundantioxidantimmune |
| spellingShingle | Hangyu Li Ziwei Liu Qianqian Liu Xinnan Zhang Sheng Li Feng Tang Linzi Zhang Qian Yang Qiran Wang Shuyao Yang Ling Huang Yuwei Ba Xihui Du Falong Yang Haibo Feng Extraction of Polysaccharides from Root of <i>Pseudostellaria heterophylla</i> (Miq.) Pax. and the Effects of Ultrasound Treatment on Its Properties and Antioxidant and Immune Activities Molecules <i>Pesudostellariae radix</i> polysaccharides ultrasound antioxidant immune |
| title | Extraction of Polysaccharides from Root of <i>Pseudostellaria heterophylla</i> (Miq.) Pax. and the Effects of Ultrasound Treatment on Its Properties and Antioxidant and Immune Activities |
| title_full | Extraction of Polysaccharides from Root of <i>Pseudostellaria heterophylla</i> (Miq.) Pax. and the Effects of Ultrasound Treatment on Its Properties and Antioxidant and Immune Activities |
| title_fullStr | Extraction of Polysaccharides from Root of <i>Pseudostellaria heterophylla</i> (Miq.) Pax. and the Effects of Ultrasound Treatment on Its Properties and Antioxidant and Immune Activities |
| title_full_unstemmed | Extraction of Polysaccharides from Root of <i>Pseudostellaria heterophylla</i> (Miq.) Pax. and the Effects of Ultrasound Treatment on Its Properties and Antioxidant and Immune Activities |
| title_short | Extraction of Polysaccharides from Root of <i>Pseudostellaria heterophylla</i> (Miq.) Pax. and the Effects of Ultrasound Treatment on Its Properties and Antioxidant and Immune Activities |
| title_sort | extraction of polysaccharides from root of i pseudostellaria heterophylla i miq pax and the effects of ultrasound treatment on its properties and antioxidant and immune activities |
| topic | <i>Pesudostellariae radix</i> polysaccharides ultrasound antioxidant immune |
| url | https://www.mdpi.com/1420-3049/29/1/142 |
| work_keys_str_mv | AT hangyuli extractionofpolysaccharidesfromrootofipseudostellariaheterophyllaimiqpaxandtheeffectsofultrasoundtreatmentonitspropertiesandantioxidantandimmuneactivities AT ziweiliu extractionofpolysaccharidesfromrootofipseudostellariaheterophyllaimiqpaxandtheeffectsofultrasoundtreatmentonitspropertiesandantioxidantandimmuneactivities AT qianqianliu extractionofpolysaccharidesfromrootofipseudostellariaheterophyllaimiqpaxandtheeffectsofultrasoundtreatmentonitspropertiesandantioxidantandimmuneactivities AT xinnanzhang extractionofpolysaccharidesfromrootofipseudostellariaheterophyllaimiqpaxandtheeffectsofultrasoundtreatmentonitspropertiesandantioxidantandimmuneactivities AT shengli extractionofpolysaccharidesfromrootofipseudostellariaheterophyllaimiqpaxandtheeffectsofultrasoundtreatmentonitspropertiesandantioxidantandimmuneactivities AT fengtang extractionofpolysaccharidesfromrootofipseudostellariaheterophyllaimiqpaxandtheeffectsofultrasoundtreatmentonitspropertiesandantioxidantandimmuneactivities AT linzizhang extractionofpolysaccharidesfromrootofipseudostellariaheterophyllaimiqpaxandtheeffectsofultrasoundtreatmentonitspropertiesandantioxidantandimmuneactivities AT qianyang extractionofpolysaccharidesfromrootofipseudostellariaheterophyllaimiqpaxandtheeffectsofultrasoundtreatmentonitspropertiesandantioxidantandimmuneactivities AT qiranwang extractionofpolysaccharidesfromrootofipseudostellariaheterophyllaimiqpaxandtheeffectsofultrasoundtreatmentonitspropertiesandantioxidantandimmuneactivities AT shuyaoyang extractionofpolysaccharidesfromrootofipseudostellariaheterophyllaimiqpaxandtheeffectsofultrasoundtreatmentonitspropertiesandantioxidantandimmuneactivities AT linghuang extractionofpolysaccharidesfromrootofipseudostellariaheterophyllaimiqpaxandtheeffectsofultrasoundtreatmentonitspropertiesandantioxidantandimmuneactivities AT yuweiba extractionofpolysaccharidesfromrootofipseudostellariaheterophyllaimiqpaxandtheeffectsofultrasoundtreatmentonitspropertiesandantioxidantandimmuneactivities AT xihuidu extractionofpolysaccharidesfromrootofipseudostellariaheterophyllaimiqpaxandtheeffectsofultrasoundtreatmentonitspropertiesandantioxidantandimmuneactivities AT falongyang extractionofpolysaccharidesfromrootofipseudostellariaheterophyllaimiqpaxandtheeffectsofultrasoundtreatmentonitspropertiesandantioxidantandimmuneactivities AT haibofeng extractionofpolysaccharidesfromrootofipseudostellariaheterophyllaimiqpaxandtheeffectsofultrasoundtreatmentonitspropertiesandantioxidantandimmuneactivities |