The Human Cytomegalovirus US31 Gene Predicts Favorable Survival and Regulates the Tumor Microenvironment in Gastric Cancer
Human cytomegalovirus (HCMV) is an oncogenic virus associated with tumorigenesis. Our previous study revealed that the HCMV US31 gene interacted with NF-κB2 and mediated inflammation through macrophages. However, there are few reports on the role of US31 in gastric cancer (GC). The aim of this study...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-04-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2021.614925/full |
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| author | Sisi Ye Sisi Ye Yuanbo Hu Yuanbo Hu Chenbin Chen Chenbin Chen Sian Chen Sian Chen Xinya Tong Huanbo Zhu Huanbo Zhu Bo Deng Xianjing Hu Xianjing Hu Xiangwei Sun Xiangwei Sun Xiaodong Chen Xiaodong Chen Xinyu Shi Ruihong Gu Wangkai Xie Wangkai Xie Gangqiang Guo Dong Xing Xian Shen Xiangyang Xue Xiangyang Xue Shurong Shen |
| author_facet | Sisi Ye Sisi Ye Yuanbo Hu Yuanbo Hu Chenbin Chen Chenbin Chen Sian Chen Sian Chen Xinya Tong Huanbo Zhu Huanbo Zhu Bo Deng Xianjing Hu Xianjing Hu Xiangwei Sun Xiangwei Sun Xiaodong Chen Xiaodong Chen Xinyu Shi Ruihong Gu Wangkai Xie Wangkai Xie Gangqiang Guo Dong Xing Xian Shen Xiangyang Xue Xiangyang Xue Shurong Shen |
| author_sort | Sisi Ye |
| collection | DOAJ |
| description | Human cytomegalovirus (HCMV) is an oncogenic virus associated with tumorigenesis. Our previous study revealed that the HCMV US31 gene interacted with NF-κB2 and mediated inflammation through macrophages. However, there are few reports on the role of US31 in gastric cancer (GC). The aim of this study was to investigate the expression of the US31 gene in GC tissue and assess its role in the occurrence and development of GC. US31 expression in 573 cancer tissues was analyzed using immunohistochemistry. Results showed that US31 was significantly associated with tumor size (P = 0.005) and distant metastasis (P < 0.001). Higher US31 expression indicated better overall survival in GC patients. Overexpression of US31 significantly inhibited the proliferation, migration, and invasion of GC cells in vitro (P < 0.05). Furthermore, expression levels of CD4, CD66b, and CD166 were positively correlated with US31, suggesting that it was involved in regulating the tumor immune microenvironment of GC. RNA sequencing, along with quantitative real-time polymerase chain reaction, confirmed that the expression of US31 promoted immune activation and secretion of inflammatory cytokines. Overall, US31 inhibited the malignant phenotype and regulated tumor immune cell infiltration in GC; these results suggest that US31 could be a potential prognostic factor for GC and may open the door for a new immunotherapy strategy. |
| first_indexed | 2024-12-16T16:02:16Z |
| format | Article |
| id | doaj.art-af24cc098df84291a5a1b6b95acb9e4a |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-16T16:02:16Z |
| publishDate | 2021-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-af24cc098df84291a5a1b6b95acb9e4a2022-12-21T22:25:26ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2021-04-011110.3389/fonc.2021.614925614925The Human Cytomegalovirus US31 Gene Predicts Favorable Survival and Regulates the Tumor Microenvironment in Gastric CancerSisi Ye0Sisi Ye1Yuanbo Hu2Yuanbo Hu3Chenbin Chen4Chenbin Chen5Sian Chen6Sian Chen7Xinya Tong8Huanbo Zhu9Huanbo Zhu10Bo Deng11Xianjing Hu12Xianjing Hu13Xiangwei Sun14Xiangwei Sun15Xiaodong Chen16Xiaodong Chen17Xinyu Shi18Ruihong Gu19Wangkai Xie20Wangkai Xie21Gangqiang Guo22Dong Xing23Xian Shen24Xiangyang Xue25Xiangyang Xue26Shurong Shen27Department of Medical Microbiology and Immunology, Wenzhou Medical University, Wenzhou, ChinaDepartment of Precision Medical Center Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Medical Microbiology and Immunology, Wenzhou Medical University, Wenzhou, ChinaDepartment of Gastrointestinal Surgery, Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Medical Microbiology and Immunology, Wenzhou Medical University, Wenzhou, ChinaDepartment of Gastrointestinal Surgery, Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Medical Microbiology and Immunology, Wenzhou Medical University, Wenzhou, ChinaDepartment of Gastrointestinal Surgery, Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Medical Microbiology and Immunology, Wenzhou Medical University, Wenzhou, ChinaDepartment of Medical Microbiology and Immunology, Wenzhou Medical University, Wenzhou, ChinaDepartment of Gastrointestinal Surgery, Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Medical Microbiology and Immunology, Wenzhou Medical University, Wenzhou, ChinaDepartment of Medical Microbiology and Immunology, Wenzhou Medical University, Wenzhou, ChinaDepartment of Gastrointestinal Surgery, Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Medical Microbiology and Immunology, Wenzhou Medical University, Wenzhou, ChinaDepartment of Gastrointestinal Surgery, Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Medical Microbiology and Immunology, Wenzhou Medical University, Wenzhou, ChinaDepartment of Gastrointestinal Surgery, Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Medical Microbiology and Immunology, Wenzhou Medical University, Wenzhou, ChinaDepartment of Medical Microbiology and Immunology, Wenzhou Medical University, Wenzhou, ChinaDepartment of Medical Microbiology and Immunology, Wenzhou Medical University, Wenzhou, ChinaDepartment of Gastrointestinal Surgery, Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Medical Microbiology and Immunology, Wenzhou Medical University, Wenzhou, ChinaDepartment of Medical Microbiology and Immunology, Wenzhou Medical University, Wenzhou, ChinaDepartment of Gastrointestinal Surgery, Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Medical Microbiology and Immunology, Wenzhou Medical University, Wenzhou, ChinaDepartment of Gastrointestinal Surgery, Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaWenzhou Hospital of Integrated Traditional Chinese and Western Medicine, Zhejiang Chinese Medical University, Wenzhou, ChinaHuman cytomegalovirus (HCMV) is an oncogenic virus associated with tumorigenesis. Our previous study revealed that the HCMV US31 gene interacted with NF-κB2 and mediated inflammation through macrophages. However, there are few reports on the role of US31 in gastric cancer (GC). The aim of this study was to investigate the expression of the US31 gene in GC tissue and assess its role in the occurrence and development of GC. US31 expression in 573 cancer tissues was analyzed using immunohistochemistry. Results showed that US31 was significantly associated with tumor size (P = 0.005) and distant metastasis (P < 0.001). Higher US31 expression indicated better overall survival in GC patients. Overexpression of US31 significantly inhibited the proliferation, migration, and invasion of GC cells in vitro (P < 0.05). Furthermore, expression levels of CD4, CD66b, and CD166 were positively correlated with US31, suggesting that it was involved in regulating the tumor immune microenvironment of GC. RNA sequencing, along with quantitative real-time polymerase chain reaction, confirmed that the expression of US31 promoted immune activation and secretion of inflammatory cytokines. Overall, US31 inhibited the malignant phenotype and regulated tumor immune cell infiltration in GC; these results suggest that US31 could be a potential prognostic factor for GC and may open the door for a new immunotherapy strategy.https://www.frontiersin.org/articles/10.3389/fonc.2021.614925/fullhuman cytomegalovirusgastric cancerUS31tumor immune microenvironmentprognostic factor |
| spellingShingle | Sisi Ye Sisi Ye Yuanbo Hu Yuanbo Hu Chenbin Chen Chenbin Chen Sian Chen Sian Chen Xinya Tong Huanbo Zhu Huanbo Zhu Bo Deng Xianjing Hu Xianjing Hu Xiangwei Sun Xiangwei Sun Xiaodong Chen Xiaodong Chen Xinyu Shi Ruihong Gu Wangkai Xie Wangkai Xie Gangqiang Guo Dong Xing Xian Shen Xiangyang Xue Xiangyang Xue Shurong Shen The Human Cytomegalovirus US31 Gene Predicts Favorable Survival and Regulates the Tumor Microenvironment in Gastric Cancer Frontiers in Oncology human cytomegalovirus gastric cancer US31 tumor immune microenvironment prognostic factor |
| title | The Human Cytomegalovirus US31 Gene Predicts Favorable Survival and Regulates the Tumor Microenvironment in Gastric Cancer |
| title_full | The Human Cytomegalovirus US31 Gene Predicts Favorable Survival and Regulates the Tumor Microenvironment in Gastric Cancer |
| title_fullStr | The Human Cytomegalovirus US31 Gene Predicts Favorable Survival and Regulates the Tumor Microenvironment in Gastric Cancer |
| title_full_unstemmed | The Human Cytomegalovirus US31 Gene Predicts Favorable Survival and Regulates the Tumor Microenvironment in Gastric Cancer |
| title_short | The Human Cytomegalovirus US31 Gene Predicts Favorable Survival and Regulates the Tumor Microenvironment in Gastric Cancer |
| title_sort | human cytomegalovirus us31 gene predicts favorable survival and regulates the tumor microenvironment in gastric cancer |
| topic | human cytomegalovirus gastric cancer US31 tumor immune microenvironment prognostic factor |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2021.614925/full |
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