An Efficient Bioemulsifier-Producing <em>Bacillus subtilis</em> UCP 0146 Isolated from Mangrove Sediments
In this work, we investigated the potential of <i>Bacillus subtilis</i> UCP 0146 in the bioconversion of a medium containing 100% cassava flour wastewater to obtain a bioemulsifier. The evaluation of the production was carried out by the emulsification index (IE<sub>24</sub>)...
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MDPI AG
2018-11-01
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| Series: | Colloids and Interfaces |
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| Online Access: | https://www.mdpi.com/2504-5377/2/4/58 |
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| author | Patrícia C. V. S. Maia Vanessa P. Santos Adriana S. Fereira Marcos A. C. Luna Thayse A. L. Silva Rosileide F. S. Andrade Galba M. Campos-Takaki |
| author_facet | Patrícia C. V. S. Maia Vanessa P. Santos Adriana S. Fereira Marcos A. C. Luna Thayse A. L. Silva Rosileide F. S. Andrade Galba M. Campos-Takaki |
| author_sort | Patrícia C. V. S. Maia |
| collection | DOAJ |
| description | In this work, we investigated the potential of <i>Bacillus subtilis</i> UCP 0146 in the bioconversion of a medium containing 100% cassava flour wastewater to obtain a bioemulsifier. The evaluation of the production was carried out by the emulsification index (IE<sub>24</sub>) and the surface tension (ST). The ionic charge, stability (temperature, salinity, and pH measured by IE<sub>24</sub> and viscosity), and ability to remove and disperse oil and textile dye were investigated. <i>B. subtilis</i> produced an anionic bioemulsifier in the medium containing 100% cassava wastewater under Condition 4 of the factorial design (inoculum 9% at a temperature of 35 °C and shaken at 100 rpm), and showed a surface tension of 39 mN/m, an IE<sub>24</sub> of 95.2%, and a yield of 2.69 g·L<sup>−1</sup>. The bioemulsifier showed stability at different pH (2–8), temperatures (0–120 °C), and NaCl concentrations, a dispersion oil displacement area (ODA) test of 55.83 cm<sup>2</sup>, and a reduction of the viscosity of the burned engine oil (90.5 Cp). The bioemulsifier was able to remove petroleum (94.4%) and methylene blue azo dye (62.2%). The bioemulsifier and its synthesis from bacteria also emphasizes the role of surfactants in oil remediation. |
| first_indexed | 2024-04-12T19:15:02Z |
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| issn | 2504-5377 |
| language | English |
| last_indexed | 2024-04-12T19:15:02Z |
| publishDate | 2018-11-01 |
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| record_format | Article |
| series | Colloids and Interfaces |
| spelling | doaj.art-af259207f503463fbaad3290b4003ffc2022-12-22T03:19:47ZengMDPI AGColloids and Interfaces2504-53772018-11-01245810.3390/colloids2040058colloids2040058An Efficient Bioemulsifier-Producing <em>Bacillus subtilis</em> UCP 0146 Isolated from Mangrove SedimentsPatrícia C. V. S. Maia0Vanessa P. Santos1Adriana S. Fereira2Marcos A. C. Luna3Thayse A. L. Silva4Rosileide F. S. Andrade5Galba M. Campos-Takaki6Development of Environmental Processes Post-Graduation Program, Catholic University of Pernambuco, Recife 50050-900, BrazilDevelopment of Environmental Processes Post-Graduation Program, Catholic University of Pernambuco, Recife 50050-900, BrazilDoctorate Northeast Network for Biotechnology, Federal Rural University of Pernambuco, Recife 52171-900, BrazilDoctorate Northeast Network for Biotechnology, Federal Rural University of Pernambuco, Recife 52171-900, BrazilNational Post-Doctorate Program-CAPES, Catholic University of Pernambuco, Recife 50050-900, BrazilNational Post-Doctorate Program-CAPES, Catholic University of Pernambuco, Recife 50050-900, BrazilNucleus of Research in Environmental Sciences and Biotechnology, Catholic University of Pernambuco, Recife 50050-590, BrazilIn this work, we investigated the potential of <i>Bacillus subtilis</i> UCP 0146 in the bioconversion of a medium containing 100% cassava flour wastewater to obtain a bioemulsifier. The evaluation of the production was carried out by the emulsification index (IE<sub>24</sub>) and the surface tension (ST). The ionic charge, stability (temperature, salinity, and pH measured by IE<sub>24</sub> and viscosity), and ability to remove and disperse oil and textile dye were investigated. <i>B. subtilis</i> produced an anionic bioemulsifier in the medium containing 100% cassava wastewater under Condition 4 of the factorial design (inoculum 9% at a temperature of 35 °C and shaken at 100 rpm), and showed a surface tension of 39 mN/m, an IE<sub>24</sub> of 95.2%, and a yield of 2.69 g·L<sup>−1</sup>. The bioemulsifier showed stability at different pH (2–8), temperatures (0–120 °C), and NaCl concentrations, a dispersion oil displacement area (ODA) test of 55.83 cm<sup>2</sup>, and a reduction of the viscosity of the burned engine oil (90.5 Cp). The bioemulsifier was able to remove petroleum (94.4%) and methylene blue azo dye (62.2%). The bioemulsifier and its synthesis from bacteria also emphasizes the role of surfactants in oil remediation.https://www.mdpi.com/2504-5377/2/4/58<i>Bacillus subtilis</i>bioemulsifiercassava wastewaterremoval pollutantmethylene blue dye |
| spellingShingle | Patrícia C. V. S. Maia Vanessa P. Santos Adriana S. Fereira Marcos A. C. Luna Thayse A. L. Silva Rosileide F. S. Andrade Galba M. Campos-Takaki An Efficient Bioemulsifier-Producing <em>Bacillus subtilis</em> UCP 0146 Isolated from Mangrove Sediments Colloids and Interfaces <i>Bacillus subtilis</i> bioemulsifier cassava wastewater removal pollutant methylene blue dye |
| title | An Efficient Bioemulsifier-Producing <em>Bacillus subtilis</em> UCP 0146 Isolated from Mangrove Sediments |
| title_full | An Efficient Bioemulsifier-Producing <em>Bacillus subtilis</em> UCP 0146 Isolated from Mangrove Sediments |
| title_fullStr | An Efficient Bioemulsifier-Producing <em>Bacillus subtilis</em> UCP 0146 Isolated from Mangrove Sediments |
| title_full_unstemmed | An Efficient Bioemulsifier-Producing <em>Bacillus subtilis</em> UCP 0146 Isolated from Mangrove Sediments |
| title_short | An Efficient Bioemulsifier-Producing <em>Bacillus subtilis</em> UCP 0146 Isolated from Mangrove Sediments |
| title_sort | efficient bioemulsifier producing em bacillus subtilis em ucp 0146 isolated from mangrove sediments |
| topic | <i>Bacillus subtilis</i> bioemulsifier cassava wastewater removal pollutant methylene blue dye |
| url | https://www.mdpi.com/2504-5377/2/4/58 |
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