Colorectal Adenocarcinoma Cell Culture in a Microfluidically Controlled Environment with a Static Molecular Gradient of Polyphenol
To study the simultaneous effect of the molecular gradient of polyphenols (curcumin, <i>trans</i>-resveratrol, and wogonin) and biological factors released from tumor cells on apoptosis of adjacent cells, a novel microfluidic system was designed and manufactured. The small height/volume...
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MDPI AG
2021-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/11/3215 |
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| author | Roman G. Szafran Kazimierz Gąsiorowski Benita Wiatrak |
| author_facet | Roman G. Szafran Kazimierz Gąsiorowski Benita Wiatrak |
| author_sort | Roman G. Szafran |
| collection | DOAJ |
| description | To study the simultaneous effect of the molecular gradient of polyphenols (curcumin, <i>trans</i>-resveratrol, and wogonin) and biological factors released from tumor cells on apoptosis of adjacent cells, a novel microfluidic system was designed and manufactured. The small height/volume of microfluidic culture chambers and static conditions allowed for establishing the local microenvironment and maintaining undisturbed concentration profiles of naturally secreted from cells biochemical factors. In all trials, we observe that these conditions significantly affect cell viability by stimulating cell apoptosis at lower concentrations of polyphenols than in traditional multiwell cultures. The observed difference varied between 20.4–87.8% for curcumin, 11.0–37.5% for resveratrol, and 21.7–62.2% for wogonin. At low concentrations of polyphenols, the proapoptotic substances released from adjacent cells, like protein degradation products, significantly influence cell viability. The mean increase in cell mortality was 38.3% for microfluidic cultures. Our research has also confirmed that the gradient microsystem is useful in routine laboratory tests in the same way as a multiwell plate and may be treated as its replacement in the future. We elaborated the new repetitive procedures for cell culture and tests in static gradient conditions, which may become a gold standard of new drug investigations in the future. |
| first_indexed | 2024-03-10T10:59:41Z |
| format | Article |
| id | doaj.art-af2ec98ab87945b0b7069fef04bc5d8a |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T10:59:41Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-af2ec98ab87945b0b7069fef04bc5d8a2023-11-21T21:38:54ZengMDPI AGMolecules1420-30492021-05-012611321510.3390/molecules26113215Colorectal Adenocarcinoma Cell Culture in a Microfluidically Controlled Environment with a Static Molecular Gradient of PolyphenolRoman G. Szafran0Kazimierz Gąsiorowski1Benita Wiatrak2Department of Biochemistry, Molecular Biology and Biotechnology, Faculty of Chemistry, Wroclaw University of Science and Technology, ul. Norwida 4/6, 50-373 Wroclaw, PolandDepartment of Basic Medical Sciences, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211, 50-556 Wroclaw, PolandDepartment of Basic Medical Sciences, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211, 50-556 Wroclaw, PolandTo study the simultaneous effect of the molecular gradient of polyphenols (curcumin, <i>trans</i>-resveratrol, and wogonin) and biological factors released from tumor cells on apoptosis of adjacent cells, a novel microfluidic system was designed and manufactured. The small height/volume of microfluidic culture chambers and static conditions allowed for establishing the local microenvironment and maintaining undisturbed concentration profiles of naturally secreted from cells biochemical factors. In all trials, we observe that these conditions significantly affect cell viability by stimulating cell apoptosis at lower concentrations of polyphenols than in traditional multiwell cultures. The observed difference varied between 20.4–87.8% for curcumin, 11.0–37.5% for resveratrol, and 21.7–62.2% for wogonin. At low concentrations of polyphenols, the proapoptotic substances released from adjacent cells, like protein degradation products, significantly influence cell viability. The mean increase in cell mortality was 38.3% for microfluidic cultures. Our research has also confirmed that the gradient microsystem is useful in routine laboratory tests in the same way as a multiwell plate and may be treated as its replacement in the future. We elaborated the new repetitive procedures for cell culture and tests in static gradient conditions, which may become a gold standard of new drug investigations in the future.https://www.mdpi.com/1420-3049/26/11/3215cancertumor-on-a-chipmicrofluidic devicecurcumin<i>trans</i>-resveratrolwogonin |
| spellingShingle | Roman G. Szafran Kazimierz Gąsiorowski Benita Wiatrak Colorectal Adenocarcinoma Cell Culture in a Microfluidically Controlled Environment with a Static Molecular Gradient of Polyphenol Molecules cancer tumor-on-a-chip microfluidic device curcumin <i>trans</i>-resveratrol wogonin |
| title | Colorectal Adenocarcinoma Cell Culture in a Microfluidically Controlled Environment with a Static Molecular Gradient of Polyphenol |
| title_full | Colorectal Adenocarcinoma Cell Culture in a Microfluidically Controlled Environment with a Static Molecular Gradient of Polyphenol |
| title_fullStr | Colorectal Adenocarcinoma Cell Culture in a Microfluidically Controlled Environment with a Static Molecular Gradient of Polyphenol |
| title_full_unstemmed | Colorectal Adenocarcinoma Cell Culture in a Microfluidically Controlled Environment with a Static Molecular Gradient of Polyphenol |
| title_short | Colorectal Adenocarcinoma Cell Culture in a Microfluidically Controlled Environment with a Static Molecular Gradient of Polyphenol |
| title_sort | colorectal adenocarcinoma cell culture in a microfluidically controlled environment with a static molecular gradient of polyphenol |
| topic | cancer tumor-on-a-chip microfluidic device curcumin <i>trans</i>-resveratrol wogonin |
| url | https://www.mdpi.com/1420-3049/26/11/3215 |
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