YOUNG AGE CHILDREN WITH INTESTINE DYSBIOSIS AS CARRIERS OF ENTEROAGGREGATIVE ESCHERICHIA COLI
Aim. Study the prevalence of diarrhea-genic E. coli of the enteroaggregative group in children with intestine dysbiosis. Materials and methods. PCR method was used to study virulence factors in 511 strains of E. coli isolated during bacteriologic study of feces samples from 393 children aged less th...
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| Format: | Article |
| Language: | Russian |
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Central Research Institute for Epidemiology
2017-08-01
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| Series: | Журнал микробиологии, эпидемиологии и иммунобиологии |
| Subjects: | |
| Online Access: | https://microbiol.elpub.ru/jour/article/view/179 |
| _version_ | 1811284739280076800 |
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| author | M. A. Makarova L. V. Suzhaeva L. A. Kaftyreva |
| author_facet | M. A. Makarova L. V. Suzhaeva L. A. Kaftyreva |
| author_sort | M. A. Makarova |
| collection | DOAJ |
| description | Aim. Study the prevalence of diarrhea-genic E. coli of the enteroaggregative group in children with intestine dysbiosis. Materials and methods. PCR method was used to study virulence factors in 511 strains of E. coli isolated during bacteriologic study of feces samples from 393 children aged less than 2 years. Sensitivity to antibiotics was determined by disc-diffusion method, results interpretation - according to clinical recommendations Determination of sensitivity of microorganisms to antimicrobial preparations, 2015. Results. 23 enteroaggregative E. coli strains were identified (EAggEC). All the strains had aaf gene coding aggregative-adhesion fimbriae and 4 other genes (aggR, ast, aap, aatA) in various combinations coding virulence factors EAggEC. 19 strains (87%) were not sensitive to antimicrobial preparations. Resistance to extended spectrum cephalosporins was determined by the production of extended spectrum beta-lactamase (ESBL) of CTX-M genetic family and AmpC cephalosporinase. Conclusion. Results of the study have shown that 6% of children with intestine dysbiosis are EAggEC carriers, that gives evidence on the necessity of detection of EAggEC strains - a novel group of diarrhea-genic E. coli not only in patients with diarrhea syndrome, but also using intestine dysbiosis. |
| first_indexed | 2024-04-13T02:33:31Z |
| format | Article |
| id | doaj.art-af310ab6f4b5416babf6ec1c13df0afa |
| institution | Directory Open Access Journal |
| issn | 0372-9311 2686-7613 |
| language | Russian |
| last_indexed | 2024-04-13T02:33:31Z |
| publishDate | 2017-08-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Журнал микробиологии, эпидемиологии и иммунобиологии |
| spelling | doaj.art-af310ab6f4b5416babf6ec1c13df0afa2022-12-22T03:06:28ZrusCentral Research Institute for EpidemiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132017-08-0104545810.36233/0372-9311-2017-4-54-58179YOUNG AGE CHILDREN WITH INTESTINE DYSBIOSIS AS CARRIERS OF ENTEROAGGREGATIVE ESCHERICHIA COLIM. A. Makarova0L. V. Suzhaeva1L. A. Kaftyreva2Pasteur St. Petersburg Research Institute of Epidemiology and MicrobiologyPasteur St. Petersburg Research Institute of Epidemiology and MicrobiologyPasteur St. Petersburg Research Institute of Epidemiology and MicrobiologyAim. Study the prevalence of diarrhea-genic E. coli of the enteroaggregative group in children with intestine dysbiosis. Materials and methods. PCR method was used to study virulence factors in 511 strains of E. coli isolated during bacteriologic study of feces samples from 393 children aged less than 2 years. Sensitivity to antibiotics was determined by disc-diffusion method, results interpretation - according to clinical recommendations Determination of sensitivity of microorganisms to antimicrobial preparations, 2015. Results. 23 enteroaggregative E. coli strains were identified (EAggEC). All the strains had aaf gene coding aggregative-adhesion fimbriae and 4 other genes (aggR, ast, aap, aatA) in various combinations coding virulence factors EAggEC. 19 strains (87%) were not sensitive to antimicrobial preparations. Resistance to extended spectrum cephalosporins was determined by the production of extended spectrum beta-lactamase (ESBL) of CTX-M genetic family and AmpC cephalosporinase. Conclusion. Results of the study have shown that 6% of children with intestine dysbiosis are EAggEC carriers, that gives evidence on the necessity of detection of EAggEC strains - a novel group of diarrhea-genic E. coli not only in patients with diarrhea syndrome, but also using intestine dysbiosis.https://microbiol.elpub.ru/jour/article/view/179entero-aggregative escherichia coli (eaggec)virulence factorsresistance to antimicrobial preparationschildren |
| spellingShingle | M. A. Makarova L. V. Suzhaeva L. A. Kaftyreva YOUNG AGE CHILDREN WITH INTESTINE DYSBIOSIS AS CARRIERS OF ENTEROAGGREGATIVE ESCHERICHIA COLI Журнал микробиологии, эпидемиологии и иммунобиологии entero-aggregative escherichia coli (eaggec) virulence factors resistance to antimicrobial preparations children |
| title | YOUNG AGE CHILDREN WITH INTESTINE DYSBIOSIS AS CARRIERS OF ENTEROAGGREGATIVE ESCHERICHIA COLI |
| title_full | YOUNG AGE CHILDREN WITH INTESTINE DYSBIOSIS AS CARRIERS OF ENTEROAGGREGATIVE ESCHERICHIA COLI |
| title_fullStr | YOUNG AGE CHILDREN WITH INTESTINE DYSBIOSIS AS CARRIERS OF ENTEROAGGREGATIVE ESCHERICHIA COLI |
| title_full_unstemmed | YOUNG AGE CHILDREN WITH INTESTINE DYSBIOSIS AS CARRIERS OF ENTEROAGGREGATIVE ESCHERICHIA COLI |
| title_short | YOUNG AGE CHILDREN WITH INTESTINE DYSBIOSIS AS CARRIERS OF ENTEROAGGREGATIVE ESCHERICHIA COLI |
| title_sort | young age children with intestine dysbiosis as carriers of enteroaggregative escherichia coli |
| topic | entero-aggregative escherichia coli (eaggec) virulence factors resistance to antimicrobial preparations children |
| url | https://microbiol.elpub.ru/jour/article/view/179 |
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