Inactivation of Salmonella using ultrasound in combination with Litsea cubeba essential oil nanoemulsion and its bactericidal application on cherry tomatoes
The presence of Salmonella in nature poses a significant and unacceptable threat to the human public health domain. In this study, the antibacterial effect and mechanism of ultrasound (US) combined with Litsea cubeba essential oil nanoemulsion (LEON) on Salmonella. LEON + US treatment has a signific...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-08-01
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| Series: | Ultrasonics Sonochemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1350417723001931 |
| _version_ | 1797754045375447040 |
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| author | Ruiying Su Xinyi Guo Shuai Cheng Ziruo Zhang Hui Yang Jingzi Wang Luyi Song Zhande Liu Yutang Wang Xin Lü Chao Shi |
| author_facet | Ruiying Su Xinyi Guo Shuai Cheng Ziruo Zhang Hui Yang Jingzi Wang Luyi Song Zhande Liu Yutang Wang Xin Lü Chao Shi |
| author_sort | Ruiying Su |
| collection | DOAJ |
| description | The presence of Salmonella in nature poses a significant and unacceptable threat to the human public health domain. In this study, the antibacterial effect and mechanism of ultrasound (US) combined with Litsea cubeba essential oil nanoemulsion (LEON) on Salmonella. LEON + US treatment has a significant bactericidal effect on Salmonella. Reactive oxygen species (ROS), malondialdehyde (MDA) detection, N-phenyl-l-naphthylamine (NPN) uptake and nucleic acid release assays showed that LEON + US exacerbated cell membrane lipid peroxidation and increased the permeability of the cell membrane. The results of field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) showed that LEON + US treatment was able to alter cell morphology. It can be observed by flow cytometry (FCM) that LEON + US treatment can cause cell apoptosis. In addition, bacterial counts of cherry tomatoes treated with LEON (0.08 μL/mL) + US (345 W/cm2) for 9 min were reduced by 6.50 ± 0.20 log CFU/mL. This study demonstrates that LEON + US treatment can be an effective way to improve the safety of fruits and vegetables in the food industry. |
| first_indexed | 2024-03-12T17:26:48Z |
| format | Article |
| id | doaj.art-af338c374845467e9e857f8c39aaa92f |
| institution | Directory Open Access Journal |
| issn | 1350-4177 |
| language | English |
| last_indexed | 2024-03-12T17:26:48Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ultrasonics Sonochemistry |
| spelling | doaj.art-af338c374845467e9e857f8c39aaa92f2023-08-05T05:15:23ZengElsevierUltrasonics Sonochemistry1350-41772023-08-0198106481Inactivation of Salmonella using ultrasound in combination with Litsea cubeba essential oil nanoemulsion and its bactericidal application on cherry tomatoesRuiying Su0Xinyi Guo1Shuai Cheng2Ziruo Zhang3Hui Yang4Jingzi Wang5Luyi Song6Zhande Liu7Yutang Wang8Xin Lü9Chao Shi10College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, ChinaSchool of Science, Xi’an Jiaotong-Liverpool University, Suzhou 215123, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, ChinaCollege of Horticulture, Northwest A＆F University, Yangling, Shaanxi, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China; Corresponding author at: College of Food Science and Engineering, Northwest A&F University, 22 Xinong Road, Yangling, Shaanxi 712100, China.The presence of Salmonella in nature poses a significant and unacceptable threat to the human public health domain. In this study, the antibacterial effect and mechanism of ultrasound (US) combined with Litsea cubeba essential oil nanoemulsion (LEON) on Salmonella. LEON + US treatment has a significant bactericidal effect on Salmonella. Reactive oxygen species (ROS), malondialdehyde (MDA) detection, N-phenyl-l-naphthylamine (NPN) uptake and nucleic acid release assays showed that LEON + US exacerbated cell membrane lipid peroxidation and increased the permeability of the cell membrane. The results of field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) showed that LEON + US treatment was able to alter cell morphology. It can be observed by flow cytometry (FCM) that LEON + US treatment can cause cell apoptosis. In addition, bacterial counts of cherry tomatoes treated with LEON (0.08 μL/mL) + US (345 W/cm2) for 9 min were reduced by 6.50 ± 0.20 log CFU/mL. This study demonstrates that LEON + US treatment can be an effective way to improve the safety of fruits and vegetables in the food industry.http://www.sciencedirect.com/science/article/pii/S1350417723001931SalmonellaLitsea cubeba essential oil nanoemulsionUltrasoundCombined bactericidal effectCherry tomatoes |
| spellingShingle | Ruiying Su Xinyi Guo Shuai Cheng Ziruo Zhang Hui Yang Jingzi Wang Luyi Song Zhande Liu Yutang Wang Xin Lü Chao Shi Inactivation of Salmonella using ultrasound in combination with Litsea cubeba essential oil nanoemulsion and its bactericidal application on cherry tomatoes Ultrasonics Sonochemistry Salmonella Litsea cubeba essential oil nanoemulsion Ultrasound Combined bactericidal effect Cherry tomatoes |
| title | Inactivation of Salmonella using ultrasound in combination with Litsea cubeba essential oil nanoemulsion and its bactericidal application on cherry tomatoes |
| title_full | Inactivation of Salmonella using ultrasound in combination with Litsea cubeba essential oil nanoemulsion and its bactericidal application on cherry tomatoes |
| title_fullStr | Inactivation of Salmonella using ultrasound in combination with Litsea cubeba essential oil nanoemulsion and its bactericidal application on cherry tomatoes |
| title_full_unstemmed | Inactivation of Salmonella using ultrasound in combination with Litsea cubeba essential oil nanoemulsion and its bactericidal application on cherry tomatoes |
| title_short | Inactivation of Salmonella using ultrasound in combination with Litsea cubeba essential oil nanoemulsion and its bactericidal application on cherry tomatoes |
| title_sort | inactivation of salmonella using ultrasound in combination with litsea cubeba essential oil nanoemulsion and its bactericidal application on cherry tomatoes |
| topic | Salmonella Litsea cubeba essential oil nanoemulsion Ultrasound Combined bactericidal effect Cherry tomatoes |
| url | http://www.sciencedirect.com/science/article/pii/S1350417723001931 |
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