Development of a system for testing apple resistance to Erwinia amylovora using in vitro culture techniques
The suitability of different in vitro artificial inoculation methods for testing apple resistance to fire blight was evaluated. Six proliferation MS media containing BAP, TDZ or 2iP were tested to determine favourable conditions for shoot development. Out of the tested cytokinins, only media with BA...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2015-03-01
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| Series: | Plant Protection Science |
| Subjects: | |
| Online Access: | https://pps.agriculturejournals.cz/artkey/pps-201501-0001_development-of-a-system-for-testing-apple-resistance-to-erwinia-amylovora-using-in-vitro-culture-techniques.php |
| _version_ | 1797898508070551552 |
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| author | Jiří SEDLÁK František PAPRŠTEIN Josef KORBA Jana ŠILLEROVÁ |
| author_facet | Jiří SEDLÁK František PAPRŠTEIN Josef KORBA Jana ŠILLEROVÁ |
| author_sort | Jiří SEDLÁK |
| collection | DOAJ |
| description | The suitability of different in vitro artificial inoculation methods for testing apple resistance to fire blight was evaluated. Six proliferation MS media containing BAP, TDZ or 2iP were tested to determine favourable conditions for shoot development. Out of the tested cytokinins, only media with BAP proved to be useful for all tested cultivars for artificial inoculation with Erwinia amylovora. Out of three tested inoculation methods, removal of 3 mm of the shoot apex with scissors and transfer of bacterial suspension by pipette (method 1) appeared to be the most suitable technique. This method enabled the determination of susceptibility of two cultivars due to the different speed of fire blight symptom development. |
| first_indexed | 2024-04-10T08:15:28Z |
| format | Article |
| id | doaj.art-af386ea1d33247ab8afcc2f0e5fdf6cb |
| institution | Directory Open Access Journal |
| issn | 1212-2580 1805-9341 |
| language | English |
| last_indexed | 2024-04-10T08:15:28Z |
| publishDate | 2015-03-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Plant Protection Science |
| spelling | doaj.art-af386ea1d33247ab8afcc2f0e5fdf6cb2023-02-23T03:44:14ZengCzech Academy of Agricultural SciencesPlant Protection Science1212-25801805-93412015-03-015111510.17221/94/2013-PPSpps-201501-0001Development of a system for testing apple resistance to Erwinia amylovora using in vitro culture techniquesJiří SEDLÁK0František PAPRŠTEIN1Josef KORBA2Jana ŠILLEROVÁ3Research and Breeding Institute of Pomology Holovousy Ltd., Hořice, Czech RepublicResearch and Breeding Institute of Pomology Holovousy Ltd., Hořice, Czech RepublicDivision of Crop Protection and Plant Health, Department of Phytobacteriology, Crop Research Institute, Prague, Czech RepublicDivision of Crop Protection and Plant Health, Department of Phytobacteriology, Crop Research Institute, Prague, Czech RepublicThe suitability of different in vitro artificial inoculation methods for testing apple resistance to fire blight was evaluated. Six proliferation MS media containing BAP, TDZ or 2iP were tested to determine favourable conditions for shoot development. Out of the tested cytokinins, only media with BAP proved to be useful for all tested cultivars for artificial inoculation with Erwinia amylovora. Out of three tested inoculation methods, removal of 3 mm of the shoot apex with scissors and transfer of bacterial suspension by pipette (method 1) appeared to be the most suitable technique. This method enabled the determination of susceptibility of two cultivars due to the different speed of fire blight symptom development.https://pps.agriculturejournals.cz/artkey/pps-201501-0001_development-of-a-system-for-testing-apple-resistance-to-erwinia-amylovora-using-in-vitro-culture-techniques.phpfire blightmalus l.cultivarartificial inoculationmediumgrowth regulator |
| spellingShingle | Jiří SEDLÁK František PAPRŠTEIN Josef KORBA Jana ŠILLEROVÁ Development of a system for testing apple resistance to Erwinia amylovora using in vitro culture techniques Plant Protection Science fire blight malus l. cultivar artificial inoculation medium growth regulator |
| title | Development of a system for testing apple resistance to Erwinia amylovora using in vitro culture techniques |
| title_full | Development of a system for testing apple resistance to Erwinia amylovora using in vitro culture techniques |
| title_fullStr | Development of a system for testing apple resistance to Erwinia amylovora using in vitro culture techniques |
| title_full_unstemmed | Development of a system for testing apple resistance to Erwinia amylovora using in vitro culture techniques |
| title_short | Development of a system for testing apple resistance to Erwinia amylovora using in vitro culture techniques |
| title_sort | development of a system for testing apple resistance to erwinia amylovora using in vitro culture techniques |
| topic | fire blight malus l. cultivar artificial inoculation medium growth regulator |
| url | https://pps.agriculturejournals.cz/artkey/pps-201501-0001_development-of-a-system-for-testing-apple-resistance-to-erwinia-amylovora-using-in-vitro-culture-techniques.php |
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