Screening and Identification of Transcription Factors Potentially Regulating <i>Foxl2</i> Expression in <i>Chlamys farreri</i> Ovary
<i>Foxl2</i> is an evolutionarily conserved female sex gene, which is specifically expressed in the ovary and mainly involved in oogenesis and ovarian function maintenance. However, little is known about the mechanism that regulates <i>Foxl2</i> specific expression during the...
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2022-01-01
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| author | Shutong Fan Xixi Li Siyu Lin Yunpeng Li Huixin Ma Zhifeng Zhang Zhenkui Qin |
| author_facet | Shutong Fan Xixi Li Siyu Lin Yunpeng Li Huixin Ma Zhifeng Zhang Zhenkui Qin |
| author_sort | Shutong Fan |
| collection | DOAJ |
| description | <i>Foxl2</i> is an evolutionarily conserved female sex gene, which is specifically expressed in the ovary and mainly involved in oogenesis and ovarian function maintenance. However, little is known about the mechanism that regulates <i>Foxl2</i> specific expression during the ovary development. In the present study, we constructed the gonadal yeast one-hybrid (Y1H) library of <i>Chlamys</i><i>farreri</i> with ovaries and testes at different developmental stages using the Gateway technology. The library capacity was more than 1.36 × 10<sup>7</sup> CFU, and the length of the inserted fragment was 0.75 Kb~2 Kb, which fully met the demand of yeast library screening. The highly transcriptional activity promoter sequence of <i>C. farreri Foxl2</i> (<i>Cf-Foxl2</i>) was determined at −1000~−616 bp by dual-luciferase reporter (DLR) assay and was used as bait to screen possible transcription factors from the Y1H library. Eleven candidate factors, including five unannotated factors, were selected based on Y1H as well as their expressional differences between ovaries and testes and were verified for the first time to be involved in the transcriptional regulation of <i>Cf-Foxl2</i> by RT-qPCR and DLR. Our findings provided valuable data for further studying the specific regulation mechanism of <i>Foxl2</i> in the ovary. |
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| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T01:53:14Z |
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| spelling | doaj.art-af3a4a0e347b4f34a7a8eed307b7bbd62023-11-23T13:01:26ZengMDPI AGBiology2079-77372022-01-0111111310.3390/biology11010113Screening and Identification of Transcription Factors Potentially Regulating <i>Foxl2</i> Expression in <i>Chlamys farreri</i> OvaryShutong Fan0Xixi Li1Siyu Lin2Yunpeng Li3Huixin Ma4Zhifeng Zhang5Zhenkui Qin6Ministry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, ChinaMinistry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, ChinaMinistry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, ChinaMinistry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, ChinaMinistry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, ChinaMinistry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, ChinaMinistry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China<i>Foxl2</i> is an evolutionarily conserved female sex gene, which is specifically expressed in the ovary and mainly involved in oogenesis and ovarian function maintenance. However, little is known about the mechanism that regulates <i>Foxl2</i> specific expression during the ovary development. In the present study, we constructed the gonadal yeast one-hybrid (Y1H) library of <i>Chlamys</i><i>farreri</i> with ovaries and testes at different developmental stages using the Gateway technology. The library capacity was more than 1.36 × 10<sup>7</sup> CFU, and the length of the inserted fragment was 0.75 Kb~2 Kb, which fully met the demand of yeast library screening. The highly transcriptional activity promoter sequence of <i>C. farreri Foxl2</i> (<i>Cf-Foxl2</i>) was determined at −1000~−616 bp by dual-luciferase reporter (DLR) assay and was used as bait to screen possible transcription factors from the Y1H library. Eleven candidate factors, including five unannotated factors, were selected based on Y1H as well as their expressional differences between ovaries and testes and were verified for the first time to be involved in the transcriptional regulation of <i>Cf-Foxl2</i> by RT-qPCR and DLR. Our findings provided valuable data for further studying the specific regulation mechanism of <i>Foxl2</i> in the ovary.https://www.mdpi.com/2079-7737/11/1/113<i>Foxl2</i>gonadsexually dimorphic expressiontranscriptional regulationtranscription factor<i>Chlamys farreri</i> |
| spellingShingle | Shutong Fan Xixi Li Siyu Lin Yunpeng Li Huixin Ma Zhifeng Zhang Zhenkui Qin Screening and Identification of Transcription Factors Potentially Regulating <i>Foxl2</i> Expression in <i>Chlamys farreri</i> Ovary Biology <i>Foxl2</i> gonad sexually dimorphic expression transcriptional regulation transcription factor <i>Chlamys farreri</i> |
| title | Screening and Identification of Transcription Factors Potentially Regulating <i>Foxl2</i> Expression in <i>Chlamys farreri</i> Ovary |
| title_full | Screening and Identification of Transcription Factors Potentially Regulating <i>Foxl2</i> Expression in <i>Chlamys farreri</i> Ovary |
| title_fullStr | Screening and Identification of Transcription Factors Potentially Regulating <i>Foxl2</i> Expression in <i>Chlamys farreri</i> Ovary |
| title_full_unstemmed | Screening and Identification of Transcription Factors Potentially Regulating <i>Foxl2</i> Expression in <i>Chlamys farreri</i> Ovary |
| title_short | Screening and Identification of Transcription Factors Potentially Regulating <i>Foxl2</i> Expression in <i>Chlamys farreri</i> Ovary |
| title_sort | screening and identification of transcription factors potentially regulating i foxl2 i expression in i chlamys farreri i ovary |
| topic | <i>Foxl2</i> gonad sexually dimorphic expression transcriptional regulation transcription factor <i>Chlamys farreri</i> |
| url | https://www.mdpi.com/2079-7737/11/1/113 |
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