Anti-inflammatory effect of gallic acid-conjugated chitooligosaccharides in lipopolysaccharide-stimulated RAW 264.7 macrophages
The aim of the present study is to investigate the anti-inflammatory effect of gallic acid grafted onto COS chains (GA-COS), focusing on the reduction of nitric oxide production, downregulation of inflammatory signals such as inducible nitric oxide synthase (iNOS), gene expression of cytokines such...
| Asıl Yazarlar: | , , , , |
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| Materyal Türü: | Makale |
| Dil: | English |
| Baskı/Yayın Bilgisi: |
Vietnam Ministry of Science and Technology
2024-03-01
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| Seri Bilgileri: | Vietnam Journal of Science, Technology and Engineering |
| Konular: | |
| Online Erişim: | https://vietnamscience.vjst.vn/index.php/vjste/article/view/1190 |
| _version_ | 1827316698284294144 |
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| author | Van-Hoai Bui Hong-Tham N. Vo Thanh-Tuan Duong Se-Kwon Kim Dai-Nghiep Ngo |
| author_facet | Van-Hoai Bui Hong-Tham N. Vo Thanh-Tuan Duong Se-Kwon Kim Dai-Nghiep Ngo |
| author_sort | Van-Hoai Bui |
| collection | DOAJ |
| description |
The aim of the present study is to investigate the anti-inflammatory effect of gallic acid grafted onto COS chains (GA-COS), focusing on the reduction of nitric oxide production, downregulation of inflammatory signals such as inducible nitric oxide synthase (iNOS), gene expression of cytokines such as TNF-α, IL-1β, IL-6, and nuclear factor kappa B (NF-κB) signalling, including the p50 and p65 subunits. The anti-inflammatory effect is mediated through the reduction of nitric oxide production and downregulation of inflammatory proteins such as inducible nitric oxide synthase (iNOS), gene expression of cytokines like TNF-α, IL-1β, IL-6, and nuclear factor kappa B (NF-κB) signalling, including the p50 and p65 subunits. Target proteins were identified by western blot analysis with specific monoclonal antibodies. The levels of gene expression were determined by the RT-PCR method. The results demonstrate that GA-COS effectively reduces nitric oxide generation and downregulates iNOS protein and cytokine expression and NF-κB signalling in lipopolysaccharide (LPS) -induced RAW 264.7 cells. GA-COS exhibits significantly enhanced anti-inflammatory activity compared to the free chitooligosaccharide chain. This study lays the groundwork for future research to demonstrate that GACOS holds significant potential as a novel compound for the prevention of inflammatory diseases.
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| first_indexed | 2024-04-24T23:19:50Z |
| format | Article |
| id | doaj.art-af3c66a9b1ed4c129dd6ac3826131b0a |
| institution | Directory Open Access Journal |
| issn | 2525-2461 2615-9937 |
| language | English |
| last_indexed | 2024-04-24T23:19:50Z |
| publishDate | 2024-03-01 |
| publisher | Vietnam Ministry of Science and Technology |
| record_format | Article |
| series | Vietnam Journal of Science, Technology and Engineering |
| spelling | doaj.art-af3c66a9b1ed4c129dd6ac3826131b0a2024-03-16T23:50:07ZengVietnam Ministry of Science and TechnologyVietnam Journal of Science, Technology and Engineering2525-24612615-99372024-03-0166110.31276/VJSTE.66(1).96-103Anti-inflammatory effect of gallic acid-conjugated chitooligosaccharides in lipopolysaccharide-stimulated RAW 264.7 macrophagesVan-Hoai BuiHong-Tham N. VoThanh-Tuan DuongSe-Kwon KimDai-Nghiep Ngo The aim of the present study is to investigate the anti-inflammatory effect of gallic acid grafted onto COS chains (GA-COS), focusing on the reduction of nitric oxide production, downregulation of inflammatory signals such as inducible nitric oxide synthase (iNOS), gene expression of cytokines such as TNF-α, IL-1β, IL-6, and nuclear factor kappa B (NF-κB) signalling, including the p50 and p65 subunits. The anti-inflammatory effect is mediated through the reduction of nitric oxide production and downregulation of inflammatory proteins such as inducible nitric oxide synthase (iNOS), gene expression of cytokines like TNF-α, IL-1β, IL-6, and nuclear factor kappa B (NF-κB) signalling, including the p50 and p65 subunits. Target proteins were identified by western blot analysis with specific monoclonal antibodies. The levels of gene expression were determined by the RT-PCR method. The results demonstrate that GA-COS effectively reduces nitric oxide generation and downregulates iNOS protein and cytokine expression and NF-κB signalling in lipopolysaccharide (LPS) -induced RAW 264.7 cells. GA-COS exhibits significantly enhanced anti-inflammatory activity compared to the free chitooligosaccharide chain. This study lays the groundwork for future research to demonstrate that GACOS holds significant potential as a novel compound for the prevention of inflammatory diseases. https://vietnamscience.vjst.vn/index.php/vjste/article/view/1190anti-inflammatory activitychitooligosaccharidechitooligosaccharide conjugated gallic acidnitric oxideRAW 264.7 macrophage cells |
| spellingShingle | Van-Hoai Bui Hong-Tham N. Vo Thanh-Tuan Duong Se-Kwon Kim Dai-Nghiep Ngo Anti-inflammatory effect of gallic acid-conjugated chitooligosaccharides in lipopolysaccharide-stimulated RAW 264.7 macrophages Vietnam Journal of Science, Technology and Engineering anti-inflammatory activity chitooligosaccharide chitooligosaccharide conjugated gallic acid nitric oxide RAW 264.7 macrophage cells |
| title | Anti-inflammatory effect of gallic acid-conjugated chitooligosaccharides in lipopolysaccharide-stimulated RAW 264.7 macrophages |
| title_full | Anti-inflammatory effect of gallic acid-conjugated chitooligosaccharides in lipopolysaccharide-stimulated RAW 264.7 macrophages |
| title_fullStr | Anti-inflammatory effect of gallic acid-conjugated chitooligosaccharides in lipopolysaccharide-stimulated RAW 264.7 macrophages |
| title_full_unstemmed | Anti-inflammatory effect of gallic acid-conjugated chitooligosaccharides in lipopolysaccharide-stimulated RAW 264.7 macrophages |
| title_short | Anti-inflammatory effect of gallic acid-conjugated chitooligosaccharides in lipopolysaccharide-stimulated RAW 264.7 macrophages |
| title_sort | anti inflammatory effect of gallic acid conjugated chitooligosaccharides in lipopolysaccharide stimulated raw 264 7 macrophages |
| topic | anti-inflammatory activity chitooligosaccharide chitooligosaccharide conjugated gallic acid nitric oxide RAW 264.7 macrophage cells |
| url | https://vietnamscience.vjst.vn/index.php/vjste/article/view/1190 |
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