Results of investigation of ticks in Volga river delta (Astrakhan region, 2017) for Crimean-Congo hemorrhagic fever virus (<i>Nairoviridae</i>, <i>Orthonairovirus</i>, CCHFV) and other tick-borne arboviruses
Introduction. There are natural foci of Crimean-Congo hemorrhagic fever (CCHF) that vectored by Hyalomma marginatum ticks in Volga river delta (Astrakhan region, South of Russia). The circulation of Dhori virus (DHOV) (Thogotovirus: Orthomyxoviridae) has been also shown here. We hypothesized that ot...
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| Format: | Article |
| Language: | English |
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Central Research Institute for Epidemiology
2019-10-01
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| Series: | Вопросы вирусологии |
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| Online Access: | https://virusjour.crie.ru/jour/article/viewFile/13/13 |
| _version_ | 1797782296788467712 |
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| author | E. V. Vakalova A. M. Butenko T. V. Vishnevskaya T. E. Dorofeeva A. K. Gitelman L. N. Kulikova D. K. Lvov S. V. Alkhovsky |
| author_facet | E. V. Vakalova A. M. Butenko T. V. Vishnevskaya T. E. Dorofeeva A. K. Gitelman L. N. Kulikova D. K. Lvov S. V. Alkhovsky |
| author_sort | E. V. Vakalova |
| collection | DOAJ |
| description | Introduction. There are natural foci of Crimean-Congo hemorrhagic fever (CCHF) that vectored by Hyalomma marginatum ticks in Volga river delta (Astrakhan region, South of Russia). The circulation of Dhori virus (DHOV) (Thogotovirus: Orthomyxoviridae) has been also shown here. We hypothesized that other tick-borne arboviruses are also likely to circulate in the region. In particular, Bhanja virus (Phlebovirus: Phenuiviridae), Wad Medani virus (Orbivirus: Reoviridae), and Tamdy virus (Orthonairovirus: Nairoviridae), which were found to circulate in neighboring regions and are vectored by Haemaphysalis spp., Dermacenter spp., and Hyalomma spp. ticks.
Goals and objectives. The aim of the study was to examine ixodid ticks in Volga river delta for the presence of CCHFV, DHOV, Bhanja virus, Wad Medani virus, and Tamdy virus.
Material and methods. Ticks were collected in Volga river delta in 2017. We used molecular genetic methods for the detection and analysis of nucleic acids (PCR, sequencing, phylogenetic analysis).
Results. We detect CCHFV and DHOV RNA in H. marginatum ticks. The rate of infected H. marginatum ticks was 1.98% for CCHFV and 0.4% for DHOV. The results of genetic analysis showed that found DHOV strains are almost identical (99- 100% in the M gene) and forms a separate genetic lineage alongside of Batken virus from Central Asia. At the same time, Bhanja virus, Wad Medani virus, and Tamdy virus were not found in ticks, collected in this region.
Conclusions. DHOV is circulating in the natural foci of CCHF in the Volga river delta. The ratio of infection of H. marginatum with CCHFV and DHOV was determined for the first time. |
| first_indexed | 2024-03-13T00:08:59Z |
| format | Article |
| id | doaj.art-af4034f961c841aea1948d446e1d344e |
| institution | Directory Open Access Journal |
| issn | 0507-4088 2411-2097 |
| language | English |
| last_indexed | 2024-03-13T00:08:59Z |
| publishDate | 2019-10-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Вопросы вирусологии |
| spelling | doaj.art-af4034f961c841aea1948d446e1d344e2023-07-12T19:59:35ZengCentral Research Institute for EpidemiologyВопросы вирусологии0507-40882411-20972019-10-0164522122810.36233/0507-4088-2019-64-5-221-22812Results of investigation of ticks in Volga river delta (Astrakhan region, 2017) for Crimean-Congo hemorrhagic fever virus (<i>Nairoviridae</i>, <i>Orthonairovirus</i>, CCHFV) and other tick-borne arbovirusesE. V. Vakalova0https://orcid.org/0000-0001-6121-5900A. M. Butenko1https://orcid.org/0000-0001-6152-5685T. V. Vishnevskaya2https://orcid.org/0000-0002-6963-8681T. E. Dorofeeva3https://orcid.org/0000-0001-8846-0092A. K. Gitelman4https://orcid.org/0000-0002-8410-2332L. N. Kulikova5https://orcid.org/0000-0003-3066-3430D. K. Lvov6https://orcid.org/0000-0001-8176-6582S. V. Alkhovsky7https://orcid.org/0000-0001-6913-5841Astrakhan Anti-plague StationD.I. Ivanovsky Institute of Virology National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F.GamaleyaD.I. Ivanovsky Institute of Virology National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F.GamaleyaD.I. Ivanovsky Institute of Virology National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F.GamaleyaD.I. Ivanovsky Institute of Virology National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F.GamaleyaCenter of Hygiene and Epidemiology in Astrakhan regionD.I. Ivanovsky Institute of Virology National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F.GamaleyaD.I. Ivanovsky Institute of Virology National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F.GamaleyaIntroduction. There are natural foci of Crimean-Congo hemorrhagic fever (CCHF) that vectored by Hyalomma marginatum ticks in Volga river delta (Astrakhan region, South of Russia). The circulation of Dhori virus (DHOV) (Thogotovirus: Orthomyxoviridae) has been also shown here. We hypothesized that other tick-borne arboviruses are also likely to circulate in the region. In particular, Bhanja virus (Phlebovirus: Phenuiviridae), Wad Medani virus (Orbivirus: Reoviridae), and Tamdy virus (Orthonairovirus: Nairoviridae), which were found to circulate in neighboring regions and are vectored by Haemaphysalis spp., Dermacenter spp., and Hyalomma spp. ticks. Goals and objectives. The aim of the study was to examine ixodid ticks in Volga river delta for the presence of CCHFV, DHOV, Bhanja virus, Wad Medani virus, and Tamdy virus. Material and methods. Ticks were collected in Volga river delta in 2017. We used molecular genetic methods for the detection and analysis of nucleic acids (PCR, sequencing, phylogenetic analysis). Results. We detect CCHFV and DHOV RNA in H. marginatum ticks. The rate of infected H. marginatum ticks was 1.98% for CCHFV and 0.4% for DHOV. The results of genetic analysis showed that found DHOV strains are almost identical (99- 100% in the M gene) and forms a separate genetic lineage alongside of Batken virus from Central Asia. At the same time, Bhanja virus, Wad Medani virus, and Tamdy virus were not found in ticks, collected in this region. Conclusions. DHOV is circulating in the natural foci of CCHF in the Volga river delta. The ratio of infection of H. marginatum with CCHFV and DHOV was determined for the first time.https://virusjour.crie.ru/jour/article/viewFile/13/13hyalomma marginatumdermacenter marginatus |
| spellingShingle | E. V. Vakalova A. M. Butenko T. V. Vishnevskaya T. E. Dorofeeva A. K. Gitelman L. N. Kulikova D. K. Lvov S. V. Alkhovsky Results of investigation of ticks in Volga river delta (Astrakhan region, 2017) for Crimean-Congo hemorrhagic fever virus (<i>Nairoviridae</i>, <i>Orthonairovirus</i>, CCHFV) and other tick-borne arboviruses Вопросы вирусологии hyalomma marginatum dermacenter marginatus |
| title | Results of investigation of ticks in Volga river delta (Astrakhan region, 2017) for Crimean-Congo hemorrhagic fever virus (<i>Nairoviridae</i>, <i>Orthonairovirus</i>, CCHFV) and other tick-borne arboviruses |
| title_full | Results of investigation of ticks in Volga river delta (Astrakhan region, 2017) for Crimean-Congo hemorrhagic fever virus (<i>Nairoviridae</i>, <i>Orthonairovirus</i>, CCHFV) and other tick-borne arboviruses |
| title_fullStr | Results of investigation of ticks in Volga river delta (Astrakhan region, 2017) for Crimean-Congo hemorrhagic fever virus (<i>Nairoviridae</i>, <i>Orthonairovirus</i>, CCHFV) and other tick-borne arboviruses |
| title_full_unstemmed | Results of investigation of ticks in Volga river delta (Astrakhan region, 2017) for Crimean-Congo hemorrhagic fever virus (<i>Nairoviridae</i>, <i>Orthonairovirus</i>, CCHFV) and other tick-borne arboviruses |
| title_short | Results of investigation of ticks in Volga river delta (Astrakhan region, 2017) for Crimean-Congo hemorrhagic fever virus (<i>Nairoviridae</i>, <i>Orthonairovirus</i>, CCHFV) and other tick-borne arboviruses |
| title_sort | results of investigation of ticks in volga river delta astrakhan region 2017 for crimean congo hemorrhagic fever virus i nairoviridae i i orthonairovirus i cchfv and other tick borne arboviruses |
| topic | hyalomma marginatum dermacenter marginatus |
| url | https://virusjour.crie.ru/jour/article/viewFile/13/13 |
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