Allelic Discrimination of Vitamin D Receptor Polymorphisms and Risk of Type 2 Diabetes Mellitus: A Case-Controlled Study
(1) Background: Type 2 diabetes mellitus (T2DM) is one of the rapidly growing healthcare problems, and several vitamin D receptor (VDR) polymorphisms seem to modulate the risk of T2DM. Our research was designed to investigate the allelic discrimination of VDR polymorphisms and T2DM occurrence risk....
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MDPI AG
2023-02-01
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| Online Access: | https://www.mdpi.com/2227-9032/11/4/485 |
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| author | Amal Ahmed Mohammed Dina M. Abo El-Matty Rola Abdel-Azeem Khaled Raafat Mona A. Hussein Amira R. El-Ansary Wael Hafez Hatem Ahmed Hassan Nourelhuda Ahmed Nassar Nora Mahmoud Selim Doaa Ghaith Amal A. El Kholy Soha M. Abd El Salam Fatme Al Anouti Alaa S. Wahba |
| author_facet | Amal Ahmed Mohammed Dina M. Abo El-Matty Rola Abdel-Azeem Khaled Raafat Mona A. Hussein Amira R. El-Ansary Wael Hafez Hatem Ahmed Hassan Nourelhuda Ahmed Nassar Nora Mahmoud Selim Doaa Ghaith Amal A. El Kholy Soha M. Abd El Salam Fatme Al Anouti Alaa S. Wahba |
| author_sort | Amal Ahmed Mohammed |
| collection | DOAJ |
| description | (1) Background: Type 2 diabetes mellitus (T2DM) is one of the rapidly growing healthcare problems, and several vitamin D receptor (VDR) polymorphisms seem to modulate the risk of T2DM. Our research was designed to investigate the allelic discrimination of VDR polymorphisms and T2DM occurrence risk. (2) Methods: This case-control research included 156 patients with T2DM and 145 healthy control subjects. Most of the study population were males 56.6% vs. 62.8% in the case and control groups, respectively. Genotyping for VDR single nucleotide polymorphisms (SNPs), rs228570 (Fok1), rs7975232 (Apa1), and rs1544410 (Bsm1) was compared between both groups. (3) Results: There was a negative link between vitamin D levels and insulin sensitivity. A significant difference was noted in the allelic discrimination of VDR polymorphism rs228570 and rs1544410 between the study groups (<i>p</i> < 0.001). No difference was observed in the allelic discrimination of VDR polymorphism rs7975232 between the groups (<i>p</i> = 0.063). Moreover, T2DM patients had significantly higher levels of fasting blood sugar (FBS), glycated hemoglobin HbA1c, 2-h post-prandial blood sugar (PP), serum glutamic oxaloacetic transaminase (SGOT), serum glutamic-pyruvic transaminase (SGPT), total cholesterol, and triglycerides (<i>p</i> < 0.001), while High-Density Lipoprotein (HDL) Cholesterol (HDL-C) was significantly decreased (<i>p</i> = 0.006). (4) Conclusions: VDR polymorphisms had a positive association with T2DM risk among the Egyptian population. Further large-scale research using deep sequencing of samples is strongly urged to investigate different vitamin D gene variants and interactions, as well as the influence of vitamin D on T2DM. |
| first_indexed | 2024-03-11T08:46:08Z |
| format | Article |
| id | doaj.art-af46cc92e583463e950904ee9ea63e8c |
| institution | Directory Open Access Journal |
| issn | 2227-9032 |
| language | English |
| last_indexed | 2024-03-11T08:46:08Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
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| series | Healthcare |
| spelling | doaj.art-af46cc92e583463e950904ee9ea63e8c2023-11-16T20:45:54ZengMDPI AGHealthcare2227-90322023-02-0111448510.3390/healthcare11040485Allelic Discrimination of Vitamin D Receptor Polymorphisms and Risk of Type 2 Diabetes Mellitus: A Case-Controlled StudyAmal Ahmed Mohammed0Dina M. Abo El-Matty1Rola Abdel-Azeem2Khaled Raafat3Mona A. Hussein4Amira R. El-Ansary5Wael Hafez6Hatem Ahmed Hassan7Nourelhuda Ahmed Nassar8Nora Mahmoud Selim9Doaa Ghaith10Amal A. El Kholy11Soha M. Abd El Salam12Fatme Al Anouti13Alaa S. Wahba14Department of Biochemistry and Molecular Biology, National Hepatology and Tropical Medicine Research Institute (NHTMRI), Cairo 11796, EgyptDepartment of Biochemistry, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, EgyptDepartment of Biochemistry, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, EgyptDepartment of Internal Medicine, Faculty of Medicine, Ain Shams University, Cairo11566, EgyptDepartment of Internal Medicine, National Institute of Diabetes and Endocrinology, Cairo 11562, EgyptDepartment of Internal Medicine, Faculty of Medicine, Misr University for Science and Technology, Giza 12577, EgyptDepartment of Internal Medicine, The National Research Centre, Cairo 12622, EgyptDepartment of Internal Medicine and Gastroenterology, Faculty of Medicine, Minia University, Minia 61519, EgyptClinical Pathology Department, Elsahel Teaching Hospital, Cairo 11697, EgyptDepartment of Clinical and Chemical Pathology Department, Faculty of Medicine, Cairo University, Cairo 12613, EgyptDepartment of Clinical and Chemical Pathology Department, Faculty of Medicine, Cairo University, Cairo 12613, EgyptDepartment of Clinical Pharmacy, Faculty of Pharmacy, Ain Shams University, Cairo 11566, EgyptDepartment of Medical Microbiology and Immunology, Faculty of Medicine, Suez University, Suez 43512, EgyptDepartment of Health Sciences, College of Natural and Health Sciences, Zayed University, Abu Dhabi 144534, United Arab EmiratesDepartment of Biochemistry, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt(1) Background: Type 2 diabetes mellitus (T2DM) is one of the rapidly growing healthcare problems, and several vitamin D receptor (VDR) polymorphisms seem to modulate the risk of T2DM. Our research was designed to investigate the allelic discrimination of VDR polymorphisms and T2DM occurrence risk. (2) Methods: This case-control research included 156 patients with T2DM and 145 healthy control subjects. Most of the study population were males 56.6% vs. 62.8% in the case and control groups, respectively. Genotyping for VDR single nucleotide polymorphisms (SNPs), rs228570 (Fok1), rs7975232 (Apa1), and rs1544410 (Bsm1) was compared between both groups. (3) Results: There was a negative link between vitamin D levels and insulin sensitivity. A significant difference was noted in the allelic discrimination of VDR polymorphism rs228570 and rs1544410 between the study groups (<i>p</i> < 0.001). No difference was observed in the allelic discrimination of VDR polymorphism rs7975232 between the groups (<i>p</i> = 0.063). Moreover, T2DM patients had significantly higher levels of fasting blood sugar (FBS), glycated hemoglobin HbA1c, 2-h post-prandial blood sugar (PP), serum glutamic oxaloacetic transaminase (SGOT), serum glutamic-pyruvic transaminase (SGPT), total cholesterol, and triglycerides (<i>p</i> < 0.001), while High-Density Lipoprotein (HDL) Cholesterol (HDL-C) was significantly decreased (<i>p</i> = 0.006). (4) Conclusions: VDR polymorphisms had a positive association with T2DM risk among the Egyptian population. Further large-scale research using deep sequencing of samples is strongly urged to investigate different vitamin D gene variants and interactions, as well as the influence of vitamin D on T2DM.https://www.mdpi.com/2227-9032/11/4/485vitamin D receptorpolymorphismallelic discriminationT2DM |
| spellingShingle | Amal Ahmed Mohammed Dina M. Abo El-Matty Rola Abdel-Azeem Khaled Raafat Mona A. Hussein Amira R. El-Ansary Wael Hafez Hatem Ahmed Hassan Nourelhuda Ahmed Nassar Nora Mahmoud Selim Doaa Ghaith Amal A. El Kholy Soha M. Abd El Salam Fatme Al Anouti Alaa S. Wahba Allelic Discrimination of Vitamin D Receptor Polymorphisms and Risk of Type 2 Diabetes Mellitus: A Case-Controlled Study Healthcare vitamin D receptor polymorphism allelic discrimination T2DM |
| title | Allelic Discrimination of Vitamin D Receptor Polymorphisms and Risk of Type 2 Diabetes Mellitus: A Case-Controlled Study |
| title_full | Allelic Discrimination of Vitamin D Receptor Polymorphisms and Risk of Type 2 Diabetes Mellitus: A Case-Controlled Study |
| title_fullStr | Allelic Discrimination of Vitamin D Receptor Polymorphisms and Risk of Type 2 Diabetes Mellitus: A Case-Controlled Study |
| title_full_unstemmed | Allelic Discrimination of Vitamin D Receptor Polymorphisms and Risk of Type 2 Diabetes Mellitus: A Case-Controlled Study |
| title_short | Allelic Discrimination of Vitamin D Receptor Polymorphisms and Risk of Type 2 Diabetes Mellitus: A Case-Controlled Study |
| title_sort | allelic discrimination of vitamin d receptor polymorphisms and risk of type 2 diabetes mellitus a case controlled study |
| topic | vitamin D receptor polymorphism allelic discrimination T2DM |
| url | https://www.mdpi.com/2227-9032/11/4/485 |
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