Expansion of CRISPR Targeting Sites Using an Integrated Gene-Editing System in <i>Apis mellifera</i>

Expansion of CRISPR Targeting Sites Using an Integrated Gene-Editing System in <i>Apis mellifera</i>

CRISPR/Cas9, a predominant gene-editing tool, has been utilised to dissect the gene function in <i>Apis mellifera</i>. However, only the genomic region containing NGG PAM could be recognised and edited in <i>A. mellifera</i>, seriously hampering the application of CRISPR tech...

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Bibliographic Details
Main Authors: Liqiang Liang, Zhenghanqing Li, Qiufang Li, Xiuxiu Wang, Songkun Su, Hongyi Nie
Format: Article
Language:English
Published: MDPI AG 2021-10-01
Series:Insects
Subjects:
CRISPR/Cas9
gene editing
<i>Apis mellifera</i>
editing scope
NGG
Online Access:https://www.mdpi.com/2075-4450/12/10/954

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