Plant Regeneration from Leaf Explants of the Medicinal Herb <i>Wedelia chinensis</i>
<i>Wedelia chinensis</i>, belonging to the Asteraceae family, has been used in folk medicine in East and South Asia for the treatment of common inflammatory diseases and protection against liver toxicity. Previously, in vitro propagation through different tissue explants has been reporte...
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| Format: | Article |
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MDPI AG
2021-10-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/7/10/407 |
| _version_ | 1797514465769422848 |
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| author | Yung-Ting Tsai Kin-Ying To |
| author_facet | Yung-Ting Tsai Kin-Ying To |
| author_sort | Yung-Ting Tsai |
| collection | DOAJ |
| description | <i>Wedelia chinensis</i>, belonging to the Asteraceae family, has been used in folk medicine in East and South Asia for the treatment of common inflammatory diseases and protection against liver toxicity. Previously, in vitro propagation through different tissue explants has been reported, including through nodal segments, axillary buds, and shoot tips, whereas leaf segments failed to proliferate. Here, we report on the in vitro propagation of <i>W. chinensis</i> by culturing young leaf explants in MS medium supplemented with 0.5 mg/L α-naphthaleneacetic acid (NAA), 0.75 mg/L thidiazuron (TDZ), 1 mg/L gibberellic acid (GA<sub>3</sub>), 3.75 mg/L adenine, 3% sucrose, and 0.8% agar at pH 5.8. Calli were observed in all explants derived from the youngest top two leaves, and the average percentage of shoot regeneration was 23% from three independent experiments. Then, several shoots were excised, transferred onto MS basal medium supplemented with 3% sucrose and 0.8% agar at pH 5.8, and cultured in a growth chamber for 1 to 2 months. Roots were easily induced. Finally, plantlets carrying shoots and roots were transferred into soil, and all of them grew healthily in a greenhouse. No morphological variation was observed between the regenerated plantlets and the donor wild-type plants. In addition, we also established root cultures of <i>W. chinensis</i> in culture medium (MS medium, 3 mg/L NAA, 3% sucrose, pH 5.8) with or without 0.8% agar. To the best of our knowledge, this is the first paper reporting plant regeneration from leaf explants in the herbal plant <i>W. chinensis</i>. |
| first_indexed | 2024-03-10T06:32:01Z |
| format | Article |
| id | doaj.art-af4e0b35f8694ae1b004032f3144b5bf |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-10T06:32:01Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-af4e0b35f8694ae1b004032f3144b5bf2023-11-22T18:27:52ZengMDPI AGHorticulturae2311-75242021-10-0171040710.3390/horticulturae7100407Plant Regeneration from Leaf Explants of the Medicinal Herb <i>Wedelia chinensis</i>Yung-Ting Tsai0Kin-Ying To1Agricultural Biotechnology Research Center, Academia Sinica, Taipei 115, TaiwanAgricultural Biotechnology Research Center, Academia Sinica, Taipei 115, Taiwan<i>Wedelia chinensis</i>, belonging to the Asteraceae family, has been used in folk medicine in East and South Asia for the treatment of common inflammatory diseases and protection against liver toxicity. Previously, in vitro propagation through different tissue explants has been reported, including through nodal segments, axillary buds, and shoot tips, whereas leaf segments failed to proliferate. Here, we report on the in vitro propagation of <i>W. chinensis</i> by culturing young leaf explants in MS medium supplemented with 0.5 mg/L α-naphthaleneacetic acid (NAA), 0.75 mg/L thidiazuron (TDZ), 1 mg/L gibberellic acid (GA<sub>3</sub>), 3.75 mg/L adenine, 3% sucrose, and 0.8% agar at pH 5.8. Calli were observed in all explants derived from the youngest top two leaves, and the average percentage of shoot regeneration was 23% from three independent experiments. Then, several shoots were excised, transferred onto MS basal medium supplemented with 3% sucrose and 0.8% agar at pH 5.8, and cultured in a growth chamber for 1 to 2 months. Roots were easily induced. Finally, plantlets carrying shoots and roots were transferred into soil, and all of them grew healthily in a greenhouse. No morphological variation was observed between the regenerated plantlets and the donor wild-type plants. In addition, we also established root cultures of <i>W. chinensis</i> in culture medium (MS medium, 3 mg/L NAA, 3% sucrose, pH 5.8) with or without 0.8% agar. To the best of our knowledge, this is the first paper reporting plant regeneration from leaf explants in the herbal plant <i>W. chinensis</i>.https://www.mdpi.com/2311-7524/7/10/407in vitro propagationmedicinal plantplant tissue cultureroot culture |
| spellingShingle | Yung-Ting Tsai Kin-Ying To Plant Regeneration from Leaf Explants of the Medicinal Herb <i>Wedelia chinensis</i> Horticulturae in vitro propagation medicinal plant plant tissue culture root culture |
| title | Plant Regeneration from Leaf Explants of the Medicinal Herb <i>Wedelia chinensis</i> |
| title_full | Plant Regeneration from Leaf Explants of the Medicinal Herb <i>Wedelia chinensis</i> |
| title_fullStr | Plant Regeneration from Leaf Explants of the Medicinal Herb <i>Wedelia chinensis</i> |
| title_full_unstemmed | Plant Regeneration from Leaf Explants of the Medicinal Herb <i>Wedelia chinensis</i> |
| title_short | Plant Regeneration from Leaf Explants of the Medicinal Herb <i>Wedelia chinensis</i> |
| title_sort | plant regeneration from leaf explants of the medicinal herb i wedelia chinensis i |
| topic | in vitro propagation medicinal plant plant tissue culture root culture |
| url | https://www.mdpi.com/2311-7524/7/10/407 |
| work_keys_str_mv | AT yungtingtsai plantregenerationfromleafexplantsofthemedicinalherbiwedeliachinensisi AT kinyingto plantregenerationfromleafexplantsofthemedicinalherbiwedeliachinensisi |