Candida on oral cavity of pediatric individuals with ALL and its susceptibility to nystatin and amphotericin B
Objective: The aim of this study was to evaluate the prevalence of Candida colonizationon oral cavity of pediatric individuals with acute lymphocytic leukemia (ALL) and its susceptibility/resistance to nystatin and amphotericin B. Methods: This was a cross sectional study with observational, descrip...
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| Format: | Article |
| Language: | English |
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Faculdade São Leopoldo Mandic
2019-05-01
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| Series: | RGO: Revista Gaúcha de Odontologia |
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| Online Access: | https://www.scielo.br/scielo.php?script=sci_arttext&pid=S1981-86372019000100309&lng=en&nrm=iso&tlng=en |
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| author | Larissa Cavalcanti MONTEIRO Isabella Lima Arrais RIBEIRO Roberta Ferreti Bonan Dantas BATISTA Panmella Pereira MACIEL Ana Maria Gondim VALENÇA Ana Carolina Bernardes DULGHEROFF4 Joelma Rodrigues SOUZA Yuri Wanderley CAVALCANTI1 Lucio Roberto Cançado CASTELLANO Paulo Rogério Ferreti BONAN |
| author_facet | Larissa Cavalcanti MONTEIRO Isabella Lima Arrais RIBEIRO Roberta Ferreti Bonan Dantas BATISTA Panmella Pereira MACIEL Ana Maria Gondim VALENÇA Ana Carolina Bernardes DULGHEROFF4 Joelma Rodrigues SOUZA Yuri Wanderley CAVALCANTI1 Lucio Roberto Cançado CASTELLANO Paulo Rogério Ferreti BONAN |
| author_sort | Larissa Cavalcanti MONTEIRO |
| collection | DOAJ |
| description | Objective: The aim of this study was to evaluate the prevalence of Candida colonizationon oral cavity of pediatric individuals with acute lymphocytic leukemia (ALL) and its susceptibility/resistance to nystatin and amphotericin B. Methods: This was a cross sectional study with observational, descriptive and analytic approach. Saliva was collected from40 individuals diagnosed with ALL and from40 healthy subjects, as a comparative group, matched by age and gender with ALL group. The mean age for both groups were 8 years-old. The isolation and identification of the Candidaspecies were performed using the CHROMagarCandidaTM and confirmed by polymerase chain reaction. The samples were subjected to antifungal susceptibility by microdilution assay for nystatin and amphotericin B. Salivary alterations and chemotherapy-induced oralmucositis were evaluated using modifiedOral Assessment Guide. Results: The positivity to Candida was higher inALL individuals (32.5%,13/40)than in a comparative group(2.5%, 1/40) (p<0.001). Candida albicans was the most prevalent strain (86.6%). The mucositis was directly associated with positive Candidacolonization (p=0.017) in the ALL group but not related with salivary alterations (p= 0.479). Six strains of C. albicans (54.5%), on ALL group, were resistant to nystatin and all strains were not susceptible to amphotericin B. Conclusion: Candida colonization was associated with ALL condition and with oral mucositis in these individuals. C. albicans was the prevalent strain and most samples were resistant to antifungal agents tested, nystatin and amphotericin B. |
| first_indexed | 2024-04-14T06:56:23Z |
| format | Article |
| id | doaj.art-af4e0b9f28c249598c240fd616a73f86 |
| institution | Directory Open Access Journal |
| issn | 1981-8637 |
| language | English |
| last_indexed | 2024-04-14T06:56:23Z |
| publishDate | 2019-05-01 |
| publisher | Faculdade São Leopoldo Mandic |
| record_format | Article |
| series | RGO: Revista Gaúcha de Odontologia |
| spelling | doaj.art-af4e0b9f28c249598c240fd616a73f862022-12-22T02:06:53ZengFaculdade São Leopoldo MandicRGO: Revista Gaúcha de Odontologia1981-86372019-05-0167e20190018https://doi.org/10.1590/1981-86372019000183558Candida on oral cavity of pediatric individuals with ALL and its susceptibility to nystatin and amphotericin BLarissa Cavalcanti MONTEIRO0https://orcid.org/0000-0002-7349-0878Isabella Lima Arrais RIBEIRO1https://orcid.org/0000-0001-6538-6811Roberta Ferreti Bonan Dantas BATISTA2https://orcid.org/0000-0001-8737-6032Panmella Pereira MACIEL3https://orcid.org/0000-0003-0168-5833Ana Maria Gondim VALENÇA4https://orcid.org/0000-0001-8460-3981Ana Carolina Bernardes DULGHEROFF4 5https://orcid.org/0000-0003-2126-8623Joelma Rodrigues SOUZA6https://orcid.org/0000-0002-1456-9009Yuri Wanderley CAVALCANTI1 7https://orcid.org/0000-0002-3570-9904Lucio Roberto Cançado CASTELLANO8https://orcid.org/0000-0003-0851-5298Paulo Rogério Ferreti BONAN9https://orcid.org/0000-0002-4449-4343Universidade Federal da Paraíba, Programa de Pós-Graduação em OdontologiaUniversidade Federal da Paraíba, Programa de Pós-Graduação em OdontologiaUniversidade Federal da Paraíba, Programa de Pós-Graduação em OdontologiaUniversidade Federal da Paraíba, Faculdade de OdontologiaUniversidade Federal da Paraíba, Departamento de Odontologia Clínica e SocialUniversidade Federal da Paraíba, Escola Técnica de Saúde, Grupo de Estudos e Pesquisas em Imunologia Humana Universidade Federal da Paraíba, Escola Técnica de Saúde, Grupo de Estudos e Pesquisas em Imunologia Humana Universidade Federal da Paraíba, Programa de Pós-Graduação em OdontologiaUniversidade Federal da Paraíba, Programa de Pós-Graduação em OdontologiaUniversidade Federal da Paraíba, Programa de Pós-Graduação em OdontologiaObjective: The aim of this study was to evaluate the prevalence of Candida colonizationon oral cavity of pediatric individuals with acute lymphocytic leukemia (ALL) and its susceptibility/resistance to nystatin and amphotericin B. Methods: This was a cross sectional study with observational, descriptive and analytic approach. Saliva was collected from40 individuals diagnosed with ALL and from40 healthy subjects, as a comparative group, matched by age and gender with ALL group. The mean age for both groups were 8 years-old. The isolation and identification of the Candidaspecies were performed using the CHROMagarCandidaTM and confirmed by polymerase chain reaction. The samples were subjected to antifungal susceptibility by microdilution assay for nystatin and amphotericin B. Salivary alterations and chemotherapy-induced oralmucositis were evaluated using modifiedOral Assessment Guide. Results: The positivity to Candida was higher inALL individuals (32.5%,13/40)than in a comparative group(2.5%, 1/40) (p<0.001). Candida albicans was the most prevalent strain (86.6%). The mucositis was directly associated with positive Candidacolonization (p=0.017) in the ALL group but not related with salivary alterations (p= 0.479). Six strains of C. albicans (54.5%), on ALL group, were resistant to nystatin and all strains were not susceptible to amphotericin B. Conclusion: Candida colonization was associated with ALL condition and with oral mucositis in these individuals. C. albicans was the prevalent strain and most samples were resistant to antifungal agents tested, nystatin and amphotericin B.https://www.scielo.br/scielo.php?script=sci_arttext&pid=S1981-86372019000100309&lng=en&nrm=iso&tlng=encandidaprecursor cell lymphoblastic leukemia-lymphomaprevalence |
| spellingShingle | Larissa Cavalcanti MONTEIRO Isabella Lima Arrais RIBEIRO Roberta Ferreti Bonan Dantas BATISTA Panmella Pereira MACIEL Ana Maria Gondim VALENÇA Ana Carolina Bernardes DULGHEROFF4 Joelma Rodrigues SOUZA Yuri Wanderley CAVALCANTI1 Lucio Roberto Cançado CASTELLANO Paulo Rogério Ferreti BONAN Candida on oral cavity of pediatric individuals with ALL and its susceptibility to nystatin and amphotericin B RGO: Revista Gaúcha de Odontologia candida precursor cell lymphoblastic leukemia-lymphoma prevalence |
| title | Candida on oral cavity of pediatric individuals with ALL and its susceptibility to nystatin and amphotericin B |
| title_full | Candida on oral cavity of pediatric individuals with ALL and its susceptibility to nystatin and amphotericin B |
| title_fullStr | Candida on oral cavity of pediatric individuals with ALL and its susceptibility to nystatin and amphotericin B |
| title_full_unstemmed | Candida on oral cavity of pediatric individuals with ALL and its susceptibility to nystatin and amphotericin B |
| title_short | Candida on oral cavity of pediatric individuals with ALL and its susceptibility to nystatin and amphotericin B |
| title_sort | candida on oral cavity of pediatric individuals with all and its susceptibility to nystatin and amphotericin b |
| topic | candida precursor cell lymphoblastic leukemia-lymphoma prevalence |
| url | https://www.scielo.br/scielo.php?script=sci_arttext&pid=S1981-86372019000100309&lng=en&nrm=iso&tlng=en |
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