Endothelial Progenitors Exist within the Kidney and Lung Mesenchyme.
The renal endothelium has been debated as arising from resident hemangioblast precursors that transdifferentiate from the nephrogenic mesenchyme (vasculogenesis) and/or from invading vessels (angiogenesis). While the Foxd1-positive renal cortical stroma has been shown to differentiate into cells tha...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3688860?pdf=render |
| _version_ | 1819128088301142016 |
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| author | Sunder Sims-Lucas Caitlin Schaefer Daniel Bushnell Jacqueline Ho Alison Logar Edward Prochownik George Gittes Carlton M Bates |
| author_facet | Sunder Sims-Lucas Caitlin Schaefer Daniel Bushnell Jacqueline Ho Alison Logar Edward Prochownik George Gittes Carlton M Bates |
| author_sort | Sunder Sims-Lucas |
| collection | DOAJ |
| description | The renal endothelium has been debated as arising from resident hemangioblast precursors that transdifferentiate from the nephrogenic mesenchyme (vasculogenesis) and/or from invading vessels (angiogenesis). While the Foxd1-positive renal cortical stroma has been shown to differentiate into cells that support the vasculature in the kidney (including vascular smooth muscle and pericytes) it has not been considered as a source of endothelial cell progenitors. In addition, it is unclear if Foxd1-positive mesenchymal cells in other organs such as the lung have the potential to form endothelium. This study examines the potential for Foxd1-positive cells of the kidney and lung to give rise to endothelial progenitors. We utilized immunofluorescence (IF) and fluorescence-activated cell sorting (FACS) to co-label Foxd1-expressing cells (including permanently lineage-tagged cells) with endothelial markers in embryonic and postnatal mice. We also cultured FACsorted Foxd1-positive cells, performed in vitro endothelial cell tubulogenesis assays and examined for endocytosis of acetylated low-density lipoprotein (Ac-LDL), a functional assay for endothelial cells. Immunofluorescence and FACS revealed that a subset of Foxd1-positive cells from kidney and lung co-expressed endothelial cell markers throughout embryogenesis. In vitro, cultured embryonic Foxd1-positive cells were able to differentiate into tubular networks that expressed endothelial cell markers and were able to endocytose Ac-LDL. IF and FACS in both the kidney and lung revealed that lineage-tagged Foxd1-positive cells gave rise to a significant portion of the endothelium in postnatal mice. In the kidney, the stromal-derived cells gave rise to a portion of the peritubular capillary endothelium, but not of the glomerular or large vessel endothelium. These findings reveal the heterogeneity of endothelial cell lineages; moreover, Foxd1-positive mesenchymal cells of the developing kidney and lung are a source of endothelial progenitors that are likely critical to patterning the vasculature. |
| first_indexed | 2024-12-22T08:22:16Z |
| format | Article |
| id | doaj.art-af53d293bc5d4def9c407868aea1ca7e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T08:22:16Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-af53d293bc5d4def9c407868aea1ca7e2022-12-21T18:32:43ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0186e6599310.1371/journal.pone.0065993Endothelial Progenitors Exist within the Kidney and Lung Mesenchyme.Sunder Sims-LucasCaitlin SchaeferDaniel BushnellJacqueline HoAlison LogarEdward ProchownikGeorge GittesCarlton M BatesThe renal endothelium has been debated as arising from resident hemangioblast precursors that transdifferentiate from the nephrogenic mesenchyme (vasculogenesis) and/or from invading vessels (angiogenesis). While the Foxd1-positive renal cortical stroma has been shown to differentiate into cells that support the vasculature in the kidney (including vascular smooth muscle and pericytes) it has not been considered as a source of endothelial cell progenitors. In addition, it is unclear if Foxd1-positive mesenchymal cells in other organs such as the lung have the potential to form endothelium. This study examines the potential for Foxd1-positive cells of the kidney and lung to give rise to endothelial progenitors. We utilized immunofluorescence (IF) and fluorescence-activated cell sorting (FACS) to co-label Foxd1-expressing cells (including permanently lineage-tagged cells) with endothelial markers in embryonic and postnatal mice. We also cultured FACsorted Foxd1-positive cells, performed in vitro endothelial cell tubulogenesis assays and examined for endocytosis of acetylated low-density lipoprotein (Ac-LDL), a functional assay for endothelial cells. Immunofluorescence and FACS revealed that a subset of Foxd1-positive cells from kidney and lung co-expressed endothelial cell markers throughout embryogenesis. In vitro, cultured embryonic Foxd1-positive cells were able to differentiate into tubular networks that expressed endothelial cell markers and were able to endocytose Ac-LDL. IF and FACS in both the kidney and lung revealed that lineage-tagged Foxd1-positive cells gave rise to a significant portion of the endothelium in postnatal mice. In the kidney, the stromal-derived cells gave rise to a portion of the peritubular capillary endothelium, but not of the glomerular or large vessel endothelium. These findings reveal the heterogeneity of endothelial cell lineages; moreover, Foxd1-positive mesenchymal cells of the developing kidney and lung are a source of endothelial progenitors that are likely critical to patterning the vasculature.http://europepmc.org/articles/PMC3688860?pdf=render |
| spellingShingle | Sunder Sims-Lucas Caitlin Schaefer Daniel Bushnell Jacqueline Ho Alison Logar Edward Prochownik George Gittes Carlton M Bates Endothelial Progenitors Exist within the Kidney and Lung Mesenchyme. PLoS ONE |
| title | Endothelial Progenitors Exist within the Kidney and Lung Mesenchyme. |
| title_full | Endothelial Progenitors Exist within the Kidney and Lung Mesenchyme. |
| title_fullStr | Endothelial Progenitors Exist within the Kidney and Lung Mesenchyme. |
| title_full_unstemmed | Endothelial Progenitors Exist within the Kidney and Lung Mesenchyme. |
| title_short | Endothelial Progenitors Exist within the Kidney and Lung Mesenchyme. |
| title_sort | endothelial progenitors exist within the kidney and lung mesenchyme |
| url | http://europepmc.org/articles/PMC3688860?pdf=render |
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